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Literature summary extracted from

  • Guo, X.; Zhang, M.; Cao, M.; Zhang, W.; Kang, Z.; Xu, P.; Ma, C.; Gao, C.
    D-2-Hydroxyglutarate dehydrogenase plays a dual role in L-serine biosynthesis and D-malate utilization in the bacterium Pseudomonas stutzeri (2018), J. Biol. Chem., 293, 15513-15523 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.99.39 expression in Escherichia coli Pseudomonas stutzeri

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.99.39 0.00471
-
 electron transfer flavoprotein pH 7.4, 30°C Pseudomonas stutzeri
1.1.99.39 0.17
-
 (R)-2-hydroxyglutarate pH 7.4, 30°C Pseudomonas stutzeri

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.99.39 (R)-2-hydroxyglutarate + electron transfer flavoprotein Pseudomonas stutzeri
-
 2-oxoglutarate + reduced electron transfer flavoprotein
-
 ?
1.1.99.39 (R)-2-hydroxyglutarate + electron transfer flavoprotein Pseudomonas stutzeri A1501
-
 2-oxoglutarate + reduced electron transfer flavoprotein
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.99.39 Pseudomonas stutzeri
-
-
-
1.1.99.39 Pseudomonas stutzeri A1501
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.99.39 nickel-affinity chromatography Pseudomonas stutzeri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.99.39 (R)-2-hydroxyglutarate + electron transfer flavoprotein
-
 Pseudomonas stutzeri 2-oxoglutarate + reduced electron transfer flavoprotein
-
 ?
1.1.99.39 (R)-2-hydroxyglutarate + electron transfer flavoprotein
-
 Pseudomonas stutzeri A1501 2-oxoglutarate + reduced electron transfer flavoprotein
-
 ?
1.1.99.39 (R)-2-hydroxyglutarate + phenazinemethosulfate
-
 Pseudomonas stutzeri 2-oxoglutarate + reduced phenazinemethosulfate
-
 ?
1.1.99.39 (R)-2-hydroxyglutarate + phenazinemethosulfate
-
 Pseudomonas stutzeri A1501 2-oxoglutarate + reduced phenazinemethosulfate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.99.39 D-2-HG dehydrogenase
-
 Pseudomonas stutzeri
1.1.99.39 D2HGDH
-
 Pseudomonas stutzeri

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.99.39 4.62
-
 electron transfer flavoprotein pH 7.4, 30°C Pseudomonas stutzeri
1.1.99.39 7.9
-
 (R)-2-hydroxyglutarate pH 7.4, 30°C Pseudomonas stutzeri

General Information

EC Number General Information Comment Organism
1.1.99.39 physiological function the enzyme plays a dual role in L-serine biosynthesis and D-malate utilization Pseudomonas stutzeri

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.99.39 45.39
-
 (R)-2-hydroxyglutarate pH 7.4, 30°C Pseudomonas stutzeri
1.1.99.39 980.82
-
 electron transfer flavoprotein pH 7.4, 30°C Pseudomonas stutzeri