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Literature summary extracted from

  • Kato, T.; Shirakawa, Y.; Takegawa, K.; Kimura, Y.
    Functional analysis of conserved motifs in a bacterial tyrosine kinase, BtkB, from Myxococcus xanthus (2015), J. Biochem., 158, 385-392 .
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
2.7.10.3 additional information a deletion mutant lacking the predicted activator domain (amino acids 444-464) loses autophosphorylation and poly(Glu, Tyr) kinase activities. A cytoplasmic fragment of BtkB (Ser444-Ser710) carrying replacments of all C-terminal tyrosine residues with phenylalanines, does not undergo autophosphorylation. This mutation does not significantly affect poly(Glu, Tyr) kinase activity. A cytoplasmic fragment of BtkB carrying mutations of the conserved motif ExxRxxR of BY kinases which is involved in the self-association of catalytic domains, leads to loss of autophosphorylation and poly(Glu, Tyr) kinase activities Myxococcus xanthus
2.7.10.3 R550Y mutation reduces autophosphorylation activity by about 95% and the poly(Glu, Tyr) activity by about 91% Myxococcus xanthus
2.7.10.3 Y686F/Y690F/Y693F/Y696F/Y699F complete loss of autophosphorylation with only slightly reduced poly(Glu, Tyr) kinase activities Myxococcus xanthus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.10.3 4-amino-5-(4-chlorophenyl)-7-(t-butyl)pyrazolo[3,4-d]pyrimidine 0.2 mM, 64% inhibition Myxococcus xanthus
2.7.10.3 genistein 0.2 mM, 20% inhibition Myxococcus xanthus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.10.3 additional information
-
 L-tyrosine in poly(Glu,Tyr) Km value 0.3 mg/ml, cytoplasmic fragment of BtkB (Ser444-Ser710), pH 7, 37°C Myxococcus xanthus
2.7.10.3 additional information
-
 L-tyrosine in poly(Glu,Tyr) Km value 0.375 mg/ml, mutant Y686F/Y690F/Y693F/Y696F/Y699F, pH 7, 37°C Myxococcus xanthus
2.7.10.3 additional information
-
 L-tyrosine in poly(Glu,Tyr) Km value 1.35 mg/ml, mutant R550Y, pH 7, 37°C Myxococcus xanthus

Organism

EC Number Organism UniProt Comment Textmining
2.7.10.3 Myxococcus xanthus Q1DDI7 isoform BtkB
-
2.7.10.3 Myxococcus xanthus DK1622 Q1DDI7 isoform BtkB
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.10.3 ATP + L-tyrosine in poly(Glu,Tyr) poly(Glu, Tyr; 4:1), used as universal substrate for protein tyrosine kinases Myxococcus xanthus ADP + H+ + O-phospho-L-tyrosyl-poly(Glu,Tyr)
-
 ?
2.7.10.3 ATP + L-tyrosine in poly(Glu,Tyr) poly(Glu, Tyr; 4:1), used as universal substrate for protein tyrosine kinases Myxococcus xanthus DK1622 ADP + H+ + O-phospho-L-tyrosyl-poly(Glu,Tyr)
-
 ?
2.7.10.3 ATP + L-tyrosyl-[BtkB]
-
 Myxococcus xanthus ADP + H+ + O-phospho-L-tyrosyl-[BtkB]
-
 ?
2.7.10.3 ATP + L-tyrosyl-[BtkB]
-
 Myxococcus xanthus DK1622 ADP + H+ + O-phospho-L-tyrosyl-[BtkB]
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.10.3 0.00002
-
 L-tyrosine in poly(Glu,Tyr) mutant R550Y, pH 7, 37°C Myxococcus xanthus
2.7.10.3 0.0002
-
 L-tyrosine in poly(Glu,Tyr) mutant Y686F/Y690F/Y693F/Y696F/Y699F, pH 7, 37°C Myxococcus xanthus
2.7.10.3 0.0003
-
 L-tyrosine in poly(Glu,Tyr) cytoplasmic fragment of BtkB (Ser444-Ser710), pH 7, 37°C Myxococcus xanthus