Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Neubauer, A.; Walter, M.; Buchholz, K.
    Formation of inulobiose from difructoseanhydride III catalysed by a lysate from Arthrobacter ureafaciens ATCC 21124 (2000), Biocatal. Biotransform., 18, 443-455 .
No PubMed abstract available

General Stability

EC Number General Stability Organism
4.2.1.178 product formation can be enhanced by buffering with 0.1 M Na-borate/HCl buffer Paenarthrobacter ureafaciens

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.178 difructoseanhydride III inulobiose formation is slightly inhibited at difructoseanhydride concentrations of more than 75-100g/l (0.23-0.31 M) Paenarthrobacter ureafaciens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.178 22
-
 Inulobiose at pH 5.5 and 60°C Paenarthrobacter ureafaciens
4.2.1.178 31
-
 alpha-D-fructofuranose-beta-D-fructofuranose-1,2':2,3'-dianhydride at pH 5.5 and 60°C Paenarthrobacter ureafaciens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.178 alpha-D-fructofuranose-beta-D-fructofuranose-1,2':2,3'-dianhydride + H2O Paenarthrobacter ureafaciens
-
 inulobiose
-
 r
4.2.1.178 alpha-D-fructofuranose-beta-D-fructofuranose-1,2':2,3'-dianhydride + H2O Paenarthrobacter ureafaciens ATCC 21124
-
 inulobiose
-
 r
4.2.1.178 inulobiose Paenarthrobacter ureafaciens
-
 alpha-D-fructofuranose-beta-D-fructofuranose-1,2':2,3'-dianhydride + H2O
-
 r
4.2.1.178 inulobiose Paenarthrobacter ureafaciens ATCC 21124
-
 alpha-D-fructofuranose-beta-D-fructofuranose-1,2':2,3'-dianhydride + H2O
-
 r

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.178 Paenarthrobacter ureafaciens
-
-
-
4.2.1.178 Paenarthrobacter ureafaciens ATCC 21124
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.178 alpha-D-fructofuranose-beta-D-fructofuranose-1,2':2,3'-dianhydride + H2O
-
 Paenarthrobacter ureafaciens inulobiose
-
 r
4.2.1.178 alpha-D-fructofuranose-beta-D-fructofuranose-1,2':2,3'-dianhydride + H2O
-
 Paenarthrobacter ureafaciens ATCC 21124 inulobiose
-
 r
4.2.1.178 inulobiose
-
 Paenarthrobacter ureafaciens alpha-D-fructofuranose-beta-D-fructofuranose-1,2':2,3'-dianhydride + H2O
-
 r
4.2.1.178 inulobiose
-
 Paenarthrobacter ureafaciens ATCC 21124 alpha-D-fructofuranose-beta-D-fructofuranose-1,2':2,3'-dianhydride + H2O
-
 r

Synonyms

EC Number Synonyms Comment Organism
4.2.1.178 DFA-IIIase
-
 Paenarthrobacter ureafaciens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.178 60
-
-
 Paenarthrobacter ureafaciens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.1.178 30 60 the enzyme shows about 70% activity after 10 h at 30°C. The enzyme shows about 70% activity after 6 h at 40°C. The enzyme shows about 50% activity after 6 h at 50°C. The enzyme shows about 50% activity after 3 h at 60°C. The enzyme is inactive after 24 h incubation at 30-60°C Paenarthrobacter ureafaciens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.178 6.5
-
-
 Paenarthrobacter ureafaciens

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.2.1.178 5 9 the enzyme shows high stability in the range of pH 5.0-9.0 retaining more than 80% activity after 24 h incubation Paenarthrobacter ureafaciens