EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.8.4.2 | 0.00087 | - |
glutathionylated glyceraldehyde-3-phosphate dehydrogenase | S0.5 value, pH 7.9, temperature not specified in the publication | Chlamydomonas reinhardtii | |
1.8.4.2 | 0.00454 | - |
glutathionylated glyceraldehyde-3-phosphate dehydrogenase | S0.5 value, pH 7.9, temperature not specified in the publication | Chlamydomonas reinhardtii | |
1.8.4.2 | 2.65 | - |
2-mercaptoethanol glutathione disulfide | pH 7.9, temperature not specified in the publication | Chlamydomonas reinhardtii | |
1.8.5.1 | 0.39 | - |
dehydroascorbate | pH 7.0, temperature not specified in the publication | Chlamydomonas reinhardtii |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.8.4.2 | chloroplast | - |
Chlamydomonas reinhardtii | 9507 | - |
1.8.4.2 | cytosol | - |
Chlamydomonas reinhardtii | 5829 | - |
1.8.5.1 | cytosol | - |
Chlamydomonas reinhardtii | 5829 | - |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.8.4.2 | Chlamydomonas reinhardtii | A8JH05 | isoform GRX3, CGFS type enzyme | - |
1.8.4.2 | Chlamydomonas reinhardtii | A8JHA9 | isoform GRX1 | - |
1.8.5.1 | Chlamydomonas reinhardtii | A8JHA9 | isoform GRX1 | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.8.4.2 | 2 glutathione + insulin disulfide | - |
Chlamydomonas reinhardtii | glutathione disulfide + insulin | - |
? | |
1.8.4.2 | 2-mercaptoethanol glutathione disulfide + glutathione | - |
Chlamydomonas reinhardtii | 2-mercaptoethanol disulfide + glutathione disulfide | - |
? | |
1.8.4.2 | dithiothreitol + insulin disulfide | - |
Chlamydomonas reinhardtii | dithiothreitol disulfide + insulin | - |
? | |
1.8.4.2 | glutathionylated glyceraldehyde-3-phosphate dehydrogenase + glutathione | substrate is glutathionylated A4-GAPDH. A4-GAPDH activity is reversibly inhibited by glutathionylation | Chlamydomonas reinhardtii | glutathione disulfide + glyceraldehyde-3-phosphate dehydrogenase | - |
? | |
1.8.4.2 | glutathionylated glyceraldehyde-3-phosphate dehydrogenase + reduced GRX3 | substrate is glutathionylated A4-GAPDH. A4-GAPDH activity is reversibly inhibited by glutathionylation | Chlamydomonas reinhardtii | glutathione + glyceraldehyde-3-phosphate dehydrogenase + oxidized GRX3 | - |
? | |
1.8.4.2 | additional information | GRX1is a typical CPYC-type GRX, which is reduced by GSH and exhibits disulfide reductase, dehydroascorbate reductase, and deglutathionylation activities | Chlamydomonas reinhardtii | ? | - |
- |
|
1.8.4.2 | additional information | no substrate: insulin disulfide, 2-mercaptoethanol glutathione disulfide | Chlamydomonas reinhardtii | ? | - |
- |
|
1.8.5.1 | 2 glutathione + dehydroascorbate | - |
Chlamydomonas reinhardtii | glutathione disulfide + ascorbate | - |
? | |
1.8.5.1 | additional information | GRX1is a typical CPYC-type GRX, which is reduced by GSH and exhibits disulfide reductase, dehydroascorbate reductase, and deglutathionylation activities | Chlamydomonas reinhardtii | ? | - |
- |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.8.4.2 | GRX1 | - |
Chlamydomonas reinhardtii |
1.8.4.2 | GRX3 | - |
Chlamydomonas reinhardtii |
1.8.5.1 | GRX1 | - |
Chlamydomonas reinhardtii |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.8.4.2 | 161.6 | - |
2-mercaptoethanol glutathione disulfide | pH 7.9, temperature not specified in the publication | Chlamydomonas reinhardtii | |
1.8.5.1 | 1.67 | - |
dehydroascorbate | pH 7.0, temperature not specified in the publication | Chlamydomonas reinhardtii |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.8.4.2 | physiological function | CGFS-type GRX is not reduced by GSH and has an atypically low redox potential (-323 mV at pH 7.9). GRX3 can be reduced in the light by photoreduced ferredoxin and ferredoxin-thioredoxin reductase | Chlamydomonas reinhardtii |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.8.4.2 | 61 | - |
2-mercaptoethanol glutathione disulfide | pH 7.9, temperature not specified in the publication | Chlamydomonas reinhardtii | |
1.8.5.1 | 4.3 | - |
dehydroascorbate | pH 7.0, temperature not specified in the publication | Chlamydomonas reinhardtii |