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Literature summary extracted from

  • Jia, Y.Y.; Xie, Y.L.; Yang, L.L.; Shi, H.L.; Lu, Y.F.; Zhang, S.P.; Tang, C.D.; Yao, L.G.; Kan, Y.C.
    Expression of novel L-leucine dehydrogenase and high-level production of L-tert-leucine catalyzed by engineered Escherichia coli (2021), Front. Bioeng. Biotechnol., 9, 655522 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.1.9 expression in Escherichia coli Planifilum fimeticola

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.1.9 0.06
-
 NADH pH 9.5, 30°C Planifilum fimeticola
1.4.1.9 0.1
-
 L-leucine pH 9.5, 30°C Planifilum fimeticola
1.4.1.9 0.11
-
 NAD+ pH 9.5, 30°C Planifilum fimeticola
1.4.1.9 3.08
-
 trimethylpyruvate pH 9.5, 30°C Planifilum fimeticola

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.9 Planifilum fimeticola
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.1.9 51
-
 pH 9.5, 30°C Planifilum fimeticola

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.9 2-oxobutanoate + NH3 + NADH + H+
-
 Planifilum fimeticola L-2-aminobutanoate + H2O + NAD+
-
 r
1.4.1.9 4-methyl-2-oxopentanoate + NH3 + NADH + H+
-
 Planifilum fimeticola L-leucine + H2O + NAD+
-
 r
1.4.1.9 amino(phenyl)acetate + H2O + NAD+
-
 Planifilum fimeticola oxo(phenyl)acetate + NH3 + NADH + H+
-
 r
1.4.1.9 L-leucine + H2O + NAD+
-
 Planifilum fimeticola 4-methyl-2-oxopentanoate + NH3 + NADH + H+
-
 r
1.4.1.9 L-tert-leucine + H2O + NAD+
-
 Planifilum fimeticola trimethylpyruvate + NH3 + NADH + H+
-
 r
1.4.1.9 L-valine + H2O + NAD+
-
 Planifilum fimeticola 3-methyl-2-oxobutanoate + NH3 + NADH + H+
-
 r
1.4.1.9 oxo(phenyl)acetate + NH3 + NADH + H+
-
 Planifilum fimeticola amino(phenyl)acetate + H2O + NAD+
-
 r
1.4.1.9 trimethylpyruvate + NH3 + NADH + H+
-
 Planifilum fimeticola L-tert-leucine + H2O + NAD+
-
 r

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.4.1.9 65
-
-
 Planifilum fimeticola

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.1.9 50
-
 1 h, no loss of activity Planifilum fimeticola

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.4.1.9 1.8
-
 NAD+ pH 9.5, 30°C Planifilum fimeticola
1.4.1.9 2.3
-
 L-leucine pH 9.5, 30°C Planifilum fimeticola
1.4.1.9 6.1
-
 NADH pH 9.5, 30°C Planifilum fimeticola
1.4.1.9 39.1
-
 trimethylpyruvate pH 9.5, 30°C Planifilum fimeticola

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.4.1.9 12.7
-
 trimethylpyruvate pH 9.5, 30°C Planifilum fimeticola
1.4.1.9 16.5
-
 NAD+ pH 9.5, 30°C Planifilum fimeticola
1.4.1.9 23.3
-
 L-leucine pH 9.5, 30°C Planifilum fimeticola
1.4.1.9 101
-
 NADH pH 9.5, 30°C Planifilum fimeticola