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Literature summary extracted from

  • Katsyv, A.; Schoelmerich, M.C.; Basen, M.; Mueller, V.
    The pyruvate ferredoxin oxidoreductase of the thermophilic acetogen, Thermoanaerobacter kivui (2021), FEBS open bio, 11, 1332-1342 .
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
1.2.7.1 synthesis homologous protein production in Thermoacetobacter kivui by cloning and expression in Thermoacetobacter kivui. The encoded protein containing a genetically engineered His-tag is purified in only two steps to apparent homogeneity, and has the same properties as the native Thermoanaerobacter kivui

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.7.1 0.019
-
 oxidized ferredoxin pH 7.5, 66°C Thermoanaerobacter kivui
1.2.7.1 0.025
-
 CoA pH 7.5, 66°C Thermoanaerobacter kivui
1.2.7.1 0.11
-
 pyruvate pH 7.5, 66°C Thermoanaerobacter kivui

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.7.1 Iron 13.6 mol of iron per mol of protein Thermoanaerobacter kivui

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.7.1 245000
-
 gel filtration Thermoanaerobacter kivui

Organism

EC Number Organism UniProt Comment Textmining
1.2.7.1 Thermoanaerobacter kivui A0A097APA2 isoform Pfor1, NifJ1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.7.1 purification from cells grown on glucose Thermoanaerobacter kivui

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.7.1 1.8
-
 acceptor methylene blue, pH 7.5, 66°C Thermoanaerobacter kivui
1.2.7.1 27.2
-
 acceptor ferredoxin, pH 7.5, 66°C Thermoanaerobacter kivui

Storage Stability

EC Number Storage Stability Organism
1.2.7.1 4°C, 172 days, 70% residual activity Thermoanaerobacter kivui

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.7.1 pyruvate + CoA + 2 oxidized ferredoxin
-
 Thermoanaerobacter kivui acetyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
-
 ?
1.2.7.1 pyruvate + CoA + oxidized methylene blue
-
 Thermoanaerobacter kivui acetyl-CoA + CO2 + reduced methylene blue + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.7.1 dimer 2 * 130000, SDS-PAGE Thermoanaerobacter kivui

Synonyms

EC Number Synonyms Comment Organism
1.2.7.1 NifJ1
-
 Thermoanaerobacter kivui
1.2.7.1 Pfor1
-
 Thermoanaerobacter kivui
1.2.7.1 TKV_c04340
-
 Thermoanaerobacter kivui

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.7.1 66
-
-
 Thermoanaerobacter kivui

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.7.1 7.5
-
-
 Thermoanaerobacter kivui

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.2.7.1 6
-
 20% residual activity Thermoanaerobacter kivui
1.2.7.1 8
-
 20% residual activity Thermoanaerobacter kivui

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.7.1 coenzyme A
-
 Thermoanaerobacter kivui
1.2.7.1 thiamine diphosphate
-
 Thermoanaerobacter kivui
1.2.7.1 [4Fe-4S]-center presence of three [4Fe-4S] clusters Thermoanaerobacter kivui