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Literature summary extracted from

  • Cheng, X.; Chen, X.; Feng, J.; Wu, Q.; Zhu, D.
    Structure-guided engineering of meso-diaminopimelate dehydrogenase for enantioselective reductive amination of sterically bulky 2-keto acids (2018), Catal. Sci. Technol., 8, 4994-5002 .
No PubMed abstract available

Protein Variants

EC Number Protein Variants Comment Organism
1.4.1.16 H227I mutant accepts substrates D-2-phenylglycine and D-homophenylalanine Symbiobacterium thermophilum
1.4.1.16 H227V mutant shows high activity toward phenylpyruvic acid and 2-oxo-4-phenylbutyric acid Symbiobacterium thermophilum
1.4.1.16 T171H mutant accepts substrates D-2-phenylglycine and D-homophenylalanine Symbiobacterium thermophilum
1.4.1.16 W121L mutant accepts substrates D-2-phenylglycine and D-homophenylalanine Symbiobacterium thermophilum
1.4.1.16 W121L/H227I mutant sow improved enzyme activities towards various 2-oxoacids including sterically bulky substrates. The substrate binding cavity of the mutant enzyme is reshaped to accommodate these bulky substrates, thus leading to higher enzyme activity Symbiobacterium thermophilum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.1.16 12.2
-
2-oxo-3-phenylpropanoate mutant W121L/H227I, pH 9, 30°C Symbiobacterium thermophilum
1.4.1.16 17.72
-
2-oxo-4-methylpentanoate mutant W121L/H227I, pH 9, 30°C Symbiobacterium thermophilum
1.4.1.16 23.5
-
2-oxopentanoate mutant W121L/H227I, pH 9, 30°C Symbiobacterium thermophilum
1.4.1.16 33.83
-
2-oxo-4-phenylbutanoate mutant W121L/H227I, pH 9, 30°C Symbiobacterium thermophilum
1.4.1.16 35.12
-
2-oxobutanoate mutant W121L/H227I, pH 9, 30°C Symbiobacterium thermophilum
1.4.1.16 46.1
-
2-oxo-3-methylbutanoate mutant W121L/H227I, pH 9, 30°C Symbiobacterium thermophilum
1.4.1.16 48.46
-
pyruvate mutant W121L/H227I, pH 9, 30°C Symbiobacterium thermophilum

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.16 Symbiobacterium thermophilum Q67PI3
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.16 2-oxo(phenyl)acetate + NH3 + NADPH + H+
-
Symbiobacterium thermophilum (2S)-amino(phenyl)acetate + NADP+
-
r
1.4.1.16 2-oxo-3,3-dimethylbutanoate + NH3 + NADPH + H+
-
Symbiobacterium thermophilum (2S)-2-amino-3,3-dimethylbutanoate + NADP+
-
r
1.4.1.16 2-oxo-3-methylbutanoate + NH3 + NADPH + H+
-
Symbiobacterium thermophilum (2S)-2-amino-3-methylbutanoate + NADP+
-
r
1.4.1.16 2-oxo-3-phenylpropanoate + NH3 + NADPH + H+
-
Symbiobacterium thermophilum (2S)-2-amino-3-phenylpropanoate + NADP+
-
r
1.4.1.16 2-oxo-4-methylpentanoate + NH3 + NADPH + H+
-
Symbiobacterium thermophilum (2S)-2-amino-4-methylpentanoate + NADP+
-
r
1.4.1.16 2-oxo-4-phenylbutanoate + NH3 + NADPH + H+
-
Symbiobacterium thermophilum (2S)-2-amino-4-phenylbutanoate + NADP+
-
r
1.4.1.16 2-oxobutanoate + NH3 + NADPH + H+
-
Symbiobacterium thermophilum (2S)-2-aminobutanoate + NADP+
-
r
1.4.1.16 2-oxopentanoate + NH3 + NADPH + H+
-
Symbiobacterium thermophilum (2S)-2-aminopentanoate + NADP+
-
r
1.4.1.16 3-(1H-indol-3-yl)-2-oxopropanoate + NH3 + NADPH + H+
-
Symbiobacterium thermophilum (2S)-2-amino-3-(1H-indol-3-yl)propanoate + NADP+
-
r
1.4.1.16 pyruvate + NH3 + NADPH + H+
-
Symbiobacterium thermophilum D-alanine + NADP+
-
r

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.4.1.16 5.17
-
2-oxo-3-methylbutanoate mutant W121L/H227I, pH 9, 30°C Symbiobacterium thermophilum
1.4.1.16 6.2
-
2-oxo-3-phenylpropanoate mutant W121L/H227I, pH 9, 30°C Symbiobacterium thermophilum
1.4.1.16 12.6
-
2-oxo-4-phenylbutanoate mutant W121L/H227I, pH 9, 30°C Symbiobacterium thermophilum
1.4.1.16 13.49
-
2-oxo-4-methylpentanoate mutant W121L/H227I, pH 9, 30°C Symbiobacterium thermophilum
1.4.1.16 35.38
-
2-oxobutanoate mutant W121L/H227I, pH 9, 30°C Symbiobacterium thermophilum
1.4.1.16 38.7
-
2-oxopentanoate mutant W121L/H227I, pH 9, 30°C Symbiobacterium thermophilum
1.4.1.16 45.22
-
pyruvate mutant W121L/H227I, pH 9, 30°C Symbiobacterium thermophilum