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Literature summary extracted from

  • Rachadech, W.; Kato, Y.; Abou El-Magd, R.M.; Shishido, Y.; Kim, S.H.; Sogabe, H.; Maita, N.; Yorita, K.; Fukui, K.
    P219L substitution in human D-amino acid oxidase impacts the ligand binding and catalytic efficiency (2020), J. Biochem., 168, 557-567 .
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.4.3.3 structure of mutant P219L in complex with FAD and benzoate at 2.25 A resolution displays conformational changes of the active site and lid. The distances between the H-bond-forming atoms of arginine 283 and benzoate and the relative position between the aromatic rings of tyrosine 224 and benzoate are changed in the P219L complex Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
1.4.3.3 P219L mutation to corresponding residue of porcine DAO. The turnover numbers (kcat) of P219L are unchanged, but its Km values are decreased compared with wild-type, leading to an increase in the catalytic efficiency (kcat/Km). Benzoate inhibits P219L with lower Ki value Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.3.3 benzoate competitive Homo sapiens
1.4.3.3 D-alanine
-
Homo sapiens
1.4.3.3 D-proline
-
Homo sapiens
1.4.3.3 D-serine
-
Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.3.3 0.47
-
D-alanine mutant P219L, pH 8.0, 25°C Homo sapiens
1.4.3.3 0.73
-
D-proline mutant P219L, pH 8.0, 25°C Homo sapiens
1.4.3.3 0.75
-
D-alanine wild-type, pH 8.0, 25°C Homo sapiens
1.4.3.3 1.23
-
D-proline wild-type, pH 8.0, 25°C Homo sapiens
1.4.3.3 1.36
-
D-serine mutant P219L, pH 8.0, 25°C Homo sapiens
1.4.3.3 4.29
-
D-serine wild-type, pH 8.0, 25°C Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.3 Homo sapiens P14920
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.3.3 partial purification by heat treatment at 53°C Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.3 D-alanine + H2O + O2
-
Homo sapiens pyruvate + H2O2 + NH3
-
?
1.4.3.3 D-proline + H2O + O2
-
Homo sapiens 2-oxopentanoate + NH3 + H2O2
-
?
1.4.3.3 D-serine + H2O + O2
-
Homo sapiens 3-hydroxypyruvate + NH3 + H2O2
-
?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.4.3.3 6.08
-
D-serine mutant P219L, pH 8.0, 25°C Homo sapiens
1.4.3.3 6.55
-
D-serine wild-type, pH 8.0, 25°C Homo sapiens
1.4.3.3 8.3
-
D-alanine mutant P219L, pH 8.0, 25°C Homo sapiens
1.4.3.3 9.48
-
D-alanine wild-type, pH 8.0, 25°C Homo sapiens
1.4.3.3 26.57
-
D-proline wild-type, pH 8.0, 25°C Homo sapiens
1.4.3.3 29.53
-
D-proline mutant P219L, pH 8.0, 25°C Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.3.3 FAD
-
Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.4.3.3 0.0007
-
D-alanine mutant P219L, pH 8.0, 25°C Homo sapiens
1.4.3.3 0.0007
-
D-serine mutant P219L, pH 8.0, 25°C Homo sapiens
1.4.3.3 0.0009
-
D-proline mutant P219L, pH 8.0, 25°C Homo sapiens
1.4.3.3 0.0012
-
D-alanine wild-type, pH 8.0, 25°C Homo sapiens
1.4.3.3 0.0017
-
D-proline wild-type, pH 8.0, 25°C Homo sapiens
1.4.3.3 0.002
-
D-serine wild-type, pH 8.0, 25°C Homo sapiens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.4.3.3 1.53
-
D-serine wild-type, pH 8.0, 25°C Homo sapiens
1.4.3.3 4.67
-
D-serine mutant P219L, pH 8.0, 25°C Homo sapiens
1.4.3.3 13.38
-
D-alanine wild-type, pH 8.0, 25°C Homo sapiens
1.4.3.3 18.52
-
D-alanine mutant P219L, pH 8.0, 25°C Homo sapiens
1.4.3.3 21.93
-
D-proline wild-type, pH 8.0, 25°C Homo sapiens
1.4.3.3 41.47
-
D-proline mutant P219L, pH 8.0, 25°C Homo sapiens