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Literature summary extracted from

  • Wiezel, G.A.; Rustiguel, J.K.; Morgenstern, D.; Zoccal, K.F.; Faccioli, L.H.; Nonato, M.C.; Ueberheide, B.; Arantes, E.C.
    Insights into the structure, function and stability of bordonein-L, the first L-amino acid oxidase from Crotalus durissus terrificus snake venom (2019), Biochimie, 163, 33-49 .
    View publication on PubMed

Application

EC Number Application Comment Organism
1.4.3.2 medicine the enzyme exhibits cytotoxicity against fibroblast cell line and kills Leishmania amazonensis promastigotes, intensified by substrate addition Crotalus durissus terrificus

General Stability

EC Number General Stability Organism
1.4.3.2 the enzyme tends to destabilize from neutral to basic pHs and in presence of mono/bivalent ions and it is highly stabilized by acid pHs and its substrates Crotalus durissus terrificus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.3.2 0.14
-
L-phenylalanine pH not specified in the publication, temperature not specified in the publication Crotalus durissus terrificus
1.4.3.2 0.48
-
L-tryptophan pH not specified in the publication, temperature not specified in the publication Crotalus durissus terrificus
1.4.3.2 0.58
-
L-leucine pH not specified in the publication, temperature not specified in the publication Crotalus durissus terrificus
1.4.3.2 0.97
-
L-methionine pH not specified in the publication, temperature not specified in the publication Crotalus durissus terrificus
1.4.3.2 3.9
-
L-isoleucine pH not specified in the publication, temperature not specified in the publication Crotalus durissus terrificus

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.2 Crotalus durissus terrificus C0HJE7
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.4.3.2 glycoprotein sequence contains one N-glycosylation site and two disulfide bonds, protein shows about 5% carbohydrate content Crotalus durissus terrificus

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.3.2 purification by cationic exchange, size exclusion, and glycoprotein affinity Crotalus durissus terrificus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.4.3.2 venom
-
Crotalus durissus terrificus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.3.2 5.44
-
pH not specified in the publication, temperature not specified in the publication Crotalus durissus terrificus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.2 L-isoleucine + H2O + O2
-
Crotalus durissus terrificus 3-methyl-2-oxopentanoate + NH3 + H2O2
-
?
1.4.3.2 L-leucine + H2O + O2
-
Crotalus durissus terrificus 2-oxo-4-methylpentanoate + NH3 + H2O2
-
?
1.4.3.2 L-methionine + H2O + O2
-
Crotalus durissus terrificus 4-methylthio-2-oxobutanoate + NH3 + H2O2
-
?
1.4.3.2 L-phenylalanine + O2
-
Crotalus durissus terrificus phenylpyruvate + NH3 + H2O2
-
?
1.4.3.2 L-tryptophan + H2O + O2
-
Crotalus durissus terrificus indole-3-pyruvate + NH3 + H2O2
-
?
1.4.3.2 additional information enzyme shows highest enzymatic activities upon the following L-hydrophobic amino acids: Met, Leu, Trp, Phe and Ile. L-amino acids not oxidized by bordonein-L include Lys, Ser, Thr, Pro, Asp, Gly, Tyr, Cys and Glu. Low activity is observed with His and Arg. Gln, Val, Asn and Ala are even more poorly oxidized by bordonein-L Crotalus durissus terrificus ?
-
-

Subunits

EC Number Subunits Comment Organism
1.4.3.2 ? x * 113833, calculated from sequence Crotalus durissus terrificus

Synonyms

EC Number Synonyms Comment Organism
1.4.3.2 bordonein-L
-
Crotalus durissus terrificus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.4.3.2 3421
-
L-isoleucine pH not specified in the publication, temperature not specified in the publication Crotalus durissus terrificus
1.4.3.2 3483
-
L-phenylalanine pH not specified in the publication, temperature not specified in the publication Crotalus durissus terrificus
1.4.3.2 3720
-
L-tryptophan pH not specified in the publication, temperature not specified in the publication Crotalus durissus terrificus
1.4.3.2 5965
-
L-leucine pH not specified in the publication, temperature not specified in the publication Crotalus durissus terrificus
1.4.3.2 7284
-
L-methionine pH not specified in the publication, temperature not specified in the publication Crotalus durissus terrificus

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.3.2 FAD
-
Crotalus durissus terrificus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.4.3.2 Crotalus durissus terrificus calculated from sequence
-
8.2

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.4.3.2 900
-
L-isoleucine pH not specified in the publication, temperature not specified in the publication Crotalus durissus terrificus
1.4.3.2 7500
-
L-methionine pH not specified in the publication, temperature not specified in the publication Crotalus durissus terrificus
1.4.3.2 7800
-
L-tryptophan pH not specified in the publication, temperature not specified in the publication Crotalus durissus terrificus
1.4.3.2 10000
-
L-leucine pH not specified in the publication, temperature not specified in the publication Crotalus durissus terrificus
1.4.3.2 25000
-
L-phenylalanine pH not specified in the publication, temperature not specified in the publication Crotalus durissus terrificus