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Literature summary extracted from

  • Li, Y.; Pan, H.; Chang, Y.; Dong, N.; Zou, L.; Liang, P.; Tian, W.; Chang, Z.
    Identification of key sites determining the cofactor specificity and improvement of catalytic activity of a steroid 5beta-reductase from Capsella rubella (2020), Enzyme Microb. Technol., 134, 109483 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.3 expression in Escherichia coli Capsella rubella

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.3.1.3 homology modeling of structure Capsella rubella

Protein Variants

EC Number Protein Variants Comment Organism
1.3.1.3 R63K mutant is able to use NADH as cofactor Capsella rubella
1.3.1.3 R63K/R64H increases enzymatic activity by 13.8fold with NADH as cofactor Capsella rubella
1.3.1.3 R64G mutant exhibits significantly improved activity with NADH Capsella rubella
1.3.1.3 R64H mutant exhibits significantly improved activity with NADH Capsella rubella
1.3.1.3 R64S mutant exhibits significantly improved activity with NADH Capsella rubella
1.3.1.3 R64T mutant exhibits significantly improved activity with NADH Capsella rubella

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.1.3 0.358
-
 2-cyclohexen-1-one mutant R63K/R64H, cofactor NADPH, pH 6, 40°C Capsella rubella
1.3.1.3 0.443
-
 2-cyclohexen-1-one wild-type, cofactor NADPH, pH 6, 40°C Capsella rubella
1.3.1.3 0.541
-
 2-cyclohexen-1-one mutant R63K/R64H, cofactor NADH, pH 6, 40°C Capsella rubella

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.3 Capsella rubella R0F4W3
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.3 (20S)-21-hydroxy-20-methylpregn-4-en-3-one + NADPH + H+
-
 Capsella rubella ? + NADP+
-
 ?
1.3.1.3 2-cyclohexen-1-one + NADPH + H+
-
 Capsella rubella ? + NADP+
-
 ?
1.3.1.3 4-androstene-3,17-dione + NADPH + H+
-
 Capsella rubella 5beta-androstan-3,17-dione + NADP+
-
 ?
1.3.1.3 ethyl acrylate + NADPH + H+
-
 Capsella rubella ? + NADP+
-
 ?
1.3.1.3 progesterone + NADPH + H+
-
 Capsella rubella 5beta-pregnane-3,20-dione + NADP+
-
 ?
1.3.1.3 testosterone + NADPH + H+
-
 Capsella rubella (5beta,17beta)-17-hydroxyandrostan-3-one + NADP+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.3.1.3 CARUB_v10005016mg
-
 Capsella rubella
1.3.1.3 St5betaR1
-
 Capsella rubella

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.3.1.3 0.23
-
 2-cyclohexen-1-one wild-type, cofactor NADPH, pH 6, 40°C Capsella rubella
1.3.1.3 0.28
-
 2-cyclohexen-1-one mutant R63K/R64H, cofactor NADPH, pH 6, 40°C Capsella rubella
1.3.1.3 0.81
-
 2-cyclohexen-1-one mutant R63K/R64H, cofactor NADH, pH 6, 40°C Capsella rubella

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.3 NADPH wild-type is strictly dependen on NADPH Capsella rubella

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.3.1.3 0.52
-
 2-cyclohexen-1-one wild-type, cofactor NADPH, pH 6, 40°C Capsella rubella
1.3.1.3 0.611
-
 2-cyclohexen-1-one mutant R63K/R64H, cofactor NADPH, pH 6, 40°C Capsella rubella
1.3.1.3 1.5
-
 2-cyclohexen-1-one mutant R63K/R64H, cofactor NADH, pH 6, 40°C Capsella rubella