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Literature summary extracted from

  • Zhang, S.; Wu, S.; Niu, C.; Liu, D.; Yan, T.; Tian, Y.; Liu, S.; Xie, K.; Li, Z.; Wang, Y.; Zhao, W.; Dong, Z.; Zhu, T.; Hou, Q.; Ma, B.; An, X.; Li, J.; Wan, X.
    ZmMs25 encoding a plastid-localized fatty acyl reductase is critical for anther and pollen development in maize (2021), J. Exp. Bot., 72, 4298-4318 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.84 expressed in Escherichia coli BL21 cells Zea mays

Protein Variants

EC Number Protein Variants Comment Organism
1.2.1.84 G101A the activity of the variant is decreased by 62.2% after incubation with short-chain lauroyl-CoA at pH 8.0 and 30°C for 180 min, but 100% activity is lost when using long-chain palmitoyl- and stearoyl-CoAs as the substrate Zea mays
1.2.1.84 G104A the activities of the variant are decreased by 56.1%, 65.9%, and 68.3% toward lauroyl-, palmitoyl-, and stearoyl-CoAs, respectively after 180 min incubation, compared with the wild type enzyme Zea mays
1.2.1.84 Y327F the activities of the variant drop to 0-6.6% toward lauroyl-, palmitoyl-, and stearoyl-CoAs after 180 min incubation, compared with the wild type enzyme Zea mays
1.2.1.84 Y331I the activities of the variant are decreased by 86.2%, 88.3%, and 93.2% toward lauroyl-, palmitoyl-, and stearoyl-CoAs, respectively, compared with the wild type enzyme Zea mays

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.2.1.84 plastid
-
 Zea mays 9536
-

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.84 Zea mays
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.84 Ni affinity column chromatography Zea mays

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.1.84 anther
-
 Zea mays
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.84 lauroyl-CoA + 2 NADPH + 2 H+
-
 Zea mays dodecanal + CoA + 2 NADP+
-
 ?
1.2.1.84 palmitoyl-CoA + 2 NADPH + 2 H+
-
 Zea mays hexadecanal + CoA + 2 NADP+
-
 ?
1.2.1.84 stearoyl-CoA + 2 NADPH + 2 H+
-
 Zea mays octadecanal + CoA + 2 NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.1.84 ? x * 106000, maltose-binding protein-tagged enzyme, SDS-PAGE Zea mays

Synonyms

EC Number Synonyms Comment Organism
1.2.1.84 Ms25
-
 Zea mays

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.84 NADPH
-
 Zea mays

Expression

EC Number Organism Comment Expression
1.2.1.84 Zea mays the enzyme gene is directly activated and regulated by MYB84 up

General Information

EC Number General Information Comment Organism
1.2.1.84 malfunction enzyme mutation has significant effects on reducing cutin monomers and internal lipids, and altering the composition of cuticular wax in anthers. Moreover, loss of function of the enzyme significantly affects the expression of its four paralogous genes and five cloned lipid metabolic male-sterility genes in maize Zea mays
1.2.1.84 physiological function the enzyme is critical for anther and pollen development in maize Zea mays