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Literature summary extracted from

  • Garavito, M.F.; Narvaez-Ortiz, H.Y.; Pulido, D.C.; Loeffler, M.; Judelson, H.S.; Restrepo, S.; Zimmermann, B.H.
    Phytophthora infestans dihydroorotate dehydrogenase is a potential target for chemical control - a comparison with the enzyme from Solanum tuberosum (2019), Front. Microbiol., 10, 1479 .
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.3.5.2 2-(4-biphenylylcarbamoyl)-2-benzoic acid i.e. MD 129, 0.5 mM, 103% of initial activity Phytophthora infestans
1.3.5.2 alloxan 0.5 mM, 112% of initial activity Phytophthora infestans
1.3.5.2 atovaquone 0.5 mM, 127% of initial activity Phytophthora infestans
1.3.5.2 NSC 277965 0.5 mM, 130% of initial activity Solanum tuberosum
1.3.5.2 NSC 71097 0.5 mM, 129% of initial activity Phytophthora infestans

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.5.2 expression in Escherichia coli Phytophthora infestans
1.3.5.2 expression in Escherichia coli Solanum tuberosum

Protein Variants

EC Number Protein Variants Comment Organism
1.3.5.2 additional information expression of N-terminally truncated versions lacking the transmembrane anchor Phytophthora infestans
1.3.5.2 additional information expression of N-terminally truncated versions lacking the transmembrane anchor Solanum tuberosum

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.5.2 2-(4-biphenylylcarbamoyl)-1-cyclopentene-1-carboxylic acid i.e. MD 108, 0.5 mM, 23% of initial activity Phytophthora infestans
1.3.5.2 2-(4-biphenylylcarbamoyl)-1-cyclopentene-1-carboxylic acid i.e. MD 108, 0.5 mM, 90% of initial activity Solanum tuberosum
1.3.5.2 2-(4-biphenylylcarbamoyl)-2-benzoic acid i.e. MD 129, 0.5 mM, 95% of initial activity Solanum tuberosum
1.3.5.2 A77-1726 0.5 mM, 14% of initial activity, inhibits the growth of Phytophthora infestans Phytophthora infestans
1.3.5.2 A77-1726 0.5 mM, 97% of initial activity Solanum tuberosum
1.3.5.2 alloxan 0.5 mM, 74% of initial activity Solanum tuberosum
1.3.5.2 ametoctradin 0.5 mM, 41% of initial activity Phytophthora infestans
1.3.5.2 ametoctradin 0.5 mM,102% of initial activity Solanum tuberosum
1.3.5.2 atovaquone 0.5 mM, 127% of initial activity Phytophthora infestans
1.3.5.2 atovaquone 0.5 mM, 75% of initial activity Solanum tuberosum
1.3.5.2 brequinar 0.5 mM, 98% of initial activity Phytophthora infestans
1.3.5.2 brequinar 0.5 mM, 49% of initial activity Solanum tuberosum
1.3.5.2 dichloroallyl lawsone 0.5 mM, 30% of initial activity Phytophthora infestans
1.3.5.2 dichloroallyl lawsone 0.5 mM, 39% of initial activity Solanum tuberosum
1.3.5.2 N-(3-chloro-2-methoxybiphenyl-4-yl)-2-cyano-3-hydroxybut-2-enamide i.e. MD 209, 0.5 mM, 2% of initial activity Phytophthora infestans
1.3.5.2 N-(3-chloro-2-methoxybiphenyl-4-yl)-2-cyano-3-hydroxybut-2-enamide i.e. MD 209, 0.5 mM, 0% of initial activity Solanum tuberosum
1.3.5.2 N-(3-chlorobiphenyl-4-yl)-2-cyano-3-hydroxybut-2-enamide i.e. MD 241, 0.5 mM, 1% of initial activity Phytophthora infestans
1.3.5.2 N-(3-chlorobiphenyl-4-yl)-2-cyano-3-hydroxybut-2-enamide i.e. MD 241, 0.5 mM, 87% of initial activity Solanum tuberosum
1.3.5.2 N-[3,5-difluoro-4-(trifluoromethyl)phenyl]-5-methyl[1,2,4]triazolo[1,5-a]pyrimidin-7-amine i.e. DSM190, 0.5 mM, 36% of initial activity Phytophthora infestans
1.3.5.2 N-[3,5-difluoro-4-(trifluoromethyl)phenyl]-5-methyl[1,2,4]triazolo[1,5-a]pyrimidin-7-amine i.e. DSM190, 0.5 mM, 38% of initial activity Solanum tuberosum
1.3.5.2 NSC 277965 0.5 mM, 60% of initial activity Phytophthora infestans
1.3.5.2 NSC 61890 0.5 mM, 93% of initial activity Phytophthora infestans
1.3.5.2 NSC 61890 0.5 mM, 36% of initial activity Solanum tuberosum
1.3.5.2 NSC 71097 0.5 mM, 70% of initial activity Solanum tuberosum
1.3.5.2 redoxal 0.5 mM, 31% of initial activity Phytophthora infestans
1.3.5.2 redoxal 0.5 mM, 39% of initial activity Solanum tuberosum
1.3.5.2 toltrazuril 1 mM, 67% of initial activity Phytophthora infestans
1.3.5.2 toltrazuril 1 mM, 0% of initial activity Solanum tuberosum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.5.2 0.015
-
 coenzyme QD pH 8, 30°C Phytophthora infestans
1.3.5.2 0.02
-
 (S)-dihydroorotate pH 8, 30°C Solanum tuberosum
1.3.5.2 0.03
-
 coenzyme QD pH 8, 30°C Solanum tuberosum
1.3.5.2 0.057
-
 (S)-dihydroorotate pH 8, 30°C Phytophthora infestans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.3.5.2 mitochondrion
-
 Phytophthora infestans 5739
-
1.3.5.2 mitochondrion
-
 Solanum tuberosum 5739
-

Organism

EC Number Organism UniProt Comment Textmining
1.3.5.2 Phytophthora infestans I7EMP0
-
-
1.3.5.2 Solanum tuberosum M1BCR0
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.5.2 (S)-dihydroorotate + 1,4-naphthoquinone 50% of the activity with coenzyme Qd Phytophthora infestans orotate + 1,4-naphthoquinol
-
 ?
1.3.5.2 (S)-dihydroorotate + 1,4-naphthoquinone 78% of the activity with coenzyme Qd Solanum tuberosum orotate + 1,4-naphthoquinol
-
 ?
1.3.5.2 (S)-dihydroorotate + 2,5-dimethyl-p-benzoquinone 48% of the activity with coenzyme Qd Phytophthora infestans orotate + 2,5-dimethyl-p-benzoquinol
-
 ?
1.3.5.2 (S)-dihydroorotate + 2,5-dimethyl-p-benzoquinone 67% of the activity with coenzyme Qd Solanum tuberosum orotate + 2,5-dimethyl-p-benzoquinol
-
 ?
1.3.5.2 (S)-dihydroorotate + 2,6-dihydrophenolindophenol
-
 Phytophthora infestans orotate + reduced 2,6-dihydrophenolindophenol
-
 ?
1.3.5.2 (S)-dihydroorotate + 2,6-dihydrophenolindophenol
-
 Solanum tuberosum orotate + reduced 2,6-dihydrophenolindophenol
-
 ?
1.3.5.2 (S)-dihydroorotate + 5,8-dihydroxy-1,4-naphthoquinone 78% of the activity with coenzyme Qd Solanum tuberosum orotate + 5,8-dihydroxy-1,4-naphthoquinol
-
 ?
1.3.5.2 (S)-dihydroorotate + coenzyme Q0 56% of the activity with coenzyme Qd Phytophthora infestans orotate + reduced coenzyme Q0
-
 ?
1.3.5.2 (S)-dihydroorotate + coenzyme Q0 96% of the activity with coenzyme Qd Solanum tuberosum orotate + reduced coenzyme Q0
-
 ?
1.3.5.2 (S)-dihydroorotate + coenzyme Q1 111% of the activity with coenzyme Qd Phytophthora infestans orotate + reduced coenzyme Q1
-
 ?
1.3.5.2 (S)-dihydroorotate + coenzyme Q1 124% of the activity with coenzyme Qd Solanum tuberosum orotate + reduced coenzyme Q1
-
 ?
1.3.5.2 (S)-dihydroorotate + coenzyme Q10 19% of the activity with coenzyme Qd Solanum tuberosum orotate + reduced coenzyme Q10
-
 ?
1.3.5.2 (S)-dihydroorotate + coenzyme Q10 30% of the activity with coenzyme Qd Phytophthora infestans orotate + reduced coenzyme Q10
-
 ?
1.3.5.2 (S)-dihydroorotate + coenzyme Q2 123% of the activity with coenzyme Qd Solanum tuberosum orotate + reduced coenzyme Q2
-
 ?
1.3.5.2 (S)-dihydroorotate + coenzyme Q6 102% of the activity with coenzyme Qd Solanum tuberosum orotate + reduced coenzyme Q6
-
 ?
1.3.5.2 (S)-dihydroorotate + coenzyme Q6 144% of the activity with coenzyme Qd Phytophthora infestans orotate + reduced coenzyme Q6
-
 ?
1.3.5.2 (S)-dihydroorotate + coenzyme Q9 111% of the activity with coenzyme Qd Solanum tuberosum orotate + reduced coenzyme Q9
-
 ?
1.3.5.2 (S)-dihydroorotate + coenzyme Q9 66% of the activity with coenzyme Qd Phytophthora infestans orotate + reduced coenzyme Q9
-
 ?
1.3.5.2 (S)-dihydroorotate + coenzyme Qd
-
 Phytophthora infestans orotate + reduced coenzyme Qd
-
 ?
1.3.5.2 (S)-dihydroorotate + coenzyme Qd
-
 Solanum tuberosum orotate + reduced coenzyme Qd
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.3.5.2 30
-
 (S)-dihydroorotate pH 8, 30°C Solanum tuberosum
1.3.5.2 75
-
 (S)-dihydroorotate pH 8, 30°C Phytophthora infestans

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.3.5.2 1000
-
 coenzyme QD pH 8, 30°C Solanum tuberosum
1.3.5.2 1300
-
 (S)-dihydroorotate pH 8, 30°C Phytophthora infestans
1.3.5.2 1500
-
 (S)-dihydroorotate pH 8, 30°C Solanum tuberosum
1.3.5.2 4900
-
 coenzyme QD pH 8, 30°C Phytophthora infestans