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Literature summary extracted from

  • Wang, Y.; Hou, Y.; Wang, Y.; Zheng, L.; Xu, X.; Pan, K.; Li, R.; Wang, Q.
    A novel cold-adapted leucine dehydrogenase from antarctic sea-ice bacterium Pseudoalteromonas sp. ANT178 (2018), Mar. Drugs, 16, 359 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.1.9 expression in Escherichia coli Pseudoalteromonas sp. ANT 178

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.4.1.9 homology modeling of structure. Enzyme exhibits several cold-adapted features Pseudoalteromonas sp. ANT 178

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.1.9 Cd2+ 1 mM, 8% residual activity Pseudoalteromonas sp. ANT 178
1.4.1.9 Cr2+ 1 mM, 13% residual activity Pseudoalteromonas sp. ANT 178
1.4.1.9 Cu2+ 1 mM, 61% residual activity Pseudoalteromonas sp. ANT 178
1.4.1.9 EDTA 1 mM, 92% residual activity Pseudoalteromonas sp. ANT 178
1.4.1.9 Fe2+ 1 mM, 75% residual activity Pseudoalteromonas sp. ANT 178
1.4.1.9 Hg2+ 1 mM, 29% residual activity Pseudoalteromonas sp. ANT 178
1.4.1.9 Pb2+ 1 mM, no residual activity Pseudoalteromonas sp. ANT 178
1.4.1.9 Thiourea 1 mM, 52% residual activity Pseudoalteromonas sp. ANT 178
1.4.1.9 Zn2+ 1 mM, 80% residual activity Pseudoalteromonas sp. ANT 178

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.1.9 0.33
-
 L-leucine pH 9, 30°C Pseudoalteromonas sp. ANT 178
1.4.1.9 0.48
-
 L-tyrosine pH 9, 30°C Pseudoalteromonas sp. ANT 178
1.4.1.9 0.64
-
 L-proline pH 9, 30°C Pseudoalteromonas sp. ANT 178
1.4.1.9 0.75
-
 DL-methionine pH 9, 30°C Pseudoalteromonas sp. ANT 178
1.4.1.9 0.84
-
 L-arginine pH 9, 30°C Pseudoalteromonas sp. ANT 178

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.4.1.9 NaCl 2 M NaCl, 140% of initial activity Pseudoalteromonas sp. ANT 178

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.9 Pseudoalteromonas sp. ANT 178 A0A385GJJ6
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.1.9 275
-
 pH 9, 30°C, substrate L-leucine Pseudoalteromonas sp. ANT 178

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.9 DL-methionine + H2O + NAD+
-
 Pseudoalteromonas sp. ANT 178 3-methylthio-2-oxobutanoate + NH3 + NADH + H+
-
 ?
1.4.1.9 L-arginine + H2O + NAD+
-
 Pseudoalteromonas sp. ANT 178 5-[(diaminomethyl)amino]-2-oxopentanoic acid + NH3 + NADH + H+
-
 ?
1.4.1.9 L-leucine + H2O + NAD+
-
 Pseudoalteromonas sp. ANT 178 4-methyl-2-oxopentanoate + NH3 + NADH + H+
-
 ?
1.4.1.9 L-proline + H2O + NAD+
-
 Pseudoalteromonas sp. ANT 178 ? + NH3 + NADH + H+
-
 ?
1.4.1.9 L-tyrosine + H2O + NAD+
-
 Pseudoalteromonas sp. ANT 178 ? + NH3 + NADH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.4.1.9 ? x * 42600, calculated from sequence, x * 44000, SDS-PAGE, recombinant protein Pseudoalteromonas sp. ANT 178

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.4.1.9 30
-
-
 Pseudoalteromonas sp. ANT 178

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.1.9 30
-
 120 min, 85% residual activity Pseudoalteromonas sp. ANT 178

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.4.1.9 14.1
-
 L-arginine pH 9, 30°C Pseudoalteromonas sp. ANT 178
1.4.1.9 16.6
-
 DL-methionine pH 9, 30°C Pseudoalteromonas sp. ANT 178
1.4.1.9 20.8
-
 L-proline pH 9, 30°C Pseudoalteromonas sp. ANT 178
1.4.1.9 26.4
-
 L-tyrosine pH 9, 30°C Pseudoalteromonas sp. ANT 178
1.4.1.9 30.13
-
 L-leucine pH 9, 30°C Pseudoalteromonas sp. ANT 178

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.1.9 9
-
-
 Pseudoalteromonas sp. ANT 178

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.4.1.9 16.8
-
 L-arginine pH 9, 30°C Pseudoalteromonas sp. ANT 178
1.4.1.9 22.1
-
 DL-methionine pH 9, 30°C Pseudoalteromonas sp. ANT 178
1.4.1.9 32.5
-
 L-proline pH 9, 30°C Pseudoalteromonas sp. ANT 178
1.4.1.9 55
-
 L-tyrosine pH 9, 30°C Pseudoalteromonas sp. ANT 178
1.4.1.9 91.3
-
 L-leucine pH 9, 30°C Pseudoalteromonas sp. ANT 178