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Literature summary extracted from

  • Hartuti, E.D.; Inaoka, D.K.; Komatsuya, K.; Miyazaki, Y.; Miller, R.J.; Xinying, W.; Sadikin, M.; Prabandari, E.E.; Waluyo, D.; Kuroda, M.; Amalia, E.; Matsuo, Y.; Nugroho, N.B.; Saimoto, H.; Pramisandi, A.; Watanabe, Y.I.; Mori, M.; Shiomi, K.; Balogun, E.O.; Shiba, T.; Harada, S.; Nozaki, T.; Kita, K.
    Biochemical studies of membrane bound Plasmodium falciparum mitochondrial L-malate quinone oxidoreductase, a potential drug target (2018), Biochim. Biophys. Acta Bioenerg., 1859, 191-200 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.5.4 overexpression in an Escherichia coli BL21Star(DE3) Plasmodium falciparum

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.5.4 ferulenol inhibits parasite growth and shows strong synergism in combination with atovaquone, an anti-malarial and bc1 complex inhibitor Plasmodium falciparum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.5.4 0.0062
-
 decylubiquinone pH 7.0, 37°C Plasmodium falciparum
1.1.5.4 5.99
-
 (S)-malate pH 7.0, 37°C Plasmodium falciparum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.5.4 membrane membrane bound Plasmodium falciparum 16020
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.5.4 (S)-malate + ubiquinone Plasmodium falciparum the enzyme is involved in three pathways (mitochondrial electron transport chain, the tricarboxylic acid cycle and the fumarate cycle) oxaloacetate + ubiquinol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.5.4 Plasmodium falciparum C6KT09 isolate 3D7
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.5.4 (S)-malate + decylubiquinone
-
 Plasmodium falciparum oxaloacetate + decylubiquinol
-
 ?
1.1.5.4 (S)-malate + ubiquinone the enzyme is involved in three pathways (mitochondrial electron transport chain, the tricarboxylic acid cycle and the fumarate cycle) Plasmodium falciparum oxaloacetate + ubiquinol
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.5.4 L-malate:quinone oxidoreductase
-
 Plasmodium falciparum
1.1.5.4 PfMQO
-
 Plasmodium falciparum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.5.4 37
-
-
 Plasmodium falciparum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.5.4 25 40 25-30°C: about 50% of maximal activity, 40°C: about 90% of maximal activity Plasmodium falciparum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.5.4 7.3
-
-
 Plasmodium falciparum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.5.4 6.8 8.2 no significant difference in activity from pH 6.8 to 8.2 Plasmodium falciparum

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.1.5.4 0.00925
-
 pH 7.0, 37°C Plasmodium falciparum ferulenol

General Information

EC Number General Information Comment Organism
1.1.5.4 drug target the enzyme is a potential antimalarial drug target against the apicomplexan parasite Plasmodium falciparum, because the enzyme is absent in humans. Ferulenol inhibits parasite growth and shows strong synergism in combination with atovaquone, an anti-malarial and bc1 complex inhibitor Plasmodium falciparum
1.1.5.4 metabolism the enzyme is involved in three pathways (mitochondrial electron transport chain, the tricarboxylic acid cycle and the fumarate cycle) Plasmodium falciparum
1.1.5.4 physiological function the enzyme is essential for parasite survival, at least, in the intra-erythrocytic asexual stage Plasmodium falciparum