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Literature summary extracted from

  • Jankowski, N.; Koschorreck, K.; Urlacher, V.B.
    High-level expression of aryl-alcohol oxidase 2 from Pleurotus eryngii in Pichia pastoris for production of fragrances and bioactive precursors (2020), Appl. Microbiol. Biotechnol., 104, 9205-9218 .
    View publication on PubMed

Application

EC Number Application Comment Organism
1.1.3.7 synthesis expression and secretion of AAO with yield of 315 mg/l using Pichia pastoris Pleurotus eryngii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.3.7 100000
-
PAGE Pleurotus eryngii

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.7 Pleurotus eryngii D3YBH4
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.1.3.7 glycoprotein about 60% glycosylation Pleurotus eryngii

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.3.7 recombinant protein Pleurotus eryngii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.7 (2E)-hept-2-en-1-ol + O2 31.9% of the activity with benzyl alcohol Pleurotus eryngii (2E)-hept-2-enal + H2O2
-
?
1.1.3.7 (2E)-hex-2-en-1-ol + O2 63.9% of the activity with benzyl alcohol Pleurotus eryngii (2E)-hex-2-enal + H2O2
-
?
1.1.3.7 (2E,4E)-hepta-2,4-dien-1-ol + O2 737% of the activity with benzyl alcohol Pleurotus eryngii (2E,4E)-hepta-2,4-dienal + H2O2
-
?
1.1.3.7 (2E,4E)-hexa-2,4-dien-1-ol + O2 807% of the activity with benzyl alcohol Pleurotus eryngii (2E,4E)-hexa-2,4-dienal + H2O2
-
?
1.1.3.7 (2H-1,3-benzodioxol-5-yl)methanol + O2 301% of the activity with benzyl alcohol Pleurotus eryngii 2H-1,3-benzodioxole-5-carbaldehyde + H2O2
-
?
1.1.3.7 (naphthalen-2-yl)methanol + O2 874% of the activity with benzyl alcohol Pleurotus eryngii naphthalene-2-carbaldehyde + H2O2
-
?
1.1.3.7 (pyrene-1-yl)methanol + O2 35% of the activity with benzyl alcohol Pleurotus eryngii pyrene-1-carbaldehyde + H2O2
-
?
1.1.3.7 (thiophen-2-yl)methanol + O2 15.8% of the activity with benzyl alcohol Pleurotus eryngii thiophene-2-carbaldehyde + H2O2
-
?
1.1.3.7 4-aminobenzyl alcohol + O2 18.6% of the activity with benzyl alcohol Pleurotus eryngii 4-aminobenzaldehyde + H2O2
-
?
1.1.3.7 anisyl alcohol + O2 647% of the activity with benzyl alcohol Pleurotus eryngii anisyl aldehyde + H2O2
-
?
1.1.3.7 benzyl alcohol + O2
-
Pleurotus eryngii benzaldehyde + H2O2
-
?
1.1.3.7 cinnamyl alcohol + O2 442% of the activity with benzyl alcohol Pleurotus eryngii cinnamyl aldehyde + H2O2
-
?
1.1.3.7 cumic alcohol + O2 149% of the activity with benzyl alcohol Pleurotus eryngii cumic aldehyde + H2O2
-
?
1.1.3.7 isovanillyl alcohol + O2 246% of the activity with benzyl alcohol Pleurotus eryngii isovanillyl aldehyde + H2O2
-
?
1.1.3.7 veratryl alcohol + O2 322% of the activity with benzyl alcohol Pleurotus eryngii veratryl aldehyde + H2O2
-
?

Subunits

EC Number Subunits Comment Organism
1.1.3.7 monomer 1 * 100000, recombinant protein, 1 * 70000, PNGase F-treated protein, SDS-PAGE, 1 * 60800, calculated from sequence Pleurotus eryngii

Synonyms

EC Number Synonyms Comment Organism
1.1.3.7 AAO2
-
Pleurotus eryngii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.3.7 4 50 1 h, 90% residual activity Pleurotus eryngii
1.1.3.7 55
-
1 h, 70% residual activity Pleurotus eryngii
1.1.3.7 60
-
1 h, 10% residual activity Pleurotus eryngii

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.3.7 3 9 1 h, 90% residual activity Pleurotus eryngii