Literature summary extracted from

Xu, D.; Wu, M.; Li, X.; Xia, M.; Liu, D.; Dai, Y.; Yu, Q.; Wu, B.; Wang, D.
Cloning, prokaryotic expression and function of the Bufo bufo gargarizans 3beta-hydroxysteroid dehydrogenase (3betaHSD) gene (2018), Int. J. Biol. Macromol., 120, 673-682 .

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
1.1.1.145	cloned from the ear-side gland, expression in Escherichia coli	Bufo gargarizans

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
1.1.1.145	0.2313	-	pregnenolone	pH 7.8, 30°C	Bufo gargarizans
1.1.1.145	0.2419	-	dehydroepiandrosterone	pH 7.8, 30°C	Bufo gargarizans

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
1.1.1.145	42800	-	calculated from sequence	Bufo gargarizans

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.1.1.145	Bufo gargarizans	A0A345GNZ1	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
1.1.1.145	-	Bufo gargarizans

Source Tissue

EC Number	Source Tissue	Comment	Organism	Textmining
1.1.1.145	liver	expression of 3beta-hydroxysteroid dehydrogenase (3betaHSD) in ear-side gland is nearly 20times higher than that in liver	Bufo gargarizans	-
1.1.1.145	parotid gland	expression of 3beta-hydroxysteroid dehydrogenase (3betaHSD) in ear-side gland is nearly 20times higher than that in liver	Bufo gargarizans	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1.1.1.145	dehydroepiandrosterone + NAD+	when the coenzyme is NADP+ the yield decreases significantly	Bufo gargarizans	androstenedione + NADH + H+	-	?
1.1.1.145	pregnenolone + NAD+	NAD+ is more suitable coenzyme than NADP+	Bufo gargarizans	progesterone + NADH + H+	-	?

Synonyms

EC Number	Synonyms	Comment	Organism
1.1.1.145	3beta-hydroxysteroid dehydrogenase	-	Bufo gargarizans
1.1.1.145	3betaHSD	-	Bufo gargarizans

Temperature Optimum [°C]

EC Number	Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
1.1.1.145	40	-	recombinant enzyme	Bufo gargarizans

Temperature Range [°C]

EC Number	Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
1.1.1.145	30	50	30°C: about 30% of maximal activity, 30°C: about 35% of maximal activity	Bufo gargarizans

Turnover Number [1/s]

EC Number	Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
1.1.1.145	0.64	-	dehydroepiandrosterone	pH 7.8, 30°C	Bufo gargarizans
1.1.1.145	2.8	-	pregnenolone	pH 7.8, 30°C	Bufo gargarizans

pH Optimum

EC Number	pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
1.1.1.145	8.5	-	recombinant enzyme	Bufo gargarizans

pH Range

EC Number	pH Minimum	pH Maximum	Comment	Organism
1.1.1.145	6.5	10	pH 6.5: about 55% of maximal activity, pH 10.0: about 45% of maximal activity	Bufo gargarizans

Cofactor

EC Number	Cofactor	Comment	Organism	Structure
1.1.1.145	NAD+	optimum coenzyme concentration is 1.5 mmol/l	Bufo gargarizans

pI Value

EC Number	Organism	Comment	pI Value Maximum	pI Value
1.1.1.145	Bufo gargarizans	calculated from sequence	-	8.68

General Information

EC Number	General Information	Comment	Organism
1.1.1.145	metabolism	the enzyme catalyzes an essential step in the biosynthesis of steroid such as progesterone, estrogens and androgens in steroidogenic tissues. It is probably an important enzyme involved in the biosynthesis of bufadienolides	Bufo gargarizans

kcat/KM [mM/s]

EC Number	kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
1.1.1.145	2.65	-	dehydroepiandrosterone	pH 7.8, 30°C	Bufo gargarizans
1.1.1.145	12.12	-	pregnenolone	pH 7.8, 30°C	Bufo gargarizans

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Release 2023.1 (January 2023)