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Literature summary extracted from

  • Waldman, A.; Pechersky, Y.; Wang, P.; Wang, J.; Balskus, E.
    The cremeomycin biosynthetic gene cluster encodes a pathway for diazo formation (2015), ChemBioChem, 16, 2172-2175 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.13.249 expression in Streptomyces lividans Streptomyces cremeus
2.1.1.380 expression in Escherichia coli Streptomyces cremeus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.13.249 3-amino-4-hydroxybenzoate + NADPH + H+ + O2 Streptomyces cremeus
-
 3-amino-2,4-dihydroxybenzoate + NADP+ + H2O
-
 ?
2.1.1.380 S-adenosyl-L-methionine + 3-amino-2,4-dihydroxybenzoate Streptomyces cremeus
-
 S-adenosyl-L-homocysteine + 3-amino-2-hydroxy-4-methoxybenzoate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.249 Streptomyces cremeus A0A0K2JKU1
-
-
2.1.1.380 Streptomyces cremeus A0A0K2JL91
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.249 3-amino-4-hydroxybenzoate + NADPH + H+ + O2
-
 Streptomyces cremeus 3-amino-2,4-dihydroxybenzoate + NADP+ + H2O
-
 ?
1.14.13.249 additional information no substrate: 3-amino-4-methoxybenzoic acid Streptomyces cremeus ?
-
-
2.1.1.380 S-adenosyl-L-methionine + 3-amino-2,4-dihydroxybenzoate
-
 Streptomyces cremeus S-adenosyl-L-homocysteine + 3-amino-2-hydroxy-4-methoxybenzoate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.13.249 creL
-
 Streptomyces cremeus
2.1.1.380 creN
-
 Streptomyces cremeus

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.249 FAD
-
 Streptomyces cremeus

General Information

EC Number General Information Comment Organism
1.14.13.249 physiological function enzyme is part of the gene cluster that produces the o-diazoquinone natural product cremeomycin, involved in 3-amino-4-hydroxybenzoic acid synthesis and tailoring. During biosynthesis, hydroxylation precedes O-methylation in the transformation of 3-amino-4-hydroxybenzoate to 3-amino-2-hydroxy-4-methoxybenzoic acid Streptomyces cremeus
2.1.1.380 physiological function enzyme is involved in cremeomycin biosynthesis Streptomyces cremeus