EC Number | Cloned (Comment) | Organism |
---|---|---|
1.3.99.31 | gene crtI, sequence comparisons and phylogenetic analysis, quantitative real-time reverse transcription PCR enzyme expression analysis, recombinant expression in Escherichia coli strain SIG10 | Puccinia graminis f. sp. tritici |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.99.31 | 15-cis-phytoene + acceptor | Puccinia graminis f. sp. tritici | - |
all-trans-phytofluene + reduced acceptor | - |
? | |
1.3.99.31 | 15-cis-phytoene + acceptor | Puccinia graminis f. sp. tritici race SCCL | - |
all-trans-phytofluene + reduced acceptor | - |
? | |
1.3.99.31 | 15-cis-phytoene + acceptor | Puccinia graminis f. sp. tritici CRL 75-36-700-3 | - |
all-trans-phytofluene + reduced acceptor | - |
? | |
1.3.99.31 | all-trans-neurosporene + acceptor | Puccinia graminis f. sp. tritici | - |
all-trans-lycopene + reduced acceptor | - |
? | |
1.3.99.31 | all-trans-neurosporene + acceptor | Puccinia graminis f. sp. tritici race SCCL | - |
all-trans-lycopene + reduced acceptor | - |
? | |
1.3.99.31 | all-trans-neurosporene + acceptor | Puccinia graminis f. sp. tritici CRL 75-36-700-3 | - |
all-trans-lycopene + reduced acceptor | - |
? | |
1.3.99.31 | all-trans-phytofluene + acceptor | Puccinia graminis f. sp. tritici | - |
all-trans-zeta-carotene + reduced acceptor | - |
? | |
1.3.99.31 | all-trans-phytofluene + acceptor | Puccinia graminis f. sp. tritici race SCCL | - |
all-trans-zeta-carotene + reduced acceptor | - |
? | |
1.3.99.31 | all-trans-phytofluene + acceptor | Puccinia graminis f. sp. tritici CRL 75-36-700-3 | - |
all-trans-zeta-carotene + reduced acceptor | - |
? | |
1.3.99.31 | all-trans-zeta-carotene + acceptor | Puccinia graminis f. sp. tritici | - |
all-trans-neurosporene + reduced acceptor | - |
? | |
1.3.99.31 | all-trans-zeta-carotene + acceptor | Puccinia graminis f. sp. tritici race SCCL | - |
all-trans-neurosporene + reduced acceptor | - |
? | |
1.3.99.31 | all-trans-zeta-carotene + acceptor | Puccinia graminis f. sp. tritici CRL 75-36-700-3 | - |
all-trans-neurosporene + reduced acceptor | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.3.99.31 | Puccinia graminis f. sp. tritici | E3L8P0 | - |
- |
1.3.99.31 | Puccinia graminis f. sp. tritici CRL 75-36-700-3 | E3L8P0 | - |
- |
1.3.99.31 | Puccinia graminis f. sp. tritici race SCCL | E3L8P0 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.99.31 | 15-cis-phytoene + acceptor | - |
Puccinia graminis f. sp. tritici | all-trans-phytofluene + reduced acceptor | - |
? | |
1.3.99.31 | 15-cis-phytoene + acceptor | - |
Puccinia graminis f. sp. tritici race SCCL | all-trans-phytofluene + reduced acceptor | - |
? | |
1.3.99.31 | 15-cis-phytoene + acceptor | - |
Puccinia graminis f. sp. tritici CRL 75-36-700-3 | all-trans-phytofluene + reduced acceptor | - |
? | |
1.3.99.31 | all-trans-neurosporene + acceptor | - |
Puccinia graminis f. sp. tritici | all-trans-lycopene + reduced acceptor | - |
? | |
1.3.99.31 | all-trans-neurosporene + acceptor | - |
Puccinia graminis f. sp. tritici race SCCL | all-trans-lycopene + reduced acceptor | - |
? | |
1.3.99.31 | all-trans-neurosporene + acceptor | - |
Puccinia graminis f. sp. tritici CRL 75-36-700-3 | all-trans-lycopene + reduced acceptor | - |
? | |
1.3.99.31 | all-trans-phytofluene + acceptor | - |
Puccinia graminis f. sp. tritici | all-trans-zeta-carotene + reduced acceptor | - |
? | |
1.3.99.31 | all-trans-phytofluene + acceptor | - |
Puccinia graminis f. sp. tritici race SCCL | all-trans-zeta-carotene + reduced acceptor | - |
? | |
1.3.99.31 | all-trans-phytofluene + acceptor | - |
Puccinia graminis f. sp. tritici CRL 75-36-700-3 | all-trans-zeta-carotene + reduced acceptor | - |
? | |
1.3.99.31 | all-trans-zeta-carotene + acceptor | - |
Puccinia graminis f. sp. tritici | all-trans-neurosporene + reduced acceptor | - |
? | |
1.3.99.31 | all-trans-zeta-carotene + acceptor | - |
Puccinia graminis f. sp. tritici race SCCL | all-trans-neurosporene + reduced acceptor | - |
? | |
1.3.99.31 | all-trans-zeta-carotene + acceptor | - |
Puccinia graminis f. sp. tritici CRL 75-36-700-3 | all-trans-neurosporene + reduced acceptor | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.3.99.31 | CrtI | - |
Puccinia graminis f. sp. tritici |
1.3.99.31 | PGT | - |
Puccinia graminis f. sp. tritici |
1.3.99.31 | PGTG_19044 | - |
Puccinia graminis f. sp. tritici |
1.3.99.31 | phytoene desaturase | - |
Puccinia graminis f. sp. tritici |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.3.99.31 | evolution | carotenoid biosynthesis and the evolution of carotenogenesis genes in rust fungi, phylogenetic analysis, detailed overview. A part of the carotenoid biosynthesis pathway in rust fungi is elucidated, with only two genes, CrtYB and CrtI, catalysing the reactions from geranyl-geranyl diphosphate (GGPP) to gamma-carotene. The CrtI gene encodes a phytoene desaturase carries out four successive desaturations of phytoene, through the intermediates phytofluene and neurosporene to lycopene. The CrtYB gene encodes a bifunctional lycopene cyclase/phytoene synthase, which catalyses the condensation of two GGPP into phytoene, as well as the cyclisation of the Psi-end of lycopene to form gamma-carotene | Puccinia graminis f. sp. tritici |
1.3.99.31 | malfunction | wild-type Pgt produces reddish-brown urediniospores with yellow cytoplasmic carotenoid pigment and brownish spore wall pigments. Ethyl methanesulfonate (EMS)-induced mutants produce chocolate-coloured and yellow-coloured urediniospores, the mutants lack detectable cytoplasmic carotenoid pigments and sporewall pigments, respectively, viability and infection efficiency of these mutants compared to the corresponding wild-type forms, overview. Reduced fitness is found in the two colour mutants compared to the wild-type isolate, supporting a virulence function for carotenoid pigments in rust fungi, phenotypes, overview | Puccinia graminis f. sp. tritici |
1.3.99.31 | metabolism | the carotenoid biosynthesis pathway of Pgt is deduced from the results of the carotenoid pigment analysis and the gene complementation assay: the first C40 carotenoid, phytoene, is synthesised via the condensation of two molecules of GGPP, catalysed by the bifunctional lycopene cyclase/phytoene synthase encoded by the CrtYB gene. Subsequently, phytoene undergoes four steps of desaturation catalysed by the phytoene desaturase encoded by CrtI, through the intermediates phytofluene and neurosporene to lycopene. The CrtYB bifunctional lycopene cyclase/phytoene synthase then catalyses the cyclisation of one Psi-end of lycopene into gamma-carotene. beta-Carotene is not detected in the gene complementation assay | Puccinia graminis f. sp. tritici |
1.3.99.31 | physiological function | the CrtI gene encodes a phytoene desaturase carries out four successive desaturations of phytoene, through the intermediates phytofluene and neurosporene to lycopene | Puccinia graminis f. sp. tritici |