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Literature summary extracted from

  • Takeda, K.; Igarashi, K.; Yoshida, M.; Nakamura, N.
    Discovery of a novel quinohemoprotein from a eukaryote and its application in electrochemical devices (2020), Bioelectrochemistry, 131, 107372 .
    View publication on PubMed

Application

EC Number Application Comment Organism
1.1.2.B5 diagnostics bioelectrochemical applications (an amperometric biosensor) in electrochemical devices, based on direct electron transfer reactions. Development of a biosensor to detect urinary L-fucose Coprinopsis cinerea

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.2.B5 expression in Pichia pastoris Coprinopsis cinerea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.2.B5 98000
-
 SDS-PAGE Coprinopsis cinerea

Organism

EC Number Organism UniProt Comment Textmining
1.1.2.B5 Coprinopsis cinerea
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.2.B5 additional information no activity toward common monosaccharides, such as D-glucose, D-fructose, and cellobiose Coprinopsis cinerea ?
-
-

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.2.B5 6
-
 optimum pH for theoxidation reaction of L-fucose, the electron is directly transferred from the PQQ domain to the electron acceptor. At 8.5 the electron is transferred via the cytochrome domain. This phenomenon indicates that inter-domain electron transfer (IET) is strongly dependent on pH Coprinopsis cinerea

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.2.B5 heme quinohemoprotein Coprinopsis cinerea
1.1.2.B5 pyrroloquinoline quinone pyrroloquinoline quinone-dependent enzyme. The enzyme binds to pyrroloquinoline quinone at a ratio of 1: 1 Coprinopsis cinerea

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.1.2.B5 Coprinopsis cinerea calculated from sequence
-
 7.7