EC Number | Application | Comment | Organism |
---|---|---|---|
2.7.4.B7 | synthesis | the enzyme can used to replenish ATP in ATP-consuming biochemical reactions | Cupriavidus necator |
EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.4.B7 | expression in Escherichia coli JM109 | Cupriavidus necator |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.4.B7 | Ca2+ | divalent cation required for activity. 5 mM CaCl2 show 48% of the activity compared to 5 mM MnCl2 | Cupriavidus necator | |
2.7.4.B7 | Mg2+ | divalent cation required for activity. 5 mM MgCl2 show 84% of the activity compared to 5 mM MnCl2 | Cupriavidus necator | |
2.7.4.B7 | Mn2+ | divalent cation required for activity. Highest activity with 5 mM MnCl2 | Cupriavidus necator | |
2.7.4.B7 | Zn2+ | divalent cation required for activity. 5 mM ZnCl2 show 21% of the activity compared to 5 mM MnCl2 | Cupriavidus necator |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.4.B7 | Cupriavidus necator | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.4.B7 | - |
Cupriavidus necator |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.4.B7 | ADP + (phosphate)n+1 | about 80% compared to the activity with UDP. Additional formation of adenosine tetraphosphate | Cupriavidus necator | ATP + (phosphate)n | - |
r | |
2.7.4.B7 | ATP + (phosphate)n | 98% compared to the activity with CTP | Cupriavidus necator | ADP + (phosphate)n+1 | - |
r | |
2.7.4.B7 | CDP + (phosphate)n+1 | about 80% compared to the activity with UDP. Additional formation of cytidine tetraphosphate | Cupriavidus necator | CTP + (phosphate)n | - |
r | |
2.7.4.B7 | CTP + (phosphate)n | - |
Cupriavidus necator | CDP + (phosphate)n+1 | - |
r | |
2.7.4.B7 | dTDP + (phosphate)n+1 | about 80% compared to the activity with UDP. Additional formation of deoxythymidine tetraphosphate | Cupriavidus necator | dTTP + (phosphate)n | - |
r | |
2.7.4.B7 | dTTP + (phosphate)n | 98% compared to the activity with CTP | Cupriavidus necator | dTDP + (phosphate)n+1 | - |
r | |
2.7.4.B7 | GDP + (phosphate)n+1 | about 95% compared to the activity with UDP. Additional formation of guanidine tetraphosphate | Cupriavidus necator | GTP + (phosphate)n | - |
r | |
2.7.4.B7 | GTP + (phosphate)n | about 90% compared to the activity with CTP | Cupriavidus necator | GDP + (phosphate)n+1 | - |
r | |
2.7.4.B7 | additional information | thiamine pyrophosphate is not used as a substrate by PPK2c | Cupriavidus necator | ? | - |
- |
|
2.7.4.B7 | UDP + (phosphate)n+1 | additional formation of uridine tetraphosphate | Cupriavidus necator | UTP + (phosphate)n | - |
r | |
2.7.4.B7 | UTP + (phosphate)n | about 90% compared to the activity with CTP | Cupriavidus necator | UDP + (phosphate)n+1 | - |
r |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.4.B7 | PPK2c | - |
Cupriavidus necator |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.4.B7 | 28 | 40 | - |
Cupriavidus necator |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.4.B7 | 10 | 40 | 10°C: about 55% of maximal activity, 28°C-40°C: maximal activity, 45°C: less than 5% of maximal activcity | Cupriavidus necator |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.4.B7 | 40 | - |
rapid inactivation above | Cupriavidus necator |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.7.4.B7 | 7 | 9 | - |
Cupriavidus necator |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.7.4.B7 | 6.5 | 10.5 | pH 6.5: about 85% of maximal activity, pH 10.5: about 70% of maximal activity | Cupriavidus necator |