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Literature summary extracted from

  • Abrera, A.; Chang, H.; Kracher, D.; Ludwig, R.; Haltrich, D.
    Characterization of pyranose oxidase variants for bioelectrocatalytic applications (2020), Biochim. Biophys. Acta, 1868, 140335 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.3.10 expression in Escherichia coli Trametes ochracea

Protein Variants

EC Number Protein Variants Comment Organism
1.1.3.10 L545C pre-steady-state kinetics of the oxidative half-reaction show higher kinetic constants as compared to the wild-type. The variant immobilized on glassy carbon electrodes displays high catalytic efficiency. L545C performs best among the variants studied Trametes ochracea
1.1.3.10 L547R pre-steady-state kinetics of the oxidative half-reaction show higher kinetic constants as compared to the wild-type. The variant immobilized on glassy carbon electrodes displays high catalytic efficiency Trametes ochracea
1.1.3.10 Q448H pre-steady-state kinetics of the oxidative half-reaction show higher kinetic constants as compared to the wild-type. The variant immobilized on glassy carbon electrodes displays high catalytic efficiency Trametes ochracea
1.1.3.10 T166R pre-steady-state kinetics of the oxidative half-reaction show higher kinetic constants as compared to the wild-type. The variant immobilized on glassy carbon electrodes displays high catalytic efficiency Trametes ochracea

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.10 Trametes ochracea Q7ZA32
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.10 D-glucose + 1,4-benzoquinone
-
 Trametes ochracea 2-dehydro-D-glucose + hydroquinone
-
 ?
1.1.3.10 D-glucose + 2,6-dichlorophenolindophenol
-
 Trametes ochracea 2-dehydro-D-glucose + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.3.10 D-glucose + ferricenium ion
-
 Trametes ochracea 2-dehydro-D-glucose + ferrocenium ion
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.3.10 ? x * 65000, SDS-PAGE Trametes ochracea

Synonyms

EC Number Synonyms Comment Organism
1.1.3.10 P2O
-
 Trametes ochracea