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Literature summary extracted from

  • Zaffagnini, M.; Michelet, L.; Massot, V.; Trost, P.; Lemaire, S.D.
    Biochemical characterization of glutaredoxins from Chlamydomonas reinhardtii reveals the unique properties of a chloroplastic CGFS-type glutaredoxin (2008), J. Biol. Chem., 283, 8868-8876 .
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.4.2 0.00087
-
glutathionylated glyceraldehyde-3-phosphate dehydrogenase S0.5 value, pH 7.9, temperature not specified in the publication Chlamydomonas reinhardtii
1.8.4.2 0.00454
-
glutathionylated glyceraldehyde-3-phosphate dehydrogenase S0.5 value, pH 7.9, temperature not specified in the publication Chlamydomonas reinhardtii
1.8.4.2 2.65
-
2-mercaptoethanol glutathione disulfide pH 7.9, temperature not specified in the publication Chlamydomonas reinhardtii
1.8.5.1 0.39
-
dehydroascorbate pH 7.0, temperature not specified in the publication Chlamydomonas reinhardtii

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.8.4.2 chloroplast
-
Chlamydomonas reinhardtii 9507
-
1.8.4.2 cytosol
-
Chlamydomonas reinhardtii 5829
-
1.8.5.1 cytosol
-
Chlamydomonas reinhardtii 5829
-

Organism

EC Number Organism UniProt Comment Textmining
1.8.4.2 Chlamydomonas reinhardtii A8JH05 isoform GRX3, CGFS type enzyme
-
1.8.4.2 Chlamydomonas reinhardtii A8JHA9 isoform GRX1
-
1.8.5.1 Chlamydomonas reinhardtii A8JHA9 isoform GRX1
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.4.2 2 glutathione + insulin disulfide
-
Chlamydomonas reinhardtii glutathione disulfide + insulin
-
?
1.8.4.2 2-mercaptoethanol glutathione disulfide + glutathione
-
Chlamydomonas reinhardtii 2-mercaptoethanol disulfide + glutathione disulfide
-
?
1.8.4.2 dithiothreitol + insulin disulfide
-
Chlamydomonas reinhardtii dithiothreitol disulfide + insulin
-
?
1.8.4.2 glutathionylated glyceraldehyde-3-phosphate dehydrogenase + glutathione substrate is glutathionylated A4-GAPDH. A4-GAPDH activity is reversibly inhibited by glutathionylation Chlamydomonas reinhardtii glutathione disulfide + glyceraldehyde-3-phosphate dehydrogenase
-
?
1.8.4.2 glutathionylated glyceraldehyde-3-phosphate dehydrogenase + reduced GRX3 substrate is glutathionylated A4-GAPDH. A4-GAPDH activity is reversibly inhibited by glutathionylation Chlamydomonas reinhardtii glutathione + glyceraldehyde-3-phosphate dehydrogenase + oxidized GRX3
-
?
1.8.4.2 additional information GRX1is a typical CPYC-type GRX, which is reduced by GSH and exhibits disulfide reductase, dehydroascorbate reductase, and deglutathionylation activities Chlamydomonas reinhardtii ?
-
-
1.8.4.2 additional information no substrate: insulin disulfide, 2-mercaptoethanol glutathione disulfide Chlamydomonas reinhardtii ?
-
-
1.8.5.1 2 glutathione + dehydroascorbate
-
Chlamydomonas reinhardtii glutathione disulfide + ascorbate
-
?
1.8.5.1 additional information GRX1is a typical CPYC-type GRX, which is reduced by GSH and exhibits disulfide reductase, dehydroascorbate reductase, and deglutathionylation activities Chlamydomonas reinhardtii ?
-
-

Synonyms

EC Number Synonyms Comment Organism
1.8.4.2 GRX1
-
Chlamydomonas reinhardtii
1.8.4.2 GRX3
-
Chlamydomonas reinhardtii
1.8.5.1 GRX1
-
Chlamydomonas reinhardtii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.8.4.2 161.6
-
2-mercaptoethanol glutathione disulfide pH 7.9, temperature not specified in the publication Chlamydomonas reinhardtii
1.8.5.1 1.67
-
dehydroascorbate pH 7.0, temperature not specified in the publication Chlamydomonas reinhardtii

General Information

EC Number General Information Comment Organism
1.8.4.2 physiological function CGFS-type GRX is not reduced by GSH and has an atypically low redox potential (-323 mV at pH 7.9). GRX3 can be reduced in the light by photoreduced ferredoxin and ferredoxin-thioredoxin reductase Chlamydomonas reinhardtii

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.8.4.2 61
-
2-mercaptoethanol glutathione disulfide pH 7.9, temperature not specified in the publication Chlamydomonas reinhardtii
1.8.5.1 4.3
-
dehydroascorbate pH 7.0, temperature not specified in the publication Chlamydomonas reinhardtii