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Literature summary extracted from

  • Schiessl, J.; Kosciow, K.; Garschagen, L.S.; Hoffmann, J.J.; Heymuth, J.; Franke, T.; Deppenmeier, U.
    Degradation of the low-calorie sugar substitute 5-ketofructose by different bacteria (2021), Appl. Microbiol. Biotechnol., 105, 2441-2453 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.123 heterologously overproduced in Escherichia coli Clostridium pasteurianum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.123 1.3
-
 5-dehydro-D-fructose pH and temperature not specified in the publication Clostridium pasteurianum
1.1.1.123 12.4
-
 NADPH pH and temperature not specified in the publication Clostridium pasteurianum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.123 35900
-
 molecular mass including Strep-tag Clostridium pasteurianum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.123 L-sorbose + NADP+ Clostridium pasteurianum
-
 5-dehydro-D-fructose + NADPH + H+
-
 ?
1.1.1.123 L-sorbose + NADP+ Clostridium pasteurianum DSM 525
-
 5-dehydro-D-fructose + NADPH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.123 Clostridium pasteurianum AJA48273.1
-
-
1.1.1.123 Clostridium pasteurianum DSM 525 AJA48273.1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.123
-
 Clostridium pasteurianum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.123 48.9
-
 pH and temperature not specified in the publication Clostridium pasteurianum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.123 1-phenyl-1,2-propandione + NADP+ 14.8% of the specific activity as compared to 5-dehydro-D-fructose Clostridium pasteurianum ?
-
 ?
1.1.1.123 1-phenyl-1,2-propandione + NADP+ 14.8% of the specific activity as compared to 5-dehydro-D-fructose Clostridium pasteurianum DSM 525 ?
-
 ?
1.1.1.123 5-dehydro-D-fructose + NADPH + H+
-
 Clostridium pasteurianum L-sorbose + NADP+
-
 ?
1.1.1.123 5-dehydro-D-fructose + NADPH + H+
-
 Clostridium pasteurianum DSM 525 L-sorbose + NADP+
-
 ?
1.1.1.123 diacetyl + NADP+ 38% of the specific activity as compared to 5-dehydro-D-fructose Clostridium pasteurianum ?
-
 ?
1.1.1.123 diacetyl + NADP+ 38% of the specific activity as compared to 5-dehydro-D-fructose Clostridium pasteurianum DSM 525 ?
-
 ?
1.1.1.123 L-sorbose + NADP+
-
 Clostridium pasteurianum 5-dehydro-D-fructose + NADPH + H+
-
 ?
1.1.1.123 L-sorbose + NADP+
-
 Clostridium pasteurianum DSM 525 5-dehydro-D-fructose + NADPH + H+
-
 ?
1.1.1.123 pyruvaldehyde + NADP+ 5% of the specific activity as compared to 5-dehydro-D-fructose Clostridium pasteurianum ?
-
 ?
1.1.1.123 pyruvaldehyde + NADP+ 5% of the specific activity as compared to 5-dehydro-D-fructose Clostridium pasteurianum DSM 525 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.123 homotetramer 4 * 35000, SDS-PAGE Clostridium pasteurianum

Synonyms

EC Number Synonyms Comment Organism
1.1.1.123 AJA48273.1
-
 Clostridium pasteurianum
1.1.1.123 CP-AKR
-
 Clostridium pasteurianum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.123 40
-
-
 Clostridium pasteurianum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.123 29.3
-
 5-dehydro-D-fructose pH and temperature not specified in the publication Clostridium pasteurianum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.123 6
-
-
 Clostridium pasteurianum

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.123 NADPH
-
 Clostridium pasteurianum

General Information

EC Number General Information Comment Organism
1.1.1.123 metabolism an important function in the degradation of this substrate and might be responsible for the formation of fructose, which is then channelled into the central metabolism of Clostridium pasteurianum Clostridium pasteurianum