EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.4.3.3 | Y228L/R283G | (R)-alpha-methylbenzylamine as neutral amine is substrate of mutant Y228L/R283G, while an active-site residue, likely Tyr224, must be uncharged for productive binding. The oxidative half-reaction is unperturbed by the mutation and with flavin oxidation preceding product release | Sus scrofa |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.4.3.3 | 2.9 | - |
(R)-alpha-methylbenzylamine | mutant Y228L/R283G, pH 8, 25°C. The kcat/Km profile exhibits a maximum at pH 10 | Sus scrofa | |
1.4.3.3 | 5 | 20 | O2 | mutant Y228L/R283G, pH 8, 25°C | Sus scrofa |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.4.3.3 | Sus scrofa | P00371 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.4.3.3 | (R)-alpha-methylbenzylamine + H2O + O2 | substrate of mutant Y228L/R283G, the neutral amine is the substrate | Sus scrofa | alpha-methylbenzaldehyde + NH3 + H2O2 | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.4.3.3 | DAO | - |
Sus scrofa |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.4.3.3 | 88 | - |
(R)-alpha-methylbenzylamine | mutant Y228L/R283G, pH 8, 25°C | Sus scrofa |