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Literature summary extracted from

  • Kalimuthu, P.; Daumann, L.J.; Pol, A.; Op den Camp, H.J.M.; Bernhardt, P.V.
    Electrocatalysis of a europium-dependent bacterial methanol dehydrogenase with its physiological electron-acceptor cytochrome cGJ (2019), Chemistry, 25, 8760-8768 .
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.2.10 additional information
-
 additional information Michaelis-Menten kinetics Methylacidiphilum fumariolicum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.2.10 Eu3+ the enzyme is dependent on the lanthanide europium Methylacidiphilum fumariolicum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.2.10 methanol + 2 cytochrome cGJ Methylacidiphilum fumariolicum
-
 formaldehyde + 2 reduced cytochrome cGJ
-
 r
1.1.2.10 methanol + 2 cytochrome cGJ Methylacidiphilum fumariolicum SolV
-
 formaldehyde + 2 reduced cytochrome cGJ
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.2.10 Methylacidiphilum fumariolicum
-
-
-
1.1.2.10 Methylacidiphilum fumariolicum SolV
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.2.10 methanol + 2 cytochrome cGJ
-
 Methylacidiphilum fumariolicum formaldehyde + 2 reduced cytochrome cGJ
-
 r
1.1.2.10 methanol + 2 cytochrome cGJ electrochemic reaction, use of a Au/MU electrode for the electrontransfer process. The attenuation coefficient of DCPIP is known to be very sensitive to changes in pH, reactions with DCPIP are monitored at 600 nm Methylacidiphilum fumariolicum formaldehyde + 2 reduced cytochrome cGJ
-
 r
1.1.2.10 methanol + 2 cytochrome cGJ
-
 Methylacidiphilum fumariolicum SolV formaldehyde + 2 reduced cytochrome cGJ
-
 r
1.1.2.10 methanol + 2 cytochrome cGJ electrochemic reaction, use of a Au/MU electrode for the electrontransfer process. The attenuation coefficient of DCPIP is known to be very sensitive to changes in pH, reactions with DCPIP are monitored at 600 nm Methylacidiphilum fumariolicum SolV formaldehyde + 2 reduced cytochrome cGJ
-
 r

Synonyms

EC Number Synonyms Comment Organism
1.1.2.10 Eu-MDH
-
 Methylacidiphilum fumariolicum
1.1.2.10 lanthanoid-dependent methanol dehydrogenase
-
 Methylacidiphilum fumariolicum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.2.10 45
-
 assay at Methylacidiphilum fumariolicum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.2.10 7.3
-
-
 Methylacidiphilum fumariolicum

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.2.10 2,7,9-tricarboxypyrroloquinoline quinone Eu-MDH harbours a redox active 2,7,9-tricarboxypyrroloquinoline quinone (PQQ) cofactor which is non-covalently bound but coordinates trivalent lanthanoid elements including Eu3+ Methylacidiphilum fumariolicum
1.1.2.10 cytochrome cGJ
-
 Methylacidiphilum fumariolicum

General Information

EC Number General Information Comment Organism
1.1.2.10 physiological function lanthanoid-dependent methanol dehydrogenase (Eu-MDH) from the acidophilic verrucomicrobial methanotroph Methylacidiphilum fumariolicum SolV has its own physiological cytochrome cGJ electron acceptor. Eu-MDH harbours a redox active 2,7,9-tricarboxypyrroloquinoline quinone (PQQ) cofactor which is non-covalently bound but coordinates trivalent lanthanoid elements including Eu3+. Eu-MDH and the cytochrome are co-adsorbed with the biopolymer chitosan and cast onto a mercaptoundecanol (MU) monolayer modified Au working electrode. Cyclic voltammetry of cytochrome cGJ reveals a well-defined quasi-reversible FeIII/II redox couple at +255 mV versus normal hydrogen electrode (NHE) at pH 7.5, and this response is pH independent. The reversible one-electron response of the cytochrome cGJ transforms into a sigmoidal catalytic wave in the presence of Eu-MDH and its substrates (methanol or formaldehyde). The catalytic current is pH-dependent, and pH 7.3 is optimal Methylacidiphilum fumariolicum