EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.2.7.6 | NaCl | enzyme shows partial substrate inhibition, inhibition is alleviated completely by a 1M NaCl resulting in increased enzyme activity at high substrate concentrations | Pyrococcus furiosus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.2.7.6 | D-glyceraldehyde 3-phosphate | partial substrate inhibition, inhibition is alleviated completely by a 1M NaCl resulting in increased enzyme activity at high substrate concentrations | Pyrococcus furiosus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.2.7.B2 | 0.22 | - |
formaldehyde | pH 8.4, 25°C | Pyrococcus furiosus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.2.7.B2 | Pyrococcus furiosus | Q8U1K3 | - |
- |
1.2.7.6 | Pyrococcus furiosus | Q8U3K2 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.7.B2 | formaldehyde + H2O + 2 oxidized methyl viologen | - |
Pyrococcus furiosus | formate + 2 reduced methyl viologen + 2 H+ | - |
? | |
1.2.7.6 | D-glyceraldehyde 3-phosphate + H2O + 2 oxidized benzyl viologen | D-glyceraldehyde 3-phosphate is the only substrate oxidized by GAPOR, and the kinetics of the enzyme are unaffected by the presence of L-glyceraldehyde 3-phosphate | Pyrococcus furiosus | 3-phospho-D-glycerate + 2 H+ + 2 reduced benzyl viologen | - |
? | |
1.2.7.6 | additional information | substrate glyceraldehyde-3-phosphate degrades at 60°C by non-enzymatic elimination of the phosphate group to methylglyoxal with a half-life of 6.5 min. Methylglyoxal is not a substrate or inhibitor of GAPOR | Pyrococcus furiosus | ? | - |
- |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.2.7.B2 | FOR | - |
Pyrococcus furiosus |
1.2.7.6 | Gor | - |
Pyrococcus furiosus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.2.7.6 | 9 | - |
at pH 9, the substrate is a divalent anion | Pyrococcus furiosus |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.2.7.B2 | physiological function | enzyme exhibits a significant primary kinetic isotope effect of the apparent Vmax for the deuterated substrate, formaldehyde-d2, which shows that the rate-determining step involves a C-H bond break from the aldehyde | Pyrococcus furiosus |