Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Schenck, C.; Men, Y.; Maeda, H.
    Conserved molecular mechanism of TyrA dehydrogenase substrate specificity underlying alternative tyrosine biosynthetic pathways in plants and microbes (2017), Front. Mol. Biosci., 4, 73.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.13 expression in Escherichia coli Methanothrix harundinacea
1.3.1.79 expression in Escherichia coli Sediminispirochaeta smaragdinae

Protein Variants

EC Number Protein Variants Comment Organism
1.3.1.13 Q227E mutation introduces arogenate dehydrogenase activity Methanothrix harundinacea
1.3.1.79 D208N mutation introduces prephenate dehydrogenase activity Sediminispirochaeta smaragdinae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.1.13 0.38
-
 prephenate wild-type, pH 7.6, temperature not specified in the publication Methanothrix harundinacea
1.3.1.13 2.67
-
 prephenate mutant Q227E, pH 7.6, temperature not specified in the publication Methanothrix harundinacea
1.3.1.13 3.29
-
 L-arogenate mutant Q227E, pH 7.6, temperature not specified in the publication Methanothrix harundinacea
1.3.1.79 0.85
-
 L-arogenate mutant D208N, pH 7.6, temperature not specified in the publication Sediminispirochaeta smaragdinae
1.3.1.79 0.9
-
 L-arogenate wild-type, pH 7.6, temperature not specified in the publication Sediminispirochaeta smaragdinae
1.3.1.79 1.74
-
 prephenate mutant D208N, pH 7.6, temperature not specified in the publication Sediminispirochaeta smaragdinae

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.13 Methanothrix harundinacea A0A101IGG2
-
-
1.3.1.79 Sediminispirochaeta smaragdinae E1R5M5
-
-
1.3.1.79 Sediminispirochaeta smaragdinae DSM 11293 E1R5M5
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.13 L-arogenate + NADP+ no substrate of wild-type, reaction of EC 1.3.1.78 Methanothrix harundinacea L-tyrosine + NADPH + H+ + CO2
-
 ?
1.3.1.13 prephenate + NADP+
-
 Methanothrix harundinacea 4-hydroxyphenylpyruvate + NADPH + H+ + CO2
-
 ?
1.3.1.79 L-arogenate + NAD(P)+
-
 Sediminispirochaeta smaragdinae L-tyrosine + NAD(P)H + H+ + CO2
-
 ?
1.3.1.79 L-arogenate + NAD(P)+
-
 Sediminispirochaeta smaragdinae DSM 11293 L-tyrosine + NAD(P)H + H+ + CO2
-
 ?
1.3.1.79 prephenate + NAD(P)+ no substrate of wild-type, reaction of EC 1.3.1.12 Sediminispirochaeta smaragdinae 4-hydroxyphenylpyruvate + NAD(P)H + H+ + CO2
-
 ?
1.3.1.79 prephenate + NAD(P)+ no substrate of wild-type, reaction of EC 1.3.1.12 Sediminispirochaeta smaragdinae DSM 11293 4-hydroxyphenylpyruvate + NAD(P)H + H+ + CO2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.3.1.13 tyrA
-
 Methanothrix harundinacea
1.3.1.13 XD72_2094
-
 Methanothrix harundinacea
1.3.1.79 Spirs_1513
-
 Sediminispirochaeta smaragdinae
1.3.1.79 tyrA
-
 Sediminispirochaeta smaragdinae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.3.1.13 0.29
-
 prephenate mutant Q227E, pH 7.6, temperature not specified in the publication Methanothrix harundinacea
1.3.1.13 0.7
-
 L-arogenate mutant Q227E, pH 7.6, temperature not specified in the publication Methanothrix harundinacea
1.3.1.13 2.44
-
 prephenate wild-type, pH 7.6, temperature not specified in the publication Methanothrix harundinacea
1.3.1.79 0.45
-
 L-arogenate mutant D208N, pH 7.6, temperature not specified in the publication Sediminispirochaeta smaragdinae
1.3.1.79 0.86
-
 prephenate mutant D208N, pH 7.6, temperature not specified in the publication Sediminispirochaeta smaragdinae
1.3.1.79 3.44
-
 L-arogenate wild-type, pH 7.6, temperature not specified in the publication Sediminispirochaeta smaragdinae

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.13 NAD+ enzyme strongly prefers NADP+ over NAD+ Methanothrix harundinacea
1.3.1.13 NADP+ enzyme strongly prefers NADP+ over NAD+ Methanothrix harundinacea
1.3.1.79 NAD+ slight preference for NAD+ over NADP+ Sediminispirochaeta smaragdinae
1.3.1.79 NADP+ slight preference for NAD+ over NADP+ Sediminispirochaeta smaragdinae

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.3.1.13 0.11
-
 prephenate mutant Q227E, pH 7.6, temperature not specified in the publication Methanothrix harundinacea
1.3.1.13 0.21
-
 L-arogenate mutant Q227E, pH 7.6, temperature not specified in the publication Methanothrix harundinacea
1.3.1.13 6.44
-
 prephenate wild-type, pH 7.6, temperature not specified in the publication Methanothrix harundinacea
1.3.1.79 0.57
-
 L-arogenate mutant D208N, pH 7.6, temperature not specified in the publication Sediminispirochaeta smaragdinae
1.3.1.79 4
-
 L-arogenate wild-type, pH 7.6, temperature not specified in the publication Sediminispirochaeta smaragdinae