EC Number | Application | Comment | Organism |
---|---|---|---|
1.1.3.2 | diagnostics | an improved amperometric L-lactate biosensor was constructed based on covalent immobilization of lactate oxidase from Pediococcus species onto carboxylated multiwalled carbon nanotubes (cMWCNT)/copper nanoparticles (CuNPs)/polyaniline (PANI) hybrid film electrodeposited on the surface of a pencil graphite electrode. The biosensor shows maximum response within 5 s at pH 8.0 in 0.05 M sodium phosphate buffer and 37°C, when operated at 20 mV/s. The biosensor has a detection limit of 0.00025 mM with a wide working range between 0.001-2.5 mM. The biosensor is employed for measurement of L-lactic acid level in plasma of apparently healthy and diseased persons. Analytical recovery of added lactic acid in plasma is 95.5%. The working enzyme electrode is used 180 times over a period of 140 days, when stored at 4°C | Pediococcus sp. |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.3.2 | (S)-lactate + O2 | Pediococcus sp. | - |
pyruvate + H2O2 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.3.2 | Pediococcus sp. | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.3.2 | (S)-lactate + O2 | - |
Pediococcus sp. | pyruvate + H2O2 | - |
? |