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Literature summary extracted from

  • Takahashi, S.; Osugi, K.; Shimekake, Y.; Shinbo, A.; Abe, K.; Kera, Y.
    Characterization and improvement of substrate-binding affinity of D-aspartate oxidase of the thermophilic fungus Thermomyces dupontii (2019), Appl. Microbiol. Biotechnol., 103, 4053-4064 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.3.1 expression in Escherichia coli Thermomyces dupontii

Protein Variants

EC Number Protein Variants Comment Organism
1.4.3.1 F248Y mutation markedly increases substrate-binding affinity Thermomyces dupontii

General Stability

EC Number General Stability Organism
1.4.3.1 enzyme stability decreases following a decrease in protein concentration, and externally added FAD cannot repress the destabilization Thermomyces dupontii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.3.1 0.71
-
 D-glutamate mutant F248Y, pH 7, 60°C Thermomyces dupontii
1.4.3.1 0.87
-
 D-Aspartate mutant F248Y, pH 7, 60°C Thermomyces dupontii
1.4.3.1 2.16
-
 D-glutamate wild-type, pH 7, 60°C Thermomyces dupontii
1.4.3.1 6.7
-
 N-Methyl-D-aspartate mutant F248Y, pH 7, 60°C Thermomyces dupontii
1.4.3.1 8.77
-
 D-Aspartate wild-type, pH 7, 60°C Thermomyces dupontii
1.4.3.1 25.7
-
 D-glutamine mutant F248Y, pH 7, 60°C Thermomyces dupontii
1.4.3.1 103
-
 N-Methyl-D-aspartate wild-type, pH 7, 60°C Thermomyces dupontii
1.4.3.1 108
-
 D-glutamine wild-type, pH 7, 60°C Thermomyces dupontii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.3.1 38300
-
 gel filtration Thermomyces dupontii

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.1 Thermomyces dupontii
-
-
-
1.4.3.1 Thermomyces dupontii NBRC 30541
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.3.1 46.3
-
 37°C, pH 7.5 Thermomyces dupontii
1.4.3.1 169
-
 60°C, pH 7.5 Thermomyces dupontii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.1 D-aspartate + H2O + O2
-
 Thermomyces dupontii oxaloacetate + H2O2 + NH3
-
 ?
1.4.3.1 D-aspartate + H2O + O2
-
 Thermomyces dupontii NBRC 30541 oxaloacetate + H2O2 + NH3
-
 ?
1.4.3.1 D-glutamate + H2O + O2
-
 Thermomyces dupontii 2-oxoglutarate + NH3 + H2O2
-
 ?
1.4.3.1 D-glutamate + H2O + O2
-
 Thermomyces dupontii NBRC 30541 2-oxoglutarate + NH3 + H2O2
-
 ?
1.4.3.1 D-glutamine + H2O + O2
-
 Thermomyces dupontii 2-oxoglutaric acid + H2O2 + NH3
-
 ?
1.4.3.1 D-glutamine + H2O + O2
-
 Thermomyces dupontii NBRC 30541 2-oxoglutaric acid + H2O2 + NH3
-
 ?
1.4.3.1 N-methyl-D-aspartate + H2O + O2
-
 Thermomyces dupontii oxaloacetate + CH3NH2 + H2O2
-
 ?
1.4.3.1 N-methyl-D-aspartate + H2O + O2
-
 Thermomyces dupontii NBRC 30541 oxaloacetate + CH3NH2 + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.4.3.1 monomer 1 * 45000, SDS-PAGE Thermomyces dupontii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.4.3.1 60
-
-
 Thermomyces dupontii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.3.1 51
-
 T50 value Thermomyces dupontii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.4.3.1 39.9
-
 D-glutamine mutant F248Y, pH 7, 60°C Thermomyces dupontii
1.4.3.1 50.9
-
 D-Aspartate mutant F248Y, pH 7, 60°C Thermomyces dupontii
1.4.3.1 58.1
-
 D-glutamate mutant F248Y, pH 7, 60°C Thermomyces dupontii
1.4.3.1 68.9
-
 N-Methyl-D-aspartate mutant F248Y, pH 7, 60°C Thermomyces dupontii
1.4.3.1 123
-
 D-Aspartate wild-type, pH 7, 60°C Thermomyces dupontii
1.4.3.1 144
-
 D-glutamine wild-type, pH 7, 60°C Thermomyces dupontii
1.4.3.1 155
-
 N-Methyl-D-aspartate wild-type, pH 7, 60°C Thermomyces dupontii
1.4.3.1 217
-
 D-glutamate wild-type, pH 7, 60°C Thermomyces dupontii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.3.1 8
-
-
 Thermomyces dupontii

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.4.3.1 5 10
-
 Thermomyces dupontii

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.3.1 FAD typical absorption spectrum associated with flavoproteins with maxima at 273, 375, and 455 nm Thermomyces dupontii

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.4.3.1 1.3
-
 D-glutamine wild-type, pH 7, 60°C Thermomyces dupontii
1.4.3.1 1.5
-
 N-Methyl-D-aspartate wild-type, pH 7, 60°C Thermomyces dupontii
1.4.3.1 10.2
-
 N-Methyl-D-aspartate mutant F248Y, pH 7, 60°C Thermomyces dupontii
1.4.3.1 14
-
 D-Aspartate wild-type, pH 7, 60°C Thermomyces dupontii
1.4.3.1 15.5
-
 D-glutamine mutant F248Y, pH 7, 60°C Thermomyces dupontii
1.4.3.1 58.6
-
 D-Aspartate mutant F248Y, pH 7, 60°C Thermomyces dupontii
1.4.3.1 81
-
 D-glutamate mutant F248Y, pH 7, 60°C Thermomyces dupontii
1.4.3.1 100
-
 D-glutamate wild-type, pH 7, 60°C Thermomyces dupontii