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Literature summary extracted from

  • Cui, Q.; Zhou, F.; Liu, W.; Tao, Y.
    Avermectin biosynthesis stable functional expression of branched chain alpha-keto acid dehydrogenase complex from Streptomyces avermitilis in Escherichia coli by selectively regulating individual subunit gene expression (2017), Biotechnol. Lett., 39, 1567-1574 .
    View publication on PubMed

Application

EC Number Application Comment Organism
1.2.4.4 synthesis functional expression of the branched chain alpha-keto acid dehydrogenase complex in Escherichia coli by independent and selective optimization of individual subunit genes of the complex. Codon optimization significantly improves the expression of complex component proteins BkdH and LpdA1 but expression of dehydrogenase E1 alpha subunit BkdF and dehydrogenase E1 beta subunit BkdG depends on coexpression of the BkdH gene. The optimized branched chain alpha-keto acid dehydrogenase complex supplies sufficient short branched-chain acyl-CoA to synthesize phlorisovalerophenone Streptomyces avermitilis
2.3.1.168 synthesis functional expression of the branched chain alpha-keto acid dehydrogenase complex in Escherichia coli by independent and selective optimization of individual subunit genes of the complex. Codon optimization significantly improves the expression of complex component proteins BkdH and LpdA1 but expression of dehydrogenase E1 alpha subunit BkdF and dehydrogenase E1 beta subunit BkdG depends on coexpression of the BkdH gene. The optimized branched chain alpha-keto acid dehydrogenase complex supplies sufficient short branched-chain acyl-CoA to synthesize phlorisovalerophenone Streptomyces avermitilis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.25 gene bkdA, the BKDH complex from Streptomyces avermitilis cannot readily be stably expressed in Escherichia coli. This is mostly due to low yields of active, properly folded enzyme in a complex expression system. Codon optimization, recombinant expression of the enzyme complex in Escherichia coli strain BW25113 by selectively regulating individual subunit gene expression Streptomyces avermitilis
1.2.4.4 expression in Escherichia coli Streptomyces avermitilis
2.3.1.168 expression in Escherichia coli Streptomyces avermitilis

Protein Variants

EC Number Protein Variants Comment Organism
1.2.1.25 additional information improvement of the production of short branched-chain acyl-CoAs for avermectin biosynthesis by functional expression of the branched chain alpha-keto acid dehydrogenase complex (BKDH) from Streptomyces avermitilis, systematic optimization by selectively regulating individual subunit expression in Escherichia coli, codon optimization, method, overview. Synergic synthesis pathway of avermectin precursors. Codon optimization could improve the expression of BkdH and LpdA1 from pRB1a, we further coexpressed the bkdF and bkdG with wild-type or codon optimized bkdH and lpdA1 by constructing vectors pRB01 (wild-type) and pRB02 (optimized). The bkdF-bkdG and bkdH-lpdA1 gene clusters were placed under the control of two independent araBAD promoters in pRB1a. Genes bkdF and bkdG are respectively inserted close to the araBAD promoters so as to enhance their expression Streptomyces avermitilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.25 3-methyl-2-oxobutanoate + CoA + NAD+ Streptomyces avermitilis
-
 2-methylpropanoyl-CoA + CO2 + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.25 Streptomyces avermitilis Q53592 BKDH complex component E1
-
1.2.4.4 Streptomyces avermitilis
-
-
-
2.3.1.168 Streptomyces avermitilis
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.25 3-methyl-2-oxobutanoate + CoA + NAD+
-
 Streptomyces avermitilis 2-methylpropanoyl-CoA + CO2 + NADH + H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.2.1.25 bkdA
-
 Streptomyces avermitilis
1.2.1.25 BKDH
-
 Streptomyces avermitilis
1.2.1.25 branched chain alpha-keto acid dehydrogenase complex
-
 Streptomyces avermitilis
1.2.4.4 BDKG
-
 Streptomyces avermitilis
1.2.4.4 bkdF
-
 Streptomyces avermitilis
2.3.1.168 BKDH
-
 Streptomyces avermitilis

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.25 CoA
-
 Streptomyces avermitilis
1.2.1.25 NAD+
-
 Streptomyces avermitilis
1.2.1.25 thiamine diphosphate
-
 Streptomyces avermitilis

General Information

EC Number General Information Comment Organism
1.2.1.25 physiological function the BKDH complex is a multienzyme complex composed of three catalytic components: a thiamine diphosphate-dependent decarboxylase/dehydrogenase (E1), a lipoyl transacylase (E2), and a dihydrolipoamide dehydrogenase (E3) Streptomyces avermitilis