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Literature summary extracted from

  • Radka, C.D.; Frank, M.W.; Yao, J.; Seetharaman, J.; Miller, D.J.; Rock, C.O.
    The genome of a Bacteroidetes inhabitant of the human gut encodes a structurally distinct enoyl-acyl carrier protein reductase (FabI) (2020), J. Biol. Chem., 295, 7635-7652 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.9 expression in Escherichia coli Alistipes finegoldii

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.3.1.9 crystal structure at 1.72 A resolution, protein adopts a Rossmann fold. FabI/NADH crystal structure at 1.86 A resolution, the protein undergoes a large conformational change to participate in covering the NADH-binding pocket Alistipes finegoldii

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.1.9 triclosan
-
 Alistipes finegoldii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.1.9 0.022
-
 NADH K0.5 value, Hill coefficient 2.4, pH 8, 30°C Alistipes finegoldii
1.3.1.9 0.457
-
 crotonoyl-CoA K0.5 value, Hill coefficient 1.5, pH 8, 30°C Alistipes finegoldii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.1.9 141000
-
 sedimentation velocity analysis Alistipes finegoldii
1.3.1.9 145000
-
 gel filtration Alistipes finegoldii

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.9 Alistipes finegoldii A0A174E195
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.9 crotonoyl-CoA + NADH + H+
-
 Alistipes finegoldii butanoyl-CoA + NAD+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.1.9 tetramer 4 * 34000, SDS-PAGE Alistipes finegoldii

Synonyms

EC Number Synonyms Comment Organism
1.3.1.9 FabI
-
 Alistipes finegoldii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.3.1.9 additional information
-
 presence of NADH increases the stability of FabI to thermal denaturation by 7 degrees Alistipes finegoldii

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.9 additional information no cofactor: NADPH Alistipes finegoldii
1.3.1.9 NADH protein exhibits high-affinity, cooperative binding to NADH. Presence of NADH increases the stability of FabI to thermal denaturation by 7 degrees Alistipes finegoldii

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.3.1.9 0.00176
-
 pH 8, 30°C Alistipes finegoldii triclosan