EC Number | Application | Comment | Organism |
---|---|---|---|
1.4.3.2 | synthesis | purified aldehyde-tagged 6His-hcLAAO4 is covalently bound to a hexylamine resin via the Calpha-formylglycine residue. The immobilized enzyme can be reused repeatedly to generate phenylpyruvate from L-phenylalanine with a total turnover number of 17600 and is stable for over 40 days at 25 °C | Hebeloma cylindrosporum |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.4.3.2 | expression of isoform LAAO4 without signal sequence as fusion protein with a 6His tag in Escherichia coli | Hebeloma cylindrosporum |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.4.3.2 | additional information | presence of an N-terminal aldehyde tag does not change the properties of the enzyme | Hebeloma cylindrosporum |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.4.3.2 | 0.26 | - |
L-leucine | acid-activited recombinant protein, pH 7, 30°C | Hebeloma cylindrosporum | |
1.4.3.2 | 0.26 | - |
L-phenylalanine | untreated recombinant protein, pH 7, 30°C | Hebeloma cylindrosporum | |
1.4.3.2 | 0.27 | - |
L-leucine | untreated recombinant protein, pH 7, 30°C | Hebeloma cylindrosporum | |
1.4.3.2 | 0.46 | - |
L-glutamine | untreated recombinant protein, pH 7, 30°C | Hebeloma cylindrosporum | |
1.4.3.2 | 0.48 | - |
L-phenylalanine | acid-activited recombinant protein, pH 7, 30°C | Hebeloma cylindrosporum | |
1.4.3.2 | 0.56 | - |
L-glutamine | acid-activited recombinant protein, pH 7, 30°C | Hebeloma cylindrosporum | |
1.4.3.2 | 0.67 | - |
L-leucine methyl ester | acid-activited recombinant protein, pH 7, 30°C | Hebeloma cylindrosporum | |
1.4.3.2 | 0.69 | - |
L-leucine methyl ester | untreated recombinant protein, pH 7, 30°C | Hebeloma cylindrosporum |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.4.3.2 | Hebeloma cylindrosporum | S4S6Z0 | - |
- |
EC Number | Renatured (Comment) | Organism |
---|---|---|
1.4.3.2 | recombinant purified LAAO4 is activated by exposure to acidic pH, the detergent sodium dodecyl sulfate, or freezing | Hebeloma cylindrosporum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.4.3.2 | L-alanine + H2O + O2 | - |
Hebeloma cylindrosporum | 2-oxopropanoate + NH3 + H2O2 | - |
? | |
1.4.3.2 | L-alanine ethyl ester + H2O + O2 | - |
Hebeloma cylindrosporum | ethyl 2-oxopropanoate + NH3 + H2O2 | - |
? | |
1.4.3.2 | L-arginine + H2O + O2 | - |
Hebeloma cylindrosporum | 2-oxo-5-guanidinopentanoate + NH3 + H2O2 | - |
? | |
1.4.3.2 | L-asparagine + H2O + O2 | - |
Hebeloma cylindrosporum | 2-oxo-3-phenylpropanoate + NH3 + H2O2 | - |
? | |
1.4.3.2 | L-canavanine + H2O + O2 | - |
Hebeloma cylindrosporum | 4-[[(diaminomethylidene)amino]oxy]-2-oxobutanoate + NH3 + H2O2 | - |
? | |
1.4.3.2 | L-glutamate + H2O + O2 | - |
Hebeloma cylindrosporum | 2-oxoglutarate + NH3 + H2O2 | - |
? | |
1.4.3.2 | L-glutamate dimethyl ester + H2O + O2 | - |
Hebeloma cylindrosporum | 2-oxoglutarate dimethyl ester + NH3 + H2O2 | - |
? | |
1.4.3.2 | L-glutamine + H2O + O2 | - |
Hebeloma cylindrosporum | 2-oxoglutarate + NH3 + H2O2 | - |
? | |
1.4.3.2 | L-histidine + H2O + O2 | - |
Hebeloma cylindrosporum | 3-(1H-imidazol-4-yl)-2-oxopropanoate + NH3 + H2O2 | - |
? | |
1.4.3.2 | L-isoleucine + H2O + O2 | - |
Hebeloma cylindrosporum | 3-methyl-2-oxo-pentanoate + NH3 + H2O2 | - |
? | |
1.4.3.2 | L-leucine + H2O + O2 | - |
Hebeloma cylindrosporum | 4-methyl-2-oxopentanoate + NH3 + H2O2 | - |
? | |
1.4.3.2 | L-leucine ethyl ester + H2O + O2 | - |
Hebeloma cylindrosporum | ethyl 4-methyl-2-oxopentanoate + NH3 + H2O2 | - |
? | |
1.4.3.2 | L-leucine methyl ester + H2O + O2 | - |
Hebeloma cylindrosporum | methyl 4-methyl-2-oxopentanoate + NH3 + H2O2 | - |
? | |
1.4.3.2 | L-lysine + H2O + O2 | - |
Hebeloma cylindrosporum | 6-amino-2-oxohexanoate + NH3 + H2O2 | - |
? | |
1.4.3.2 | L-methionine + H2O + O2 | - |
Hebeloma cylindrosporum | 4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2 | - |
? | |
1.4.3.2 | L-norleucine + H2O + O2 | - |
Hebeloma cylindrosporum | 2-oxohexanoate + NH3 + H2O2 | - |
? | |
1.4.3.2 | L-ornithine + H2O + O2 | - |
Hebeloma cylindrosporum | 5-amino-2-oxopentanoate + NH3 + H2O2 | - |
? | |
1.4.3.2 | L-phenylalanine + H2O + O2 | - |
Hebeloma cylindrosporum | 2-oxo-3-phenylpropanoate + NH3 + H2O2 | - |
? | |
1.4.3.2 | L-phenylalanine methyl ester + H2O + O2 | - |
Hebeloma cylindrosporum | methyl 2-oxo-3-phenylpropanoate + NH3 + H2O2 | - |
? | |
1.4.3.2 | L-tyrosine + H2O + O2 | - |
Hebeloma cylindrosporum | 3-(4-hydroxyphenyl)pyruvate + NH3 + H2O2 | - |
? | |
1.4.3.2 | L-tyrosine methyl ester + H2O + O2 | - |
Hebeloma cylindrosporum | methyl 3-(4-hydroxyphenyl)pyruvate + NH3 + H2O2 | - |
? | |
1.4.3.2 | additional information | enzyme converts 14 proteinogenic L-amino acids with L-glutamine, L-leucine, L-methionine, L-phenylalanine, L-tyrosine, and L-lysine being the best substrates. Methyl esters of these L-amino acids are also accepted, and ethyl esters are converted but with lower activity. No substrate: L-tert-leucine | Hebeloma cylindrosporum | ? | - |
- |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.4.3.2 | LAAO4 | - |
Hebeloma cylindrosporum |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.4.3.2 | 0.83 | - |
L-phenylalanine | untreated recombinant protein, pH 7, 30°C | Hebeloma cylindrosporum | |
1.4.3.2 | 2.32 | - |
L-leucine | untreated recombinant protein, pH 7, 30°C | Hebeloma cylindrosporum | |
1.4.3.2 | 2.56 | - |
L-glutamine | untreated recombinant protein, pH 7, 30°C | Hebeloma cylindrosporum | |
1.4.3.2 | 2.6 | - |
L-leucine methyl ester | untreated recombinant protein, pH 7, 30°C | Hebeloma cylindrosporum | |
1.4.3.2 | 21.9 | - |
L-phenylalanine | acid-activited recombinant protein, pH 7, 30°C | Hebeloma cylindrosporum | |
1.4.3.2 | 27.8 | - |
L-leucine methyl ester | acid-activited recombinant protein, pH 7, 30°C | Hebeloma cylindrosporum | |
1.4.3.2 | 38.8 | - |
L-glutamine | acid-activited recombinant protein, pH 7, 30°C | Hebeloma cylindrosporum | |
1.4.3.2 | 45.3 | - |
L-leucine | acid-activited recombinant protein, pH 7, 30°C | Hebeloma cylindrosporum |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.4.3.2 | FAD | - |
Hebeloma cylindrosporum |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.4.3.2 | 3.14 | - |
L-phenylalanine | untreated recombinant protein, pH 7, 30°C | Hebeloma cylindrosporum | |
1.4.3.2 | 3.77 | - |
L-leucine methyl ester | untreated recombinant protein, pH 7, 30°C | Hebeloma cylindrosporum | |
1.4.3.2 | 5.58 | - |
L-glutamine | untreated recombinant protein, pH 7, 30°C | Hebeloma cylindrosporum | |
1.4.3.2 | 8.48 | - |
L-leucine | untreated recombinant protein, pH 7, 30°C | Hebeloma cylindrosporum | |
1.4.3.2 | 41.5 | - |
L-leucine methyl ester | acid-activited recombinant protein, pH 7, 30°C | Hebeloma cylindrosporum | |
1.4.3.2 | 45.2 | - |
L-phenylalanine | acid-activited recombinant protein, pH 7, 30°C | Hebeloma cylindrosporum | |
1.4.3.2 | 69.4 | - |
L-glutamine | acid-activited recombinant protein, pH 7, 30°C | Hebeloma cylindrosporum | |
1.4.3.2 | 176.2 | - |
L-leucine | acid-activited recombinant protein, pH 7, 30°C | Hebeloma cylindrosporum |