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Literature summary extracted from

  • Sakurai, H.; Yokota, A.; Sumita, Y.; Mori, Y.; Matsui, H.; Tomita, F.
    Metabolism of DFA III by Arthrobacter sp. H65-7 Purification and properties of a DFA III hydrolysis enzyme (DFA IIIase) (1997), Biosci. Biotechnol. Biochem., 61, 989-993 .
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.178 12.5
-
 alpha-D-fructofuranose-beta-D-fructofuranose 2',1:2,3'-dianhydride at pH 6.0 and 45°C Arthrobacter sp. H65-7
4.2.1.178 30.9
-
 Inulobiose at pH 6.0 and 45°C Arthrobacter sp. H65-7

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.178 125000
-
 gel filtration Arthrobacter sp. H65-7

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.178 alpha-D-fructofuranose-beta-D-fructofuranose 2',1:2,3'-dianhydride + H2O Arthrobacter sp. H65-7 highest activity inulobiose
-
 r
4.2.1.178 D-fructose + H2O Arthrobacter sp. H65-7 very low activity ?
-
 ?
4.2.1.178 inulin + H2O Arthrobacter sp. H65-7 high activity ?
-
 ?
4.2.1.178 inulobiose Arthrobacter sp. H65-7 low activity alpha-D-fructofuranose-beta-D-fructofuranose 2',1:2,3'-dianhydride + H2O
-
 r
4.2.1.178 levan + H2O Arthrobacter sp. H65-7 very low activity ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.178 Arthrobacter sp. H65-7
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.178 ammonium sulfate precipitation and chromatographies on DEAE-Toyopearl 650M, butyl-Sepharose 4B, TSKgel G3000SW and Biophoresis III Arthrobacter sp. H65-7

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.178 0.53
-
 cell-free extract, at pH 6.0 and 45°C Arthrobacter sp. H65-7
4.2.1.178 19.2
-
 purified enzyme, at pH 6.0 and 45°C Arthrobacter sp. H65-7

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.178 alpha-D-fructofuranose-beta-D-fructofuranose 2',1:2,3'-dianhydride + H2O highest activity Arthrobacter sp. H65-7 inulobiose
-
 r
4.2.1.178 D-fructose + H2O very low activity Arthrobacter sp. H65-7 ?
-
 ?
4.2.1.178 inulin + H2O high activity Arthrobacter sp. H65-7 ?
-
 ?
4.2.1.178 inulobiose low activity Arthrobacter sp. H65-7 alpha-D-fructofuranose-beta-D-fructofuranose 2',1:2,3'-dianhydride + H2O
-
 r
4.2.1.178 levan + H2O very low activity Arthrobacter sp. H65-7 ?
-
 ?
4.2.1.178 additional information no activity with di-D-fructofuranose 6,2' : 2,6'-dianhydride, levanbiose, xylan, starch, cellulose, raffinose, sucrose, maltose, lactose, cellobiose, D-glucose, D-xylose, glycerol, and acetate Arthrobacter sp. H65-7 ?
-
-

Subunits

EC Number Subunits Comment Organism
4.2.1.178 homodimer 2 * 61000, SDS-PAGE Arthrobacter sp. H65-7

Synonyms

EC Number Synonyms Comment Organism
4.2.1.178 DFA III hydrolysis enzyme
-
 Arthrobacter sp. H65-7
4.2.1.178 DFA IIIase
-
 Arthrobacter sp. H65-7

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.178 45
-
-
 Arthrobacter sp. H65-7

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.1.178 60 75 the enzyme is stable up to 60°C with more than 80% of the enzyme activity remaining after 15 min. More than 80% of the activity is kept up to 75°C in the cell-free extracts Arthrobacter sp. H65-7

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.178 6
-
-
 Arthrobacter sp. H65-7

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.2.1.178 4.5 10 more than 80% of the enzyme activity remains between pH 4.5 and 10.0 after 18 h incubation Arthrobacter sp. H65-7