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Literature summary extracted from

  • Kato, Y.; Yoshida, S.; Xie, S.X.; Asano, Y.
    Aldoxime dehydratase co-existing with nitrile hydratase and amidase in the iron-type nitrile hydratas(E)-producer Rhodococcus sp. N-771 (2004), J. Biosci. Bioeng., 97, 250-259 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.8.1.2 2-mercaptoethanol the enzyme activity is enhanced 1.8fold by 1 mM Rhodococcus sp. N-771
4.8.1.2 cysteamine the enzyme activity is enhanced 2.3fold by 1 mM Rhodococcus sp. N-771
4.8.1.2 duroquinone the enzyme activity is increased 6.7fold in the absence of Na2S Rhodococcus sp. N-771
4.8.1.2 FAD the enzyme activity is enhanced 3fold by 1 mM Rhodococcus sp. N-771
4.8.1.2 FMN the enzyme activity is enhanced 7.2fold by 1 mM Rhodococcus sp. N-771
4.8.1.2 L-cysteine the enzyme activity is enhanced 2.4fold by 1 mM Rhodococcus sp. N-771
4.8.1.2 additional information the activity is not increased by the additions of NaHSO3, Na2SO4, NaHSO4or Na2S2O7. The following coenzymes do not affect the reaction: pantothenate, phosphoenolpyruvate, NaF, pyridoxal, dehydroascorbate, glutathione, glutathione disulfide, NAD(P)(H), D-glucose 6-phosphate, glutathione disulfide, dehydroascorbic acid, CoA-SH, AXP, GXP, and IXP Rhodococcus sp. N-771
4.8.1.2 Na2S the enzyme activity is enhanced 4.5fold by 1 mM Rhodococcus sp. N-771
4.8.1.2 Na2S2O4 the enzyme activity is enhanced 2.3fold by 1 mM Rhodococcus sp. N-771
4.8.1.2 Na2S2O5 the enzyme activity is enhanced 13.8fold by 1 mM Rhodococcus sp. N-771
4.8.1.2 Na2SO3 the enzyme activity is enhanced 5.4fold by 1 mM Rhodococcus sp. N-771
4.8.1.2 riboflavin the enzyme activity is enhanced 3.4fold by 1 mM Rhodococcus sp. N-771
4.8.1.2 thioglycerol the enzyme activity is enhanced 3.5fold by 1 mM Rhodococcus sp. N-771
4.8.1.2 vitamin K3 the enzyme activity is increased 23fold in the presence of Na2S Rhodococcus sp. N-771

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.8.1.2 expressed in Escherichia coli BL21 Star (DE3) cells Rhodococcus sp. N-771

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.8.1.2 (E)-benzaldoxime
-
 Rhodococcus sp. N-771
4.8.1.2 2-phenylenediamine slight inhibition in the absence of Na2S Rhodococcus sp. N-771
4.8.1.2 acetophenone
-
 Rhodococcus sp. N-771
4.8.1.2 As3+
-
 Rhodococcus sp. N-771
4.8.1.2 Cd2+
-
 Rhodococcus sp. N-771
4.8.1.2 Cu+
-
 Rhodococcus sp. N-771
4.8.1.2 Cu2+
-
 Rhodococcus sp. N-771
4.8.1.2 D-cycloserine 35% inhibition at 0.1 mM in the absence of Na2S Rhodococcus sp. N-771
4.8.1.2 digallol slight inhibition in the absence of Na2S Rhodococcus sp. N-771
4.8.1.2 EDTA 45% inhibition at 0.1 mM in the absence of Na2S Rhodococcus sp. N-771
4.8.1.2 guaiacol slight inhibition in the absence of Na2S Rhodococcus sp. N-771
4.8.1.2 Hg2+
-
 Rhodococcus sp. N-771
4.8.1.2 additional information not inhibited by hydrazine Rhodococcus sp. N-771
4.8.1.2 phenylacetone
-
 Rhodococcus sp. N-771
4.8.1.2 phenylhydrazine
-
 Rhodococcus sp. N-771
4.8.1.2 Tiron 55% inhibition at 0.1 mM in the absence of Na2S Rhodococcus sp. N-771

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.8.1.2 Co2+ the enzyme activity is increased 14fold by 1 mM Co2+ Rhodococcus sp. N-771
4.8.1.2 D-cycloserine the enzyme activity is increased 1.6fold at 0.1 mM D-cycloserine in the presence of Na2S Rhodococcus sp. N-771
4.8.1.2 EDTA the enzyme activity is increased 2.1fold at 0.1 mM EDTA in the presence of Na2S Rhodococcus sp. N-771
4.8.1.2 Fe2+ the enzyme activity is increased 23.5fold by 1 mM Fe2+ Rhodococcus sp. N-771
4.8.1.2 Fe3+ the enzyme activity is increased 24.3fold by 1 mM Fe3+ Rhodococcus sp. N-771
4.8.1.2 additional information the following metal ions do not inhibit the enzyme activity under any reaction conditions: Li+, B4+, Na+, Mg2+, K+, Ca2+, Mn2+, Ti2+, Cr3+, Rb+, Mo6+, Ag+, Cs+, Ba2+ and Pb2+ Rhodococcus sp. N-771
4.8.1.2 Sn2+ the enzyme activity is increased 3.6fold by 1 mM Sn2+ Rhodococcus sp. N-771
4.8.1.2 Tiron the enzyme activity is increased 7.2fold at 0.1 mM Tiron in the presence of Na2S Rhodococcus sp. N-771
4.8.1.2 Zn2+ the enzyme activity is increased 2.5fold by 1 mM Zn2+ Rhodococcus sp. N-771

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.8.1.2 80000
-
 gel filtration Rhodococcus sp. N-771

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.8.1.2 an aliphatic aldoxime Rhodococcus sp. N-771 the enzyme preferentially acts on aliphatic-type substrates an aliphatic nitrile + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.8.1.2 Rhodococcus sp. N-771
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.8.1.2 ammonium sulfate precipitation, DEAE column chromatography, butyl-Toyopearl column chromatography and Superdex 200 gel filtration Rhodococcus sp. N-771

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.8.1.2 3.92
-
 after 7.1fold purification, at pH 8.0 and 30°C Rhodococcus sp. N-771

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.8.1.2 (E)-4-chlorobenzaldoxime 0.331% activity in the presence of Na2S compared to (Z)-phenylacetaldehyde oxime Rhodococcus sp. N-771 ?
-
 ?
4.8.1.2 (E)-4-tolualdoxime 0.488% activity in the presence of Na2S compared to (Z)-phenylacetaldehyde oxime Rhodococcus sp. N-771 ?
-
 ?
4.8.1.2 (E)-benzaldoxime 1.92% activity in the presence of Na2S compared to (Z)-phenylacetaldehyde oxime Rhodococcus sp. N-771 ?
-
 ?
4.8.1.2 (E)-furfurylaldoxime 0.504% activity in the presence of Na2S compared to (Z)-phenylacetaldehyde oxime Rhodococcus sp. N-771 ?
-
 ?
4.8.1.2 (E)-pyridine-3-aldoxime 0.535% activity in the presence of Na2S compared to (Z)-phenylacetaldehyde oxime Rhodococcus sp. N-771 ?
-
 ?
4.8.1.2 (E)-thiophene-2-acetaldoxime 26.3% activity in the presence of Na2S compared to (Z)-phenylacetaldehyde oxime Rhodococcus sp. N-771 ?
-
 ?
4.8.1.2 (E)-thiophene-2-carboxaldoxime 3.37% activity in the presence of Na2S compared to (Z)-phenylacetaldehyde oxime Rhodococcus sp. N-771 ?
-
 ?
4.8.1.2 (E/Z)-2-phenylpropionaldoxime 49.8% activity in the presence of Na2S compared to (Z)-phenylacetaldehyde oxime Rhodococcus sp. N-771 (E/Z)-2-phenylpropiononitrile + H2O
-
 ?
4.8.1.2 (E/Z)-4-phenylbutyraldoxime 29.1% activity in the presence of Na2S compared to (Z)-phenylacetaldehyde oxime Rhodococcus sp. N-771 phenylbutyronitrile + H2O
-
 ?
4.8.1.2 (E/Z)-cinnamaldehyde oxime 4.28% activity in the presence of Na2S compared to (Z)-phenylacetaldehyde oxime Rhodococcus sp. N-771 ?
-
 ?
4.8.1.2 (E/Z)-cyclohexanecarboxaldehyde oxime 157% activity in the presence of Na2S compared to (Z)-phenylacetaldehyde oxime Rhodococcus sp. N-771 ?
-
 ?
4.8.1.2 (E/Z)-indoleacetaldoxime 41.7% activity in the presence of Na2S compared to (Z)-phenylacetaldehyde oxime Rhodococcus sp. N-771 indoleacetonitrile + H2O
-
 ?
4.8.1.2 (E/Z)-isobutyraldoxime 89.6% activity in the presence of Na2S compared to (Z)-phenylacetaldehyde oxime Rhodococcus sp. N-771 ?
-
 ?
4.8.1.2 (E/Z)-isocapronaldoxime 48.7% activity in the presence of Na2S compared to (Z)-phenylacetaldehyde oxime Rhodococcus sp. N-771 isocapronitrile + H2O
-
 ?
4.8.1.2 (E/Z)-isovaleraldoxime 120% activity in the presence of Na2S compared to (Z)-phenylacetaldehyde oxime Rhodococcus sp. N-771 isovaleronitrile + H2O
-
 ?
4.8.1.2 (E/Z)-mandelaldoxime 11.7% activity in the presence of Na2S compared to (Z)-phenylacetaldehyde oxime Rhodococcus sp. N-771 mandeloacetonitrile + H2O
-
 ?
4.8.1.2 (E/Z)-n-butyraldoxime 100% activity in the presence of Na2S compared to (Z)-phenylacetaldehyde oxime Rhodococcus sp. N-771 n-butyronitrile + H2O
-
 ?
4.8.1.2 (E/Z)-n-capronaldoxime 85.5% activity in the presence of Na2S compared to (Z)-phenylacetaldehyde oxime Rhodococcus sp. N-771 n-caprononitrile + H2O
-
 ?
4.8.1.2 (E/Z)-n-valeraldoxime 68.3% activity in the presence of Na2S compared to (Z)-phenylacetaldehyde oxime Rhodococcus sp. N-771 n-valeronitrile + H2O
-
 ?
4.8.1.2 (E/Z)-propionaldoxime 87.7% activity in the presence of Na2S compared to (Z)-phenylacetaldehyde oxime Rhodococcus sp. N-771 propionitrile + H2O
-
 ?
4.8.1.2 (Z)-3-phenylpropionaldoxime 69.6% activity in the presence of Na2S compared to (Z)-phenylacetaldehyde oxime Rhodococcus sp. N-771 3-phenylpropiononitrile
-
 ?
4.8.1.2 (Z)-4-chlorophenylacetaldoxime 3.39% activity in the presence of Na2S compared to (Z)-phenylacetaldehyde oxime Rhodococcus sp. N-771 4-chlorophenylacetonitrile + H2O
-
 ?
4.8.1.2 (Z)-naphthoacetaldoxime 2.71% activity in the presence of Na2S compared to (Z)-phenylacetaldehyde oxime Rhodococcus sp. N-771 naphthoacetonitrile + H2O
-
 ?
4.8.1.2 (Z)-phenylacetaldehyde oxime 100% activity in the presence of Na2S Rhodococcus sp. N-771 phenylacetonitrile + H2O
-
 ?
4.8.1.2 an aliphatic aldoxime the enzyme preferentially acts on aliphatic-type substrates Rhodococcus sp. N-771 an aliphatic nitrile + H2O
-
 ?
4.8.1.2 additional information the following compounds are inert as substrates: ((E/Z))-diphenylacetaldoxime, ((E/Z))-4-hydroxyphenylacetaldoxime, (E)-1-naphthoaldoxime, (E)-anisaldoxime, (E)-quinolin(E)-2-carboxaldehyde oxime, (E)-terephthalaldehyde oxime, (E)-isophthalaldehyde oxime, (E)-pyrazinecarboxaldoxime, (E)-indol(E)-3-carboxaldehyde oxime, (Z)-crotonaldoxime, ((E/Z))-methacrylaldoxime, ((E/Z))-O-benzylphenylacetaldehyde oxime, (E)-phenylacetaldehyde oxime hydrazone, ((E/Z))-O-acetyl-phenylacetaldehyde oxime, ((E/Z))-phenylacetone oxime and ((E/Z))-acetophenone oxime Rhodococcus sp. N-771 ?
-
-

Subunits

EC Number Subunits Comment Organism
4.8.1.2 homodimer 2 * 39868, calculated from amino acid sequence Rhodococcus sp. N-771

Synonyms

EC Number Synonyms Comment Organism
4.8.1.2 OxdRE
-
 Rhodococcus sp. N-771

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.8.1.2 30
-
-
 Rhodococcus sp. N-771

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.8.1.2 8
-
-
 Rhodococcus sp. N-771

Cofactor

EC Number Cofactor Comment Organism Structure
4.8.1.2 heme b
-
 Rhodococcus sp. N-771