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Literature summary extracted from

  • Ellenbogen, J.; Jiang, R.; Kountz, D.; Zhang, L.; Krzycki, J.
    The MttB superfamily member MtyB from the human gut symbiont Eubacterium limosum is a cobalamin-dependent gamma-butyrobetaine methyltransferase (2021), J. Biol. Chem., 297, 101327 .
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.B141 3.5
-
 gamma-butyrobetaine pH 7.2, 37°C Eubacterium limosum
2.1.1.B141 4
-
 L-carnitine pH 7.2, 37°C Eubacterium limosum

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.B141 Eubacterium limosum A0A317RJG9
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.B141 choline + [corrinoid-binding protein Co(I)-MtqC]
-
 Eubacterium limosum [methyl-corrinoid-protein Co(I)-MtqC] + 2-(dimethylamino)ethan-1-ol
-
 ?
2.1.1.B141 gamma-butyrobetaine + [corrinoid-binding protein Co(I)-MtqC]
-
 Eubacterium limosum [methyl-corrinoid-protein Co(I)-MtqC] + 4-(dimethylamino)butanoate
-
 ?
2.1.1.B141 glycine betaine + [corrinoid-binding protein Co(I)-MtqC]
-
 Eubacterium limosum [methyl-corrinoid-protein Co(I)-MtqC] + N-dimethylglycine
-
 ?
2.1.1.B141 L-carnitine + [corrinoid-binding protein Co(I)-MtqC]
-
 Eubacterium limosum [methyl-corrinoid-protein Co(I)-MtqC] + L-norcarnitine
-
 ?
2.1.1.B141 additional information demethylation of other methylated amines occurs at much lower rates than observed with gamma-butyrobetaine or L-carnitine as a substrate Eubacterium limosum ?
-
-
2.1.1.B141 proline betaine + [corrinoid-binding protein Co(I)-MtqC]
-
 Eubacterium limosum [methyl-corrinoid-protein Co(I)-MtqC] + N-methylproline
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.B141 C7955_113110
-
 Eubacterium limosum
2.1.1.B141 MtyB
-
 Eubacterium limosum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.1.B141 3
-
 gamma-butyrobetaine pH 7.2, 37°C Eubacterium limosum
2.1.1.B141 6.8
-
 L-carnitine pH 7.2, 37°C Eubacterium limosum

Expression

EC Number Organism Comment Expression
2.1.1.B141 Eubacterium limosum MtyB constitutes 0.9 mol% from gamma-butyrobetaine-grown cells but is not detectable in the proteome of cells grown on lactate additional information

General Information

EC Number General Information Comment Organism
2.1.1.B141 physiological function proteins MtyB, MtqC (a corrinoid-binding protein), and MtqA (a corrinoid:tetrahydrofolate methyltransferase) are significantly elevated in gamma-butyrobetaine-grown cells. Together, they act as a gamma-butyrobetaine:tetrahydrofolate methyltransferase system, forming a key intermediate of acetogenesis. Recombinant MtyB acts as a gamma-butyrobetaine:MtqC methyltransferase but cannot methylate free cobalamin cofactor Eubacterium limosum