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Literature summary extracted from

  • Nishimura, M.; Kawakami, S.; Otsuka, H.
    Molecular cloning and characterization of vanillin dehydrogenase from Streptomyces sp. NL15-2K (2018), BMC Microbiol., 18, 154 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.67 expressed in Escherichia coli BL21(DE3) cells Streptomyces sp. NL15-2K

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.67 Ag+ complete inhibition at 1 mM Streptomyces sp. NL15-2K
1.2.1.67 Cu2+ 79% inhibition at 1 mM Streptomyces sp. NL15-2K
1.2.1.67 additional information not affected by Co2+, Fe2+, Mg2+, Mn2+, and Fe3+ Streptomyces sp. NL15-2K
1.2.1.67 Zn2+ 15% inhibition at 1 mM Streptomyces sp. NL15-2K

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.67 0.00847
-
 Vanillin at pH 9.5 and 45°C Streptomyces sp. NL15-2K
1.2.1.67 0.566
-
 Salicylaldehyde at pH 9.5 and 45°C Streptomyces sp. NL15-2K
1.2.1.67 0.647
-
 NAD+ at pH 9.5 and 45°C Streptomyces sp. NL15-2K

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.67 214000
-
 gel filtration Streptomyces sp. NL15-2K

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.67 Streptomyces sp. NL15-2K
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.67 DEAE-Sepharose column chromatography, Resource Phe column chromatography, and Mono Q column chromatography Streptomyces sp. NL15-2K

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.67 4-hydroxybenzaldehyde + NAD+ + H2O 56% activity compared to vanillin Streptomyces sp. NL15-2K 4-hydroxybenzoate + NADH + 2 H+
-
 ?
1.2.1.67 anisaldehyde + NAD+ + H2O 15% activity compared to vanillin Streptomyces sp. NL15-2K anisoate + NADH + 2 H+
-
 ?
1.2.1.67 benzaldehyde + NAD+ + H2O 79% activity compared to vanillin Streptomyces sp. NL15-2K benzoate + NADH + 2 H+
-
 ?
1.2.1.67 isovanillin + NAD+ + H2O 49% activity compared to vanillin Streptomyces sp. NL15-2K isovanillate + NADH + 2 H+
-
 ?
1.2.1.67 additional information no activity with NADP+ or syringaldehyde Streptomyces sp. NL15-2K ?
-
-
1.2.1.67 protocatechualdehyde + NAD+ + H2O 91% activity compared to vanillin Streptomyces sp. NL15-2K protocatechuate + NADH + 2 H+
-
 ?
1.2.1.67 salicylaldehyde + NAD+ + H2O 48% activity compared to vanillin Streptomyces sp. NL15-2K salicylate + NADH + 2 H+
-
 ?
1.2.1.67 vanillin + NAD+ + H2O 100% activity Streptomyces sp. NL15-2K vanillate + NADH + 2 H+
-
 ?
1.2.1.67 veratraldehyde + NAD+ + H2O 12% activity compared to vanillin Streptomyces sp. NL15-2K veratrate + NADH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.1.67 homotetramer 4 * 56000, SDS-PAGE Streptomyces sp. NL15-2K
1.2.1.67 homotetramer 4 * 51705, calculated from amino acid sequence Streptomyces sp. NL15-2K

Synonyms

EC Number Synonyms Comment Organism
1.2.1.67 VDH
-
 Streptomyces sp. NL15-2K

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.1.67 45
-
-
 Streptomyces sp. NL15-2K

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.2.1.67 20 70 after 10 min at 20-70°C, the enzyme activity is retained up to 40°C but declines sharply above 45°C, and the enzyme solution incubated at 50°C becomes slightly clouded Streptomyces sp. NL15-2K

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.2.1.67 9.46
-
 Vanillin at pH 9.5 and 45°C Streptomyces sp. NL15-2K
1.2.1.67 14.91
-
 Salicylaldehyde at pH 9.5 and 45°C Streptomyces sp. NL15-2K
1.2.1.67 23.45
-
 NAD+ at pH 9.5 and 45°C Streptomyces sp. NL15-2K

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.67 9.5
-
-
 Streptomyces sp. NL15-2K

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.2.1.67 4 11 at all pH ranges tested, the enzyme loses more than 40% of its initial activity after storage at 4°C for 15 h, and the enzyme solution becomes clouded after storage at pH 5.0 or less Streptomyces sp. NL15-2K

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.67 NAD+
-
 Streptomyces sp. NL15-2K

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.2.1.67 Streptomyces sp. NL15-2K calculated from amino acid sequence
-
 5

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.2.1.67 26
-
 Salicylaldehyde at pH 9.5 and 45°C Streptomyces sp. NL15-2K
1.2.1.67 36
-
 NAD+ at pH 9.5 and 45°C Streptomyces sp. NL15-2K
1.2.1.67 1110
-
 Vanillin at pH 9.5 and 45°C Streptomyces sp. NL15-2K