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Literature summary extracted from

  • Warlick, B.; Evans, B.; Erb, T.; Ramagopal, U.; Sriram, J.; Imker, H.; Sauder, J.; Bonanno, J.; Burley, S.; Tabita, F.; Almo, S.; Sweedler, J.; Gerlt, J.
    1-methylthio-d-xylulose 5-phosphate methylsulfurylase A novel route to 1-deoxy-d-xylulose 5-phosphate in Rhodospirillum rubrum (2012), Biochemistry, 51, 8324-8326 .
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
2.8.4.6 C121A inactive Rhodospirillum rubrum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8.4.6 0.039
-
 L-dithiothreitol pH not specified in the publication, temperature not specified in the publication Rhodospirillum rubrum
2.8.4.6 0.042
-
 glutathione pH not specified in the publication, temperature not specified in the publication Rhodospirillum rubrum
2.8.4.6 0.46
-
 dihydrolipoate pH not specified in the publication, temperature not specified in the publication Rhodospirillum rubrum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.4.6 S-methyl-1-thio-D-xylulose 5-phosphate + glutathione Rhodospirillum rubrum
-
 1-deoxy-D-xylulose 5-phosphate + S-(methylsulfanyl)glutathione
-
 ?
2.8.4.6 S-methyl-1-thio-D-xylulose 5-phosphate + glutathione Rhodospirillum rubrum DSM 467
-
 1-deoxy-D-xylulose 5-phosphate + S-(methylsulfanyl)glutathione
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.8.4.6 Rhodospirillum rubrum Q2RSU5
-
-
2.8.4.6 Rhodospirillum rubrum DSM 467 Q2RSU5
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.4.6 additional information in vivo, acceptor is glutathione, in vitro, glutathione can be substituted by dithiothreitol or dihydrolipoate Rhodospirillum rubrum ?
-
-
2.8.4.6 additional information in vivo, acceptor is glutathione, in vitro, glutathione can be substituted by dithiothreitol or dihydrolipoate Rhodospirillum rubrum DSM 467 ?
-
-
2.8.4.6 S-methyl-1-thio-D-xylulose 5-phosphate + dihydrolipoate
-
 Rhodospirillum rubrum 1-deoxy-D-xylulose 5-phosphate + S-(methylsulfanyl)dihydrolipoate
-
 ?
2.8.4.6 S-methyl-1-thio-D-xylulose 5-phosphate + dihydrolipoate
-
 Rhodospirillum rubrum DSM 467 1-deoxy-D-xylulose 5-phosphate + S-(methylsulfanyl)dihydrolipoate
-
 ?
2.8.4.6 S-methyl-1-thio-D-xylulose 5-phosphate + glutathione
-
 Rhodospirillum rubrum 1-deoxy-D-xylulose 5-phosphate + S-(methylsulfanyl)glutathione
-
 ?
2.8.4.6 S-methyl-1-thio-D-xylulose 5-phosphate + glutathione
-
 Rhodospirillum rubrum DSM 467 1-deoxy-D-xylulose 5-phosphate + S-(methylsulfanyl)glutathione
-
 ?
2.8.4.6 S-methyl-1-thio-D-xylulose 5-phosphate + L-dithiothreitol
-
 Rhodospirillum rubrum 1-deoxy-D-xylulose 5-phosphate + S-(methylsulfanyl)dithiothreitol
-
 ?
2.8.4.6 S-methyl-1-thio-D-xylulose 5-phosphate + L-dithiothreitol
-
 Rhodospirillum rubrum DSM 467 1-deoxy-D-xylulose 5-phosphate + S-(methylsulfanyl)dithiothreitol
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.8.4.6 MTXu 5-P methylsulfurylase
-
 Rhodospirillum rubrum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.8.4.6 0.19
-
 glutathione pH not specified in the publication, temperature not specified in the publication Rhodospirillum rubrum
2.8.4.6 4.3
-
 dihydrolipoate pH not specified in the publication, temperature not specified in the publication Rhodospirillum rubrum
2.8.4.6 7.8
-
 L-dithiothreitol pH not specified in the publication, temperature not specified in the publication Rhodospirillum rubrum

General Information

EC Number General Information Comment Organism
2.8.4.6 metabolism an active site Cys residue displaces the enolate of 1-deoxy-D-xylulose 5-phosphate from 1-methylthio-D-xylulose 5-phosphate, generating a methyl disulfide intermediate Rhodospirillum rubrum
2.8.4.6 physiological function enzyme belongs to the cupin superfamily and participates in an anaerobic salvage pathway that restores methionine from S-methyl-5'-thioadenosine Rhodospirillum rubrum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.8.4.6 4.5
-
 glutathione pH not specified in the publication, temperature not specified in the publication Rhodospirillum rubrum
2.8.4.6 93
-
 dihydrolipoate pH not specified in the publication, temperature not specified in the publication Rhodospirillum rubrum
2.8.4.6 200
-
 L-dithiothreitol pH not specified in the publication, temperature not specified in the publication Rhodospirillum rubrum