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Literature summary extracted from

  • Khan, M.S.; Gargiulo, S.; Soumillion, P.
    Promiscuous activity of 3-isopropylmalate dehydrogenase produced at physiological level affords Escherichia coli growth on D-malate (2020), FEBS Lett., 594, 2421-2430 .
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.85 0.011
-
 (2R,3S)-3-isopropylmalate pH 7.5, 37°C Escherichia coli
1.1.1.85 0.301
-
 D-malate pH 7.5, 37°C Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.85 (2R,3S)-3-isopropylmalate + NAD+ Escherichia coli
-
 4-methyl-2-oxopentanoate + CO2 + NADH + H+
-
 ir
1.1.1.85 (2R,3S)-3-isopropylmalate + NAD+ Escherichia coli BW25113
-
 4-methyl-2-oxopentanoate + CO2 + NADH + H+
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.85 Escherichia coli
-
-
-
1.1.1.85 Escherichia coli BW25113
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.85
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.85 (2R,3S)-3-isopropylmalate + NAD+
-
 Escherichia coli 4-methyl-2-oxopentanoate + CO2 + NADH + H+
-
 ir
1.1.1.85 (2R,3S)-3-isopropylmalate + NAD+
-
 Escherichia coli BW25113 4-methyl-2-oxopentanoate + CO2 + NADH + H+
-
 ir
1.1.1.85 D-malate + NAD+
-
 Escherichia coli pyruvate + NADH + CO2
-
 ?
1.1.1.85 D-malate + NAD+
-
 Escherichia coli BW25113 pyruvate + NADH + CO2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.85 IPMDH
-
 Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.85 0.41
-
 D-malate pH 7.5, 37°C Escherichia coli
1.1.1.85 12
-
 (2R,3S)-3-isopropylmalate pH 7.5, 37°C Escherichia coli

Expression

EC Number Organism Comment Expression
1.1.1.85 Escherichia coli induced upon leucine starvation up

General Information

EC Number General Information Comment Organism
1.1.1.85 evolution for a new phenotypic trait to appear, a genetic mutation is not strictly needed, whereas environmental conditions may play an arbitrary role in enabling or not promiscuous phenotypes. D-malate metabolism is made possible by leucine deprivation, which is a priori unrelated Escherichia coli
1.1.1.85 metabolism upon leucine starvation, the physiological concentration of the enzyme raises to a level that is sufficient for metabolizing D-malate thanks to substrate promiscuity. Under these conditions, the enzyme must also play its native role in leucine biosynthesis so the enzyme is phenotypically active in two core metabolic pathways at the same time Escherichia coli

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.85 1.36
-
 D-malate pH 7.5, 37°C Escherichia coli
1.1.1.85 1150
-
 (2R,3S)-3-isopropylmalate pH 7.5, 37°C Escherichia coli