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Literature summary extracted from

  • Nguyen, Q.-T.; de Gonzalo, G.; Binda, C.; Rioz-Martinez, A.; Mattevi, A.; Fraaije, M.W.
    Biocatalytic properties and structural analysis of eugenol oxidase from Rhodococcus jostii RHA1 A versatile oxidative biocatalyst (2016), Chembiochem, 17, 1359-1366 .
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.3.B5 structures in complexes with isoeugenol, coniferyl alcohol, vanillin, and benzoate. The catalytic center is a remarkable solvent-inaccessible cavity on the si side of the flavin cofactor. Structural comparison with vanillyl alcohol oxidase from Penicillium simplicissimum Rhodococcus jostii

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
1.1.3.B5 diisopropyl ether increases selectivtiy and conversion rate of the reaction with 4-(1-hydroxyethyl)-2-methoxyphenol Rhodococcus jostii
1.1.3.B5 DMSO 10% (v/v), increase in the enzymatic activity Rhodococcus jostii
1.1.3.B5 Ethyl acetate increase in enzymatic activity Rhodococcus jostii

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.B5 Rhodococcus jostii Q0SBK1
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.B5 4-(1-hydroxyethyl)-2-methoxyphenol + O2 racemic analogue of vanillyl alcohol Rhodococcus jostii 1-(4-hydroxy-3-methoxyphenyl)ethan-1-one + 4-[(1S)-1-hydroxyethyl]-2-methoxyphenol + ?
-
 ?
1.1.3.B5 4-(4-hydroxyphenyl)butan-2-one + O2
-
 Rhodococcus jostii (3E)-4-(4-hydroxyphenyl)but-3-en-2-one + ?
-
 ?
1.1.3.B5 eugenol + O2 + H2O
-
 Rhodococcus jostii coniferyl alcohol + H2O2
-
 ?
1.1.3.B5 indan-5-ol + O2
-
 Rhodococcus jostii 1H-inden-6-ol + 5-hydroxyindan-1-one + ?
-
 ?
1.1.3.B5 additional information EUGO can efficiently catalyze the dehydrogenation of various phenolic ketones and the selective oxidation of a racemic 4-(1-hydroxyethyl)-2-methoxyphenol. Very poor substrates: 4-(1,3-dithiolan-2-yl)phenol, capsaicin, (4-hydroxyphenyl)acetonitrile, 3-(4-hydroxyphenyl)propanoic acid, 2-methoxy-4-methylphenol, and 4-(hept-1-enyl)-2-methoxyphenol Rhodococcus jostii ?
-
-
1.1.3.B5 vanillyl alcohol + O2
-
 Rhodococcus jostii vanillin + H2O2
-
 ?
1.1.3.B5 zingerone + O2
-
 Rhodococcus jostii dehydrozingerone + H2O2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.3.B5 EUGO
-
 Rhodococcus jostii
1.1.3.B5 RHA1_ro03282
-
 Rhodococcus jostii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.3.B5 65
-
 melting temperature, pH 5-8. The presence of 10% (v/v) DMSO or ethyl acetate does not significantly affect the enzyme's thermostability Rhodococcus jostii