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Literature summary extracted from

  • Hui, X.; Hua, S.H.; Wu, Q.Q.; Li, H.; Gao, W.Y.
    Antimicrobial mechanism of epigallocatechin gallate and gallocatechin gallate they target 1-deoxy-D-xylulose 5-phosphate reductoisomerase, the key enzyme of the MEP terpenoid biosynthetic pathway (2017), Arch. Biochem. Biophys., 622, 1-8 .
    View publication on PubMed

Application

EC Number Application Comment Organism
1.1.1.267 drug development enzyme DXR is a validated antimicrobial target Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.267 baicalein
-
 Escherichia coli
1.1.1.267 epigallocatechin gallate specifically inhibits the enzyme and has antimicrobial activity, competitive inhibition versus DXP and uncompetitive inhibition versus NADPH Escherichia coli
1.1.1.267 gallocatechin gallate specifically inhibits the enzyme and has antimicrobial activity, competitive inhibition versus DXP and uncompetitive inhibition versus NADPH Escherichia coli
1.1.1.267 additional information possible interactions between DXR and the catechine inhibitors are simulated via molecular docking simulation, detailed overview. Triton X-100 does not affect the inhibition of the enzyme by epigallocatechin gallate and gallocatechin gallate, but that by baicalein (positive control) Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.267 Mg2+ required Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.267 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ Escherichia coli
-
 2-C-methyl-D-erythritol 4-phosphate + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.267 Escherichia coli P45568
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.267 1-deoxy-D-xylulose 5-phosphate + NADPH + H+
-
 Escherichia coli 2-C-methyl-D-erythritol 4-phosphate + NADP+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.267 1-deoxy-D-xylulose 5-phosphate reductoisomerase
-
 Escherichia coli
1.1.1.267 DXR
-
 Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.267 37
-
 assay at Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.267 7
-
 assay at Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.267 NADP+
-
 Escherichia coli
1.1.1.267 NADPH
-
 Escherichia coli

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.1.267 0.0234
-
 gallocatechin gallate pH 7.0, 37°C, recombinant enzyme, uncompetitive versus NADPH Escherichia coli
1.1.1.267 0.0252
-
 gallocatechin gallate pH 7.0, 37°C, recombinant enzyme, competitive versus 1-deoxy-D-xylulose 5-phosphate Escherichia coli
1.1.1.267 0.1483
-
 epigallocatechin gallate pH 7.0, 37°C, recombinant enzyme, competitive versus 1-deoxy-D-xylulose 5-phosphate Escherichia coli
1.1.1.267 0.1794
-
 epigallocatechin gallate pH 7.0, 37°C, recombinant enzyme, uncompetitive versus NADPH Escherichia coli

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.1.1.267 0.0275
-
 pH 7.0, 37°C, recombinant enzyme Escherichia coli gallocatechin gallate
1.1.1.267 0.2098
-
 pH 7.0, 37°C, recombinant enzyme Escherichia coli epigallocatechin gallate

General Information

EC Number General Information Comment Organism
1.1.1.267 metabolism 1-deoxy-D-xylulose 5-phosphate reductoisomerase (DXR) is the first key enzyme in the MEP pathway for terpenoid biosynthesis Escherichia coli