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Literature summary extracted from

  • Zhou, Y.; Wei, Y.; Nanjaraj Urs, A.N.; Lin, L.; Xu, T.; Hu, Y.; Ang, E.L.; Zhao, H.; Yuchi, Z.; Zhang, Y.
    Identification and characterization of a new sulfoacetaldehyde reductase from the human gut bacterium Bifidobacterium kashiwanohense (2019), Biosci. Rep., 39, BSR20190715 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.313 gene isfD, genetic structure Chromohalobacter salexigens
1.1.1.433 expression in Escherichia coli Bifidobacterium catenulatum subsp. kashiwanohense
1.1.1.433 gene sarD, genetic structure Bilophila wadsworthia
1.1.1.433 gene tauF, genetic structure, sequence comparisons, recombinant expression as His6-MBP-tagged enzyme in Escherichia coli strain BL21(DE3) from HMT-GGG-BkTauF plasmid Bifidobacterium catenulatum subsp. kashiwanohense

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.433 purifed recombinant BkTauF in the apo form and in a binary complex with NAD+, hanging drop vapor diffusion method, mixing of 10 mg/ml protein in 20 mM Tris, pH 7.5, 200 mM KCl, 1 mM DTT, with reservoir solution containing 0.2 M ammonium acetate, 0.1 M Bis-Tris, pH 5.5, and 25% w/v PEG 3350, 22°C, method optimization, X-ray diffraction structure determination and analysis at 1.9 and 3.0 A resolution, respectively, molecular replacement using structure with PDB ID 1VHD as a search model, modeling Bifidobacterium catenulatum subsp. kashiwanohense
1.1.1.433 structures in the apo form and in a binary complex with NAD+ at 1.9 and 3.0 A resolution, respectively Bifidobacterium catenulatum subsp. kashiwanohense

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.433 F252A site-directed mutagenesis, the mutant shows 78% reduced activity for isethionate oxidation compared to wild-type enzyme Bifidobacterium catenulatum subsp. kashiwanohense
1.1.1.433 F252A mutant retains approximately 28% of the wild-type activity Bifidobacterium catenulatum subsp. kashiwanohense
1.1.1.433 F265A almost complete loss of activity Bifidobacterium catenulatum subsp. kashiwanohense
1.1.1.433 F265A site-directed mutagenesis, the mutant shows highly reduced activity for isethionate oxidation compared to wild-type enzyme Bifidobacterium catenulatum subsp. kashiwanohense
1.1.1.433 T257A almost complete loss of activity Bifidobacterium catenulatum subsp. kashiwanohense
1.1.1.433 T257A site-directed mutagenesis, the mutant shows highly reduced activity for isethionate oxidation compared to wild-type enzyme Bifidobacterium catenulatum subsp. kashiwanohense

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.433 EDTA complete inhibition; complete loss of activity. Activity is recovered up to 85% by addition of Zn2+ and 15% by addition of Fe2+ Bifidobacterium catenulatum subsp. kashiwanohense

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.433 additional information
-
 additional information Michaelis-Menten kinetics Bifidobacterium catenulatum subsp. kashiwanohense
1.1.1.433 0.06
-
 NADH pH 10.0, 30°C, recombinant wild-type enzyme Bifidobacterium catenulatum subsp. kashiwanohense
1.1.1.433 0.06
-
 NADH pH 7.5, temperature not specified in the publication Bifidobacterium catenulatum subsp. kashiwanohense
1.1.1.433 0.08
-
 NAD+ pH 10.0, 30°C, recombinant wild-type enzyme Bifidobacterium catenulatum subsp. kashiwanohense
1.1.1.433 0.08
-
 NAD+ pH 10, temperature not specified in the publication Bifidobacterium catenulatum subsp. kashiwanohense
1.1.1.433 0.5
-
 2-sulfoacetaldehyde pH 10.0, 30°C, recombinant wild-type enzyme Bifidobacterium catenulatum subsp. kashiwanohense
1.1.1.433 0.5
-
 2-sulfoacetaldehyde pH 7.5, temperature not specified in the publication Bifidobacterium catenulatum subsp. kashiwanohense
1.1.1.433 0.73
-
 NADPH pH 10.0, 30°C, recombinant wild-type enzyme Bifidobacterium catenulatum subsp. kashiwanohense
1.1.1.433 0.77
-
 NADP+ pH 10.0, 30°C, recombinant wild-type enzyme Bifidobacterium catenulatum subsp. kashiwanohense
1.1.1.433 4.44
-
 isethionate pH 10.0, 30°C, recombinant wild-type enzyme Bifidobacterium catenulatum subsp. kashiwanohense
1.1.1.433 4.44
-
 isethionate pH 10, temperature not specified in the publication Bifidobacterium catenulatum subsp. kashiwanohense

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.433 Fe2+ activates, can partially substitute for Zn2+ Bifidobacterium catenulatum subsp. kashiwanohense
1.1.1.433 additional information catalytic activity of BkTauF is abolished by chelation with EDTA, and recovered up to 85% by addition of Zn2+ and 15% by addition of Fe2+, suggesting that the physiological metal cofactor for BkTauF is Zn2+ Bifidobacterium catenulatum subsp. kashiwanohense
1.1.1.433 Zn2+
-
 Bifidobacterium catenulatum subsp. kashiwanohense
1.1.1.433 Zn2+ required Bifidobacterium catenulatum subsp. kashiwanohense

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.433 83000
-
 gel filtration Bifidobacterium catenulatum subsp. kashiwanohense
1.1.1.433 83000
-
 recombinant enzyme, gel filtration Bifidobacterium catenulatum subsp. kashiwanohense

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.313 2-sulfoacetaldehyde + NADPH + H+ Chromohalobacter salexigens
-
 isethionate + NADP+
-
 r
1.1.1.313 2-sulfoacetaldehyde + NADPH + H+ Chromohalobacter salexigens DSM 3043
-
 isethionate + NADP+
-
 r
1.1.1.313 2-sulfoacetaldehyde + NADPH + H+ Chromohalobacter salexigens ATCC BAA-138
-
 isethionate + NADP+
-
 r
1.1.1.313 2-sulfoacetaldehyde + NADPH + H+ Chromohalobacter salexigens NCIMB 13768
-
 isethionate + NADP+
-
 r
1.1.1.313 2-sulfoacetaldehyde + NADPH + H+ Chromohalobacter salexigens 1H11
-
 isethionate + NADP+
-
 r
1.1.1.313 2-sulfoacetaldehyde + NADPH + H+ Chromohalobacter salexigens CIP 106854
-
 isethionate + NADP+
-
 r
1.1.1.433 2-sulfoacetaldehyde + NADH + H+ Bilophila wadsworthia
-
 isethionate + NAD+
-
 r
1.1.1.433 2-sulfoacetaldehyde + NADH + H+ Bifidobacterium catenulatum subsp. kashiwanohense
-
 isethionate + NAD+
-
 r
1.1.1.433 2-sulfoacetaldehyde + NADH + H+ Bilophila wadsworthia 3_1_6
-
 isethionate + NAD+
-
 r
1.1.1.433 2-sulfoacetaldehyde + NADH + H+ Bifidobacterium catenulatum subsp. kashiwanohense PV20-2
-
 isethionate + NAD+
-
 r
1.1.1.433 2-sulfoacetaldehyde + NADPH + H+ Bifidobacterium catenulatum subsp. kashiwanohense
-
 isethionate + NADP+
-
 r
1.1.1.433 2-sulfoacetaldehyde + NADPH + H+ Bifidobacterium catenulatum subsp. kashiwanohense PV20-2
-
 isethionate + NADP+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.313 Chromohalobacter salexigens Q1R183
-
-
1.1.1.313 Chromohalobacter salexigens 1H11 Q1R183
-
-
1.1.1.313 Chromohalobacter salexigens ATCC BAA-138 Q1R183
-
-
1.1.1.313 Chromohalobacter salexigens CIP 106854 Q1R183
-
-
1.1.1.313 Chromohalobacter salexigens DSM 3043 Q1R183
-
-
1.1.1.313 Chromohalobacter salexigens NCIMB 13768 Q1R183
-
-
1.1.1.433 Bifidobacterium catenulatum subsp. kashiwanohense
-
-
-
1.1.1.433 Bifidobacterium catenulatum subsp. kashiwanohense A0A0A7I0A5
-
-
1.1.1.433 Bifidobacterium catenulatum subsp. kashiwanohense PV20-2
-
-
-
1.1.1.433 Bifidobacterium catenulatum subsp. kashiwanohense PV20-2 A0A0A7I0A5
-
-
1.1.1.433 Bilophila wadsworthia E5Y946
-
-
1.1.1.433 Bilophila wadsworthia 3_1_6 E5Y946
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.433 recombinant His6-MBP-tagged enzyme from Escherichia coli strain BL21(DE3) by streptomycin sulfate, dialysis, amylose affinity chromatography, followed by MBP-tag cleavage through TEV protease, dialysis, and nickel affinity chromatography, anion exchange chromatography, and gel filtration Bifidobacterium catenulatum subsp. kashiwanohense

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.313 2-sulfoacetaldehyde + NADPH + H+
-
 Chromohalobacter salexigens isethionate + NADP+
-
 r
1.1.1.313 2-sulfoacetaldehyde + NADPH + H+
-
 Chromohalobacter salexigens DSM 3043 isethionate + NADP+
-
 r
1.1.1.313 2-sulfoacetaldehyde + NADPH + H+
-
 Chromohalobacter salexigens ATCC BAA-138 isethionate + NADP+
-
 r
1.1.1.313 2-sulfoacetaldehyde + NADPH + H+
-
 Chromohalobacter salexigens NCIMB 13768 isethionate + NADP+
-
 r
1.1.1.313 2-sulfoacetaldehyde + NADPH + H+
-
 Chromohalobacter salexigens 1H11 isethionate + NADP+
-
 r
1.1.1.313 2-sulfoacetaldehyde + NADPH + H+
-
 Chromohalobacter salexigens CIP 106854 isethionate + NADP+
-
 r
1.1.1.433 2-sulfoacetaldehyde + NADH + H+
-
 Bilophila wadsworthia isethionate + NAD+
-
 r
1.1.1.433 2-sulfoacetaldehyde + NADH + H+
-
 Bifidobacterium catenulatum subsp. kashiwanohense isethionate + NAD+
-
 r
1.1.1.433 2-sulfoacetaldehyde + NADH + H+ the reduction reaction direction is preferred by the enzyme Bifidobacterium catenulatum subsp. kashiwanohense isethionate + NAD+
-
 r
1.1.1.433 2-sulfoacetaldehyde + NADH + H+
-
 Bilophila wadsworthia 3_1_6 isethionate + NAD+
-
 r
1.1.1.433 2-sulfoacetaldehyde + NADH + H+
-
 Bifidobacterium catenulatum subsp. kashiwanohense PV20-2 isethionate + NAD+
-
 r
1.1.1.433 2-sulfoacetaldehyde + NADH + H+ the reduction reaction direction is preferred by the enzyme Bifidobacterium catenulatum subsp. kashiwanohense PV20-2 isethionate + NAD+
-
 r
1.1.1.433 2-sulfoacetaldehyde + NADPH + H+
-
 Bifidobacterium catenulatum subsp. kashiwanohense isethionate + NADP+
-
 r
1.1.1.433 2-sulfoacetaldehyde + NADPH + H+
-
 Bifidobacterium catenulatum subsp. kashiwanohense PV20-2 isethionate + NADP+
-
 r
1.1.1.433 3-hydroxypropane-1-sulfonate + NAD+
-
 Bifidobacterium catenulatum subsp. kashiwanohense ? + NADH + H+
-
 ?
1.1.1.433 3-hydroxypropane-1-sulfonate + NAD+ low activity Bifidobacterium catenulatum subsp. kashiwanohense ? + NADH + H+
-
 ?
1.1.1.433 3-hydroxypropane-1-sulfonate + NAD+ low activity Bifidobacterium catenulatum subsp. kashiwanohense PV20-2 ? + NADH + H+
-
 ?
1.1.1.433 3-hydroxypropane-1-sulfonate + NAD+
-
 Bifidobacterium catenulatum subsp. kashiwanohense PV20-2 ? + NADH + H+
-
 ?
1.1.1.433 3-hydroxypropionate + NAD+
-
 Bifidobacterium catenulatum subsp. kashiwanohense ? + NADH + H+
-
 ?
1.1.1.433 3-hydroxypropionate + NAD+
-
 Bifidobacterium catenulatum subsp. kashiwanohense PV20-2 ? + NADH + H+
-
 ?
1.1.1.433 3-hydroxypropionic acid + NAD+ moderate to low activity Bifidobacterium catenulatum subsp. kashiwanohense ? + NADH + H+
-
 ?
1.1.1.433 isethionate + NAD+
-
 Bifidobacterium catenulatum subsp. kashiwanohense 2-sulfoacetaldehyde + NADH + H+
-
 r
1.1.1.433 isethionate + NAD+
-
 Bifidobacterium catenulatum subsp. kashiwanohense PV20-2 2-sulfoacetaldehyde + NADH + H+
-
 r
1.1.1.433 isethionate + NADP+
-
 Bifidobacterium catenulatum subsp. kashiwanohense 2-sulfoacetaldehyde + NADPH + H+
-
 r
1.1.1.433 additional information substrate specificity, no activity with ethanol, ethylene glycol, and ethanolamine Bifidobacterium catenulatum subsp. kashiwanohense ?
-
-
1.1.1.433 additional information no substrates: ethanol, ethylene glycol, or ethanolamine Bifidobacterium catenulatum subsp. kashiwanohense ?
-
-
1.1.1.433 additional information substrate specificity, no activity with ethanol, ethylene glycol, and ethanolamine Bifidobacterium catenulatum subsp. kashiwanohense PV20-2 ?
-
-
1.1.1.433 additional information no substrates: ethanol, ethylene glycol, or ethanolamine Bifidobacterium catenulatum subsp. kashiwanohense PV20-2 ?
-
-

Subunits

EC Number Subunits Comment Organism
1.1.1.433 dimer 2 * 41000, SDS-PAGE Bifidobacterium catenulatum subsp. kashiwanohense
1.1.1.433 homodimer 2 * 41000, recombinant enzyme, SDS-PAGE Bifidobacterium catenulatum subsp. kashiwanohense

Synonyms

EC Number Synonyms Comment Organism
1.1.1.313 IsfD
-
 Chromohalobacter salexigens
1.1.1.433 AH68_00250
-
 Bifidobacterium catenulatum subsp. kashiwanohense
1.1.1.433 BkTauF
-
 Bifidobacterium catenulatum subsp. kashiwanohense
1.1.1.433 BwSarD
-
 Bilophila wadsworthia
1.1.1.433 sarD
-
 Bifidobacterium catenulatum subsp. kashiwanohense
1.1.1.433 sarD
-
 Bilophila wadsworthia
1.1.1.433 tauF
-
 Bifidobacterium catenulatum subsp. kashiwanohense
1.1.1.433 tauF
-
 Bilophila wadsworthia

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.433 30
-
 assay at Bifidobacterium catenulatum subsp. kashiwanohense

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.433 0.8
-
 NADP+ pH 10.0, 30°C, recombinant wild-type enzyme Bifidobacterium catenulatum subsp. kashiwanohense
1.1.1.433 0.97
-
 isethionate pH 10.0, 30°C, recombinant wild-type enzyme Bifidobacterium catenulatum subsp. kashiwanohense
1.1.1.433 0.97
-
 isethionate pH 10, temperature not specified in the publication Bifidobacterium catenulatum subsp. kashiwanohense
1.1.1.433 1.3
-
 NAD+ pH 10.0, 30°C, recombinant wild-type enzyme Bifidobacterium catenulatum subsp. kashiwanohense
1.1.1.433 1.3
-
 NAD+ pH 10, temperature not specified in the publication Bifidobacterium catenulatum subsp. kashiwanohense
1.1.1.433 18.67
-
 NADH pH 10.0, 30°C, recombinant wild-type enzyme Bifidobacterium catenulatum subsp. kashiwanohense
1.1.1.433 18.67
-
 NADH pH 7.5, temperature not specified in the publication Bifidobacterium catenulatum subsp. kashiwanohense
1.1.1.433 18.99
-
 NADPH pH 10.0, 30°C, recombinant wild-type enzyme Bifidobacterium catenulatum subsp. kashiwanohense
1.1.1.433 22.83
-
 2-sulfoacetaldehyde pH 10.0, 30°C, recombinant wild-type enzyme Bifidobacterium catenulatum subsp. kashiwanohense
1.1.1.433 22.83
-
 2-sulfoacetaldehyde pH 7.5, temperature not specified in the publication Bifidobacterium catenulatum subsp. kashiwanohense

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.433 7.5
-
 sulfoacetaldehyde reduction Bifidobacterium catenulatum subsp. kashiwanohense
1.1.1.433 10
-
 assay at Bifidobacterium catenulatum subsp. kashiwanohense
1.1.1.433 10
-
 isethionate oxidation Bifidobacterium catenulatum subsp. kashiwanohense

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.313 NADP+
-
 Chromohalobacter salexigens
1.1.1.313 NADPH
-
 Chromohalobacter salexigens
1.1.1.433 additional information NADH/NAD+ is preferred before NADPH/NADP+ by the enzyme Bifidobacterium catenulatum subsp. kashiwanohense
1.1.1.433 additional information enzyme accepts NADP+/NADPH, reaction of EC 1.1.1.313 Bifidobacterium catenulatum subsp. kashiwanohense
1.1.1.433 NAD+
-
 Bilophila wadsworthia
1.1.1.433 NAD+
-
 Bifidobacterium catenulatum subsp. kashiwanohense
1.1.1.433 NADH
-
 Bilophila wadsworthia
1.1.1.433 NADH
-
 Bifidobacterium catenulatum subsp. kashiwanohense
1.1.1.433 NADH preferred cofactor Bifidobacterium catenulatum subsp. kashiwanohense
1.1.1.433 NADP+
-
 Bifidobacterium catenulatum subsp. kashiwanohense
1.1.1.433 NADPH
-
 Bifidobacterium catenulatum subsp. kashiwanohense

General Information

EC Number General Information Comment Organism
1.1.1.313 evolution the enzyme sulfoacetaldehyde reductase IsfD belongs to the short-chain dehydrogenase/reductase (SDR) family Chromohalobacter salexigens
1.1.1.313 metabolism in the pathway, taurine is imported by a taurine ABC transporter (TauABC), and converted into sulfoacetaldehyde by taurine:oxoglutarate aminotransferase (Toa), generating glutamate as an intermediate for nitrogen metabolism. The NADPH-dependent sulfoacetaldehyde reductase (IsfD), belonging to the SDR family, generates isethionate as a waste produce, which is exported by the putative isethionate exporter (IsfE) Chromohalobacter salexigens
1.1.1.313 physiological function hydroxyethylsulfonate (isethionate (Ise)) is generated by the sulfoacetaldehyde reductases from human gut bacteria. Isethionate is thought to be derived from aminoethylsulfonate (taurine), as a byproduct of taurine nitrogen assimilation by certain anaerobic bacteria inhabiting the taurine-rich mammalian gut Chromohalobacter salexigens
1.1.1.433 evolution sulfoacetaldehyde reductase from the human gut fermenting bacterium Bifidobacterium kashiwanohense (BkTauF) belongs to the M-ADH family, but is distantly related to BwSarD (28% sequence identity), a sulfoacetaldehyde reductase from human gut sulfite-reducing bacterium Bilophila wadsworthia belonging to the metal-dependent alcohol dehydrogenase superfamily (M-ADH). Conservation of active site residues (D192, Q196, F252, T257, H261, F265 and H275) in close homologues of BkTauF Bifidobacterium catenulatum subsp. kashiwanohense
1.1.1.433 evolution sulfoacetaldehyde reductase SarD, from human gut sulfite-reducing bacterium Bilophila wadsworthia belongs to the metal-dependent alcohol dehydrogenase superfamily (M-ADH). Sulfoacetaldehyde reductase from the human gut fermenting bacterium Bifidobacterium kashiwanohense (BkTauF) belongs to the M-ADH family, but is distantly related to BwSarD (28% sequence identity) Bilophila wadsworthia
1.1.1.433 metabolism the enzyme is involved in a pathway for taurine dissimilation, in which isethionate is generated as an intermediate, and further degraded to acetate and H2S instead of being secreted Bilophila wadsworthia
1.1.1.433 additional information the BktauF structure and sequence shows conservation in secondary structure, and metal-coordinating residues except that Gln196 is atypical in the M-ADH family, with His being more common at that position. The putative isethionate-binding site adjacent to the catalytic Zn2+ is very open, which precluded molecular docking, active site structure analysis, overview. The position of isethionate is constrained by the requirements of the M-ADH catalytic mechanism, which requires coordination of the hydroxyl O-atom to Zn2+, and hydride transfer from C1 of isethionate to C4 of the NAD+. Phe252, Thr257 and Phe265 surrounding the active-site cavity are identified as potential substrate-interacting residues Bifidobacterium catenulatum subsp. kashiwanohense
1.1.1.433 physiological function hydroxyethylsulfonate (isethionate (Ise)) is generated by the sulfoacetaldehyde reductases from human gut bacteria. Isethionate is thought to be derived from aminoethylsulfonate (taurine), as a byproduct of taurine nitrogen assimilation by certain anaerobic bacteria inhabiting the taurine-rich mammalian gut. Sulfoacetaldehyde reductases in Bifidobacteria have a possible role in isethionate production as a byproduct of taurine nitrogen assimilation Bilophila wadsworthia
1.1.1.433 physiological function hydroxyethylsulfonate (isethionate (Ise)) is generated by the sulfoacetaldehyde reductases from human gut bacteria. Isethionate is thought to be derived from aminoethylsulfonate (taurine), as a byproduct of taurine nitrogen assimilation by certain anaerobic bacteria inhabiting the taurine-rich mammalian gut. Sulfoacetaldehyde reductases in Bifidobacteria have a possible role in isethionate production as a byproduct of taurine nitrogen assimilation Bifidobacterium catenulatum subsp. kashiwanohense

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.433 0.22
-
 isethionate pH 10.0, 30°C, recombinant wild-type enzyme Bifidobacterium catenulatum subsp. kashiwanohense
1.1.1.433 0.22
-
 isethionate pH 10, temperature not specified in the publication Bifidobacterium catenulatum subsp. kashiwanohense
1.1.1.433 1.04
-
 NADP+ pH 10.0, 30°C, recombinant wild-type enzyme Bifidobacterium catenulatum subsp. kashiwanohense
1.1.1.433 15.8
-
 NAD+ pH 10, temperature not specified in the publication Bifidobacterium catenulatum subsp. kashiwanohense
1.1.1.433 16.25
-
 NAD+ pH 10.0, 30°C, recombinant wild-type enzyme Bifidobacterium catenulatum subsp. kashiwanohense
1.1.1.433 26.01
-
 NADPH pH 10.0, 30°C, recombinant wild-type enzyme Bifidobacterium catenulatum subsp. kashiwanohense
1.1.1.433 45.66
-
 2-sulfoacetaldehyde pH 10.0, 30°C, recombinant wild-type enzyme Bifidobacterium catenulatum subsp. kashiwanohense
1.1.1.433 46.1
-
 2-sulfoacetaldehyde pH 7.5, temperature not specified in the publication Bifidobacterium catenulatum subsp. kashiwanohense
1.1.1.433 301
-
 NADH pH 7.5, temperature not specified in the publication Bifidobacterium catenulatum subsp. kashiwanohense
1.1.1.433 311.17
-
 NADH pH 10.0, 30°C, recombinant wild-type enzyme Bifidobacterium catenulatum subsp. kashiwanohense