Any feedback?
Literature summary extracted from
- Expansion of the substrate specificity of porcine kidney D-amino acid oxidase for S-stereoselective oxidation of 4-Cl-benzhydrylamine (2018), ChemCatChem, 10, 3500-3505 .
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 1.4.3.3 | I230A/R283G | mutant accepts 1-(4-chlorophenyl)-1-phenylmethanamine as substrate, activity with (R)-1-phenylethylamine is diminished 10fold as compared with the Y228L/R283G variant | Sus scrofa |
| 1.4.3.3 | I230C/R283G | accepts 1-(4-chlorophenyl)-1-phenylmethanamine as substrate | Sus scrofa |
| 1.4.3.3 | I230F/R283G | accepts 1-(4-chlorophenyl)-1-phenylmethanamine as substrate | Sus scrofa |
| 1.4.3.3 | R283G | mutant has completely lost the activity for D-amino acids but accepts 1-(4-chlorophenyl)-1-phenylmethanamine as substrate | Sus scrofa |
| 1.4.3.3 | Y228L | about 20% of wild-type activity wih phenylalanine | Sus scrofa |
| 1.4.3.3 | Y228L/R283G | mutant accepts 1-(4-chlorophenyl)-1-phenylmethanamine as substrate | Sus scrofa |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.4.3.3 | 2.4 | - |
1,1-diphenylmethanamine | mutant I230C/R283G, 30°C, pH 8.0 | Sus scrofa | |
| 1.4.3.3 | 2.63 | - |
1,1-diphenylmethanamine | mutant I230F/R283G, 30°C, pH 8.0 | Sus scrofa | |
| 1.4.3.3 | 2.94 | - |
(RS)-1-(4-chlorophenyl)-1-phenylmethanamine | mutant I230A/R283G, 30°C, pH 8.0 | Sus scrofa | |
| 1.4.3.3 | 2.96 | - |
(RS)-1-(4-chlorophenyl)-1-phenylmethanamine | mutant I230C/R283G, 30°C, pH 8.0 | Sus scrofa | |
| 1.4.3.3 | 3.73 | - |
(RS)-1-(4-chlorophenyl)-1-phenylmethanamine | mutant I230F/R283G, 30°C, pH 8.0 | Sus scrofa | |
| 1.4.3.3 | 5.01 | - |
D-phenylalanine | wild-type, 30°C, pH 8.0 | Sus scrofa |  |
| 1.4.3.3 | 5.23 | - |
1,1-diphenylmethanamine | mutant I230A/R283G, 30°C, pH 8.0 | Sus scrofa | |
| 1.4.3.3 | 6.25 | - |
(1R)-1-phenylethan-1-amine | mutant I230F/R283G, 30°C, pH 8.0 | Sus scrofa | |
| 1.4.3.3 | 6.9 | - |
(1R)-1-phenylethan-1-amine | mutant I230C/R283G, 30°C, pH 8.0 | Sus scrofa | |
| 1.4.3.3 | 6.98 | - |
(1R)-1-phenylethan-1-amine | mutant R283G, 30°C, pH 8.0 | Sus scrofa | |
| 1.4.3.3 | 7.28 | - |
(1R)-1-phenylethan-1-amine | mutant I230A/R283G, 30°C, pH 8.0 | Sus scrofa | |
| 1.4.3.3 | 7.95 | - |
(1R)-1-phenylethan-1-amine | mutant Y228L/R283G, 30°C, pH 8.0 | Sus scrofa | |
| 1.4.3.3 | 8.41 | - |
D-phenylalanine | mutant Y228L, 30°C, pH 8.0 | Sus scrofa | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.4.3.3 | Sus scrofa | P00371 | - |
- |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.4.3.3 | (1R)-1-phenylethan-1-amine + H2O + O2 | no substrate of wild-type | Sus scrofa | 1-phenylethan-1-one + NH3 + H2O2 | - |
? | |
| 1.4.3.3 | (RS)-1-(4-chlorophenyl)-1-phenylmethanamine + H2O + O2 | no substrate of wild-type | Sus scrofa | (4-chlorophenyl)(phenyl)methanone + NH3 + H2O2 | - |
? | |
| 1.4.3.3 | 1,1-diphenylmethanamine + H2O + O2 | no substrate of wild-type | Sus scrofa | diphenylmethanone + NH3 + H2O2 | - |
? | |
| 1.4.3.3 | D-phenylalanine + H2O + O2 | - |
Sus scrofa | phenylpyruvic acid + NH3 + H2O2 | - |
? | |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.4.3.3 | 0.047 | - |
(1R)-1-phenylethan-1-amine | mutant I230F/R283G, 30°C, pH 8.0 | Sus scrofa | |
| 1.4.3.3 | 0.111 | - |
(1R)-1-phenylethan-1-amine | mutant I230C/R283G, 30°C, pH 8.0 | Sus scrofa | |
| 1.4.3.3 | 0.349 | - |
(1R)-1-phenylethan-1-amine | mutant I230A/R283G, 30°C, pH 8.0 | Sus scrofa | |
| 1.4.3.3 | 0.366 | - |
1,1-diphenylmethanamine | mutant I230F/R283G, 30°C, pH 8.0 | Sus scrofa | |
| 1.4.3.3 | 1.46 | - |
(1R)-1-phenylethan-1-amine | mutant R283G, 30°C, pH 8.0 | Sus scrofa | |
| 1.4.3.3 | 1.72 | - |
D-phenylalanine | mutant Y228L, 30°C, pH 8.0 | Sus scrofa | |
| 1.4.3.3 | 3.3 | - |
1,1-diphenylmethanamine | mutant I230A/R283G, 30°C, pH 8.0 | Sus scrofa | |
| 1.4.3.3 | 3.42 | - |
1,1-diphenylmethanamine | mutant I230C/R283G, 30°C, pH 8.0 | Sus scrofa | |
| 1.4.3.3 | 4.33 | - |
(RS)-1-(4-chlorophenyl)-1-phenylmethanamine | mutant I230F/R283G, 30°C, pH 8.0 | Sus scrofa | |
| 1.4.3.3 | 5.19 | - |
D-phenylalanine | wild-type, 30°C, pH 8.0 | Sus scrofa | |
| 1.4.3.3 | 5.48 | - |
(RS)-1-(4-chlorophenyl)-1-phenylmethanamine | mutant I230C/R283G, 30°C, pH 8.0 | Sus scrofa | |
| 1.4.3.3 | 6.01 | - |
(RS)-1-(4-chlorophenyl)-1-phenylmethanamine | mutant I230A/R283G, 30°C, pH 8.0 | Sus scrofa | |
| 1.4.3.3 | 9.93 | - |
(1R)-1-phenylethan-1-amine | mutant Y228L/R283G, 30°C, pH 8.0 | Sus scrofa | |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.4.3.3 | 0.008 | - |
(1R)-1-phenylethan-1-amine | mutant I230F/R283G, 30°C, pH 8.0 | Sus scrofa | |
| 1.4.3.3 | 0.016 | - |
(1R)-1-phenylethan-1-amine | mutant I230C/R283G, 30°C, pH 8.0 | Sus scrofa | |
| 1.4.3.3 | 0.048 | - |
(1R)-1-phenylethan-1-amine | mutant I230A/R283G, 30°C, pH 8.0 | Sus scrofa | |
| 1.4.3.3 | 0.141 | - |
1,1-diphenylmethanamine | mutant I230F/R283G, 30°C, pH 8.0 | Sus scrofa | |
| 1.4.3.3 | 0.21 | - |
(1R)-1-phenylethan-1-amine | mutant R283G, 30°C, pH 8.0 | Sus scrofa | |
| 1.4.3.3 | 0.215 | - |
D-phenylalanine | mutant Y228L, 30°C, pH 8.0 | Sus scrofa | |
| 1.4.3.3 | 0.629 | - |
1,1-diphenylmethanamine | mutant I230A/R283G, 30°C, pH 8.0 | Sus scrofa | |
| 1.4.3.3 | 1.04 | - |
D-phenylalanine | wild-type, 30°C, pH 8.0 | Sus scrofa | |
| 1.4.3.3 | 1.15 | - |
(RS)-1-(4-chlorophenyl)-1-phenylmethanamine | mutant I230F/R283G, 30°C, pH 8.0 | Sus scrofa | |
| 1.4.3.3 | 1.26 | - |
(1R)-1-phenylethan-1-amine | mutant Y228L/R283G, 30°C, pH 8.0 | Sus scrofa | |
| 1.4.3.3 | 1.45 | - |
1,1-diphenylmethanamine | mutant I230C/R283G, 30°C, pH 8.0 | Sus scrofa | |
| 1.4.3.3 | 1.85 | - |
(RS)-1-(4-chlorophenyl)-1-phenylmethanamine | mutant I230C/R283G, 30°C, pH 8.0 | Sus scrofa | |
| 1.4.3.3 | 2.05 | - |
(RS)-1-(4-chlorophenyl)-1-phenylmethanamine | mutant I230A/R283G, 30°C, pH 8.0 | Sus scrofa |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
