Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Nguyen, D.T.N.; Lee, O.K.; Lim, C.; Lee, J.; Na, J.G.; Lee, E.Y.
    Metabolic engineering of type II methanotroph, Methylosinus trichosporium OB3b, for production of 3-hydroxypropionic acid from methane via a malonyl-CoA reductase-dependent pathway (2020), Metab. Eng., 59, 142-150 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.298 gene mcr, recombinant expression of the Chloroflexus aurantiacus enzyme in Methylosinus trichosporium strain OB3b, subcloning in Escherichia coli strain DH5alpha Chloroflexus aurantiacus
1.2.1.75 expressed in Methylosinus trichosporium strain OB3b Chloroflexus aurantiacus
1.2.1.75 gene mcr, recombinant expression of the Chloroflexus aurantiacus enzyme in Methylosinus trichosporium strain OB3b, subcloning in Escherichia coli strain DH5alpha Chloroflexus aurantiacus

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.298 additional information engineering of type II methanotroph Methylosinus trichosporium strain OB3b for 3-hydroxypropionic acid (3HP) production by reconstructing malonyl-CoA pathway through heterologous expression of Chloroflexus aurantiacus malonyl-CoA reductase (MCR), a bifunctional enzyme. Engineering of the supply of malonyl-CoA precursors by overexpressing endogenous acetyl-CoA carboxylase (ACC), substantially enhancing the production of 3HP. Overexpression of biotin protein ligase (BPL) and malic enzyme (NADP+-ME) leads to 22.7% and 34.5% increase, respectively, in 3HP titer in ACC-overexpressing cells. Also, the acetyl-CoA carboxylation bypass route is reconstructed to improve 3HP productivity. Coexpression of methylmalonyl-CoA carboxyltransferase (MMC) of Propionibacterium freudenreichii and phosphoenolpyruvate carboxylase (PEPC), which provides the MMC precursor, further improves the 3HP titer. The highest 3HP production of 49 mg/l in the OB3b-MCRMP strain overexpressing MCR, MMC and PEPC results in a 2.4fold improvement of titer compared with that in the only MCR-overexpressing strain. 60.59 mg/l of 3HP are obatined in 42 h using the OB3b-MCRMP strain through bioreactor operation, with a 6.36fold increase of volumetric productivity compared than that in the flask cultures Chloroflexus aurantiacus
1.2.1.75 additional information engineering of type II methanotroph Methylosinus trichosporium strain OB3b for 3-hydroxypropionic acid (3HP) production by reconstructing malonyl-CoA pathway through heterologous expression of Chloroflexus aurantiacus malonyl-CoA reductase (MCR), a bifunctional enzyme. Engineering of the supply of malonyl-CoA precursors by overexpressing endogenous acetyl-CoA carboxylase (ACC), substantially enhancing the production of 3HP. Overexpression of biotin protein ligase (BPL) and malic enzyme (NADP+-ME) leads to 22.7% and 34.5% increase, respectively, in 3HP titer in ACC-overexpressing cells. Also, the acetyl-CoA carboxylation bypass route is reconstructed to improve 3HP productivity. Coexpression of methylmalonyl-CoA carboxyltransferase (MMC) of Propionibacterium freudenreichii and phosphoenolpyruvate carboxylase (PEPC), which provides the MMC precursor, further improves the 3HP titer. The highest 3HP production of 49 mg/l in the OB3b-MCRMP strain overexpressing MCR, MMC and PEPC results in a 2.4fold improvement of titer compared with that in the only MCR-overexpressing strain. 60.59 mg/l of 3HP are obatined in 42 h using the OB3b-MCRMP strain through bioreactor operation, with a 6.36fold increase of volumetric productivity compared than that in the flask cultures Chloroflexus aurantiacus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.298 malonate semialdehyde + NADPH + H+ Chloroflexus aurantiacus
-
 3-hydroxypropanoate + NADP+
-
 ?
1.2.1.75 malonate semialdehyde + NADPH + H+ Chloroflexus aurantiacus
-
 3-hydroxypropionic acid + NADP+
-
 ?
1.2.1.75 malonyl-CoA + NADPH + H+ Chloroflexus aurantiacus
-
 malonate semialdehyde + CoA + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.298 Chloroflexus aurantiacus Q6QQP7 bifunctional enzyme
-
1.2.1.75 Chloroflexus aurantiacus Q6QQP7
-
-
1.2.1.75 Chloroflexus aurantiacus Q6QQP7 bifunctional enzyme
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.298 malonate semialdehyde + NADPH + H+
-
 Chloroflexus aurantiacus 3-hydroxypropanoate + NADP+
-
 ?
1.2.1.75 malonate semialdehyde + NADPH + H+
-
 Chloroflexus aurantiacus 3-hydroxypropionic acid + NADP+
-
 ?
1.2.1.75 malonyl-CoA + NADPH + H+
-
 Chloroflexus aurantiacus malonate semialdehyde + CoA + NADP+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.298 MCR
-
 Chloroflexus aurantiacus
1.2.1.75 malonyl-CoA reductase
-
 Chloroflexus aurantiacus
1.2.1.75 MCR
-
 Chloroflexus aurantiacus
1.2.1.75 More see also EC 1.1.1.298 Chloroflexus aurantiacus

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.75 NADPH
-
 Chloroflexus aurantiacus

General Information

EC Number General Information Comment Organism
1.1.1.298 metabolism 3-hydroxypropionic acid (3HP) production via MCR dependent pathway, overview. The bifunctional enzyme shows malonate semialdehyde reduction activity and also malonyl-CoA reduction activity, EC 1.2.1.75 Chloroflexus aurantiacus
1.2.1.75 metabolism 3-hydroxypropionic acid (3HP) production via MCR dependent pathway, overview. The bifunctional enzyme shows malonate semialdehyde reduction activity, EC 1.1.1.298, and also malonyl-CoA reduction activity Chloroflexus aurantiacus