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Literature summary extracted from

  • Kadowaki, M.; Higasi, P.; de Godoy, M.; de Araujo, E.; Godoy, A.; Prade, R.; Polikarpov, I.
    Enzymatic versatility and thermostability of a new aryl-alcohol oxidase from Thermothelomyces thermophilus M77 (2020), Biochim. Biophys. Acta, 1864, 129681 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.3.7 Ca2+ 1 mM, 119% of initial activity Thermothelomyces thermophilus
1.1.3.7 Co2+ 1 mM, 111% of initial activity Thermothelomyces thermophilus
1.1.3.7 K+ 1 mM, 106% of initial activity Thermothelomyces thermophilus
1.1.3.7 Sr2+ 1 mM, 106% of initial activity Thermothelomyces thermophilus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.3.7 expression in Aspergillus nidulans Thermothelomyces thermophilus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.3.7 to 2.6 A resolution Thermothelomyces thermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.7 0.47
-
 veratryl alcohol pH 6, 30°C Thermothelomyces thermophilus
1.1.3.7 0.49
-
 cinnamyl alcohol pH 6, 30°C Thermothelomyces thermophilus
1.1.3.7 1.03
-
 anisyl alcohol pH 6, 30°C Thermothelomyces thermophilus
1.1.3.7 13.02
-
 5-hydroxymethylfurfural pH 6, 30°C Thermothelomyces thermophilus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.3.7 Ba2+ 1 mM, 62% of initial activity Thermothelomyces thermophilus
1.1.3.7 Cu2+ 1 mM, 49% of initial activity Thermothelomyces thermophilus
1.1.3.7 ethanol 20%, 93% of initial activity Thermothelomyces thermophilus
1.1.3.7 Fe2+ 1 mM, 93% of initial activity Thermothelomyces thermophilus
1.1.3.7 Mg2+ 1 mM, 91of initial activity Thermothelomyces thermophilus
1.1.3.7 SDS 1%,81% of initial activity Thermothelomyces thermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.3.7 79500
-
 MALDI-TOF mass spectrometry Thermothelomyces thermophilus

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.7 Thermothelomyces thermophilus G2PZJ2
-
-
1.1.3.7 Thermothelomyces thermophilus DSM 1799 G2PZJ2
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.1.3.7 glycoprotein enzyme shows about 14% glycosylation Thermothelomyces thermophilus

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.3.7 recombinant protein Thermothelomyces thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.7 5-hydroxymethylfurfural + O2
-
 Thermothelomyces thermophilus 2,5-diformylfuran + 5-formylfuran-2-carboxylic acid + H2O2
-
 ?
1.1.3.7 5-hydroxymethylfurfural + O2
-
 Thermothelomyces thermophilus DSM 1799 2,5-diformylfuran + 5-formylfuran-2-carboxylic acid + H2O2
-
 ?
1.1.3.7 anisyl alcohol + O2
-
 Thermothelomyces thermophilus anisyl aldehyde + H2O2
-
 ?
1.1.3.7 anisyl alcohol + O2
-
 Thermothelomyces thermophilus DSM 1799 anisyl aldehyde + H2O2
-
 ?
1.1.3.7 cinnamyl alcohol + O2
-
 Thermothelomyces thermophilus cinnamyl aldehyde + H2O2
-
 ?
1.1.3.7 cinnamyl alcohol + O2
-
 Thermothelomyces thermophilus DSM 1799 cinnamyl aldehyde + H2O2
-
 ?
1.1.3.7 additional information no substrates: glucose, cellobiose, methanol Thermothelomyces thermophilus ?
-
-
1.1.3.7 additional information no substrates: glucose, cellobiose, methanol Thermothelomyces thermophilus DSM 1799 ?
-
-
1.1.3.7 veratryl alcohol + O2
-
 Thermothelomyces thermophilus veratraldehyde + H2O2
-
 ?
1.1.3.7 veratryl alcohol + O2
-
 Thermothelomyces thermophilus DSM 1799 veratraldehyde + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.3.7 monomer 1 * 68300, calculated from sequence Thermothelomyces thermophilus

Synonyms

EC Number Synonyms Comment Organism
1.1.3.7 GMC oxidoreductase-like protein
-
 Thermothelomyces thermophilus
1.1.3.7 MYCTH_2299749
-
 Thermothelomyces thermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.3.7 50
-
-
 Thermothelomyces thermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.3.7 60
-
 half-life 10 min in absence of Ca2+, in presence of Ca2+ activity is maintained for 2 h Thermothelomyces thermophilus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.3.7 0.0008
-
 5-hydroxymethylfurfural pH 6, 30°C Thermothelomyces thermophilus
1.1.3.7 0.0053
-
 veratryl alcohol pH 6, 30°C Thermothelomyces thermophilus
1.1.3.7 0.0069
-
 cinnamyl alcohol pH 6, 30°C Thermothelomyces thermophilus
1.1.3.7 0.0075
-
 anisyl alcohol pH 6, 30°C Thermothelomyces thermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.3.7 7
-
-
 Thermothelomyces thermophilus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.3.7 FAD molar ration of FAD to purified enzyme is 0.69 Thermothelomyces thermophilus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.3.7 0.001
-
 5-hydroxymethylfurfural pH 6, 30°C Thermothelomyces thermophilus
1.1.3.7 0.008
-
 anisyl alcohol pH 6, 30°C Thermothelomyces thermophilus
1.1.3.7 0.011
-
 veratryl alcohol pH 6, 30°C Thermothelomyces thermophilus
1.1.3.7 0.014
-
 cinnamyl alcohol pH 6, 30°C Thermothelomyces thermophilus