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Literature summary extracted from

  • Vogt, M.; Schuehle, K.; Koelzer, S.; Peschke, P.; Chowdhury, N.; Kleinsorge, D.; Buckel, W.; Essen, L.; Heider, J.
    Structural and functional characterization of an electron transfer flavoprotein involved in toluene degradation in strictly anaerobic bacteria (2021), J. Bacteriol., 201, e00326-19 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.8.3 gene bbsG, recombinant overexpression of the enzyme in Escherichia coli Geobacter metallireducens
1.3.8.3 gene bbsG, recombinant overexpression of the enzyme in Escherichia coli Thauera aromatica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.8.3 0.098
-
 (R)-2-benzylsuccinyl-CoA forward reaction, recombinant enzyme, pH 6.4, 50°C Geobacter metallireducens
1.3.8.3 0.14
-
 (R)-2-benzylsuccinyl-CoA forward reaction, recombinant enzyme, pH 6.4, 50°C Thauera aromatica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.8.3 (R)-2-benzylsuccinyl-CoA + electron-transfer flavoprotein Geobacter metallireducens
-
 (E)-2-benzylidenesuccinyl-CoA + reduced electron-transfer flavoprotein
-
 r
1.3.8.3 (R)-2-benzylsuccinyl-CoA + electron-transfer flavoprotein Desulfobacula toluolica
-
 (E)-2-benzylidenesuccinyl-CoA + reduced electron-transfer flavoprotein
-
 ?
1.3.8.3 (R)-2-benzylsuccinyl-CoA + electron-transfer flavoprotein Aromatoleum aromaticum
-
 (E)-2-benzylidenesuccinyl-CoA + reduced electron-transfer flavoprotein
-
 ?
1.3.8.3 (R)-2-benzylsuccinyl-CoA + electron-transfer flavoprotein Thauera aromatica
-
 (E)-2-benzylidenesuccinyl-CoA + reduced electron-transfer flavoprotein
-
 r
1.3.8.3 (R)-2-benzylsuccinyl-CoA + electron-transfer flavoprotein Geobacter metallireducens GS-15
-
 (E)-2-benzylidenesuccinyl-CoA + reduced electron-transfer flavoprotein
-
 r
1.3.8.3 (R)-2-benzylsuccinyl-CoA + electron-transfer flavoprotein Geobacter metallireducens DSM 7210
-
 (E)-2-benzylidenesuccinyl-CoA + reduced electron-transfer flavoprotein
-
 r
1.3.8.3 (R)-2-benzylsuccinyl-CoA + electron-transfer flavoprotein Geobacter metallireducens ATCC 53774
-
 (E)-2-benzylidenesuccinyl-CoA + reduced electron-transfer flavoprotein
-
 r
1.3.8.3 (R)-2-benzylsuccinyl-CoA + electron-transfer flavoprotein Desulfobacula toluolica DSM 7467
-
 (E)-2-benzylidenesuccinyl-CoA + reduced electron-transfer flavoprotein
-
 ?
1.3.8.3 (R)-2-benzylsuccinyl-CoA + electron-transfer flavoprotein Aromatoleum aromaticum EbN1
-
 (E)-2-benzylidenesuccinyl-CoA + reduced electron-transfer flavoprotein
-
 ?
1.3.8.3 (R)-2-benzylsuccinyl-CoA + electron-transfer flavoprotein Desulfobacula toluolica Tol2
-
 (E)-2-benzylidenesuccinyl-CoA + reduced electron-transfer flavoprotein
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.8.3 Aromatoleum aromaticum Q5P690 Azoarcus sp. EbN1
-
1.3.8.3 Aromatoleum aromaticum EbN1 Q5P690 Azoarcus sp. EbN1
-
1.3.8.3 Desulfobacula toluolica K0NF66
-
-
1.3.8.3 Desulfobacula toluolica DSM 7467 K0NF66
-
-
1.3.8.3 Desulfobacula toluolica Tol2 K0NF66
-
-
1.3.8.3 Geobacter metallireducens Q39VG7
-
-
1.3.8.3 Geobacter metallireducens ATCC 53774 Q39VG7
-
-
1.3.8.3 Geobacter metallireducens DSM 7210 Q39VG7
-
-
1.3.8.3 Geobacter metallireducens GS-15 Q39VG7
-
-
1.3.8.3 Thauera aromatica Q9KJE8
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.8.3 4.4
-
 reverse reaction, recombinant enzyme, pH 6.4, 50°C Geobacter metallireducens
1.3.8.3 7
-
 reverse reaction, recombinant enzyme, pH 6.4, 50°C Thauera aromatica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.8.3 (R)-2-benzylsuccinyl-CoA + electron-transfer flavoprotein
-
 Geobacter metallireducens (E)-2-benzylidenesuccinyl-CoA + reduced electron-transfer flavoprotein
-
 r
1.3.8.3 (R)-2-benzylsuccinyl-CoA + electron-transfer flavoprotein
-
 Desulfobacula toluolica (E)-2-benzylidenesuccinyl-CoA + reduced electron-transfer flavoprotein
-
 ?
1.3.8.3 (R)-2-benzylsuccinyl-CoA + electron-transfer flavoprotein
-
 Aromatoleum aromaticum (E)-2-benzylidenesuccinyl-CoA + reduced electron-transfer flavoprotein
-
 ?
1.3.8.3 (R)-2-benzylsuccinyl-CoA + electron-transfer flavoprotein
-
 Thauera aromatica (E)-2-benzylidenesuccinyl-CoA + reduced electron-transfer flavoprotein
-
 r
1.3.8.3 (R)-2-benzylsuccinyl-CoA + electron-transfer flavoprotein
-
 Geobacter metallireducens GS-15 (E)-2-benzylidenesuccinyl-CoA + reduced electron-transfer flavoprotein
-
 r
1.3.8.3 (R)-2-benzylsuccinyl-CoA + electron-transfer flavoprotein
-
 Geobacter metallireducens DSM 7210 (E)-2-benzylidenesuccinyl-CoA + reduced electron-transfer flavoprotein
-
 r
1.3.8.3 (R)-2-benzylsuccinyl-CoA + electron-transfer flavoprotein
-
 Geobacter metallireducens ATCC 53774 (E)-2-benzylidenesuccinyl-CoA + reduced electron-transfer flavoprotein
-
 r
1.3.8.3 (R)-2-benzylsuccinyl-CoA + electron-transfer flavoprotein
-
 Desulfobacula toluolica DSM 7467 (E)-2-benzylidenesuccinyl-CoA + reduced electron-transfer flavoprotein
-
 ?
1.3.8.3 (R)-2-benzylsuccinyl-CoA + electron-transfer flavoprotein
-
 Aromatoleum aromaticum EbN1 (E)-2-benzylidenesuccinyl-CoA + reduced electron-transfer flavoprotein
-
 ?
1.3.8.3 (R)-2-benzylsuccinyl-CoA + electron-transfer flavoprotein
-
 Desulfobacula toluolica Tol2 (E)-2-benzylidenesuccinyl-CoA + reduced electron-transfer flavoprotein
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.8.3 ? x * 45580, recombinant enzyme, SDS-PAGE Thauera aromatica
1.3.8.3 ? x * 46360, recombinant enzyme, SDS-PAGE Geobacter metallireducens

Synonyms

EC Number Synonyms Comment Organism
1.3.8.3 bbgS
-
 Geobacter metallireducens
1.3.8.3 bbgS
-
 Desulfobacula toluolica
1.3.8.3 BbsG
-
 Geobacter metallireducens
1.3.8.3 BbsG
-
 Desulfobacula toluolica
1.3.8.3 BbsG
-
 Aromatoleum aromaticum
1.3.8.3 BbsG
-
 Thauera aromatica

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.8.3 50
-
 recombinant enzyme Geobacter metallireducens
1.3.8.3 50
-
 recombinant enzyme Thauera aromatica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.8.3 6.4
-
 recombinant enzyme Geobacter metallireducens
1.3.8.3 8
-
 recombinant enzyme Thauera aromatica

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.8.3 electron transferring flavoprotein ETF, structural and functional characterization of the electron transfer flavoprotein involved in toluene degradation in the strictly anaerobic bacteria, binding kinetics analysis, overview. Unrooted phylogenetic tree representing evolutionary branches of the ETFAB family proteins Thauera aromatica
1.3.8.3 electron transferring flavoprotein structural and functional characterization of the electron transfer flavoprotein involved in toluene degradation in the strictly anaerobic bacteria, DNA and amino acid sequence determination and analysis, UniProt IDs: K0NC26 (etfA1) and K0NCE4 (etfB1). Unrooted phylogenetic tree representing evolutionary branches of the ETFAB family proteins, overview Desulfobacula toluolica
1.3.8.3 electron transferring flavoprotein structural and functional characterization of the electron transfer flavoprotein involved in toluene degradation in the strictly anaerobic bacteria, DNA and amino acid sequence determination and analysis, UniProt IDs: Q39VG4 (etfA) and Q39VG5 (etfB), binding kinetics analysis, overview. ETF crystal structure determination and analysis. Unrooted phylogenetic tree representing evolutionary branches of the ETFAB family proteins Geobacter metallireducens
1.3.8.3 electron transferring flavoprotein structural and functional characterization of the electron transfer flavoprotein involved in toluene degradation in the strictly anaerobic bacteria, DNA and amino acid sequence determination and analysis, UniProt IDs: Q5NYM0 (etfA) and Q5NYL9 (etfB). Unrooted phylogenetic tree representing evolutionary branches of the ETFAB family proteins, overview Aromatoleum aromaticum
1.3.8.3 FAD
-
 Desulfobacula toluolica
1.3.8.3 FAD
-
 Aromatoleum aromaticum
1.3.8.3 FAD 0.92 mol/mol enzyme Thauera aromatica
1.3.8.3 FAD 0.99 mol/mol enzyme Geobacter metallireducens
1.3.8.3 additional information in contrast to ETF, reduction of BbsG with dithionite leads directly to the fully reduced form without transient semiquinone formation Geobacter metallireducens
1.3.8.3 additional information in contrast to ETF, reduction of BbsG with dithionite leads directly to the fully reduced form without transient semiquinone formation Thauera aromatica