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Literature summary extracted from
- Characterization of new recombinant 3-ketosteroid-DELTA1-dehydrogenases for the biotransformation of steroids (2017), Appl. Microbiol. Biotechnol., 101, 6049-6060 .
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 1.3.99.4 | synthesis | recombinant enzyme application potential of the former in the synthesis of prednisolone, method evaluation and optimization | Rhodococcus erythropolis |
| 1.3.99.4 | synthesis | recombinant enzyme application potential of the former in the synthesis of prednisolone, method evaluation and optimization | Mycolicibacterium smegmatis |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.3.99.4 | gene ksdD, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree, recombinant overexpression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Mycolicibacterium neoaurum |
| 1.3.99.4 | gene kstd, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree, recombinant overexpression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Rhodococcus erythropolis |
| 1.3.99.4 | gene kstd, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree, recombinant overexpression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Mycolicibacterium neoaurum |
| 1.3.99.4 | gene kstd, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree, recombinant overexpression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Mycolicibacterium smegmatis |
| 1.3.99.4 | gene kstd2, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree, recombinant overexpression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Mycolicibacterium smegmatis |
| 1.3.99.4 | gene kstd3, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree, recombinant overexpression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Mycolicibacterium smegmatis |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.3.99.4 | 0.0123 | - |
cortexolone | pH 8.0, 30°C | Rhodococcus erythropolis |  |
| 1.3.99.4 | 0.014 | - |
androst-4,9(11)-diene-3,17-dione | pH 8.0, 30°C | Rhodococcus erythropolis | |
| 1.3.99.4 | 0.0178 | - |
21-acetoxy-pregna-4,9(11),16-triene-3,20-dinone | pH 8.0, 30°C | Rhodococcus erythropolis | |
| 1.3.99.4 | 0.0182 | - |
17alpha-hydroxypregn-4-ene-3,20-dione | pH 8.0, 30°C | Rhodococcus erythropolis | |
| 1.3.99.4 | 0.0193 | - |
21-chloro-17-hydroxy-pregna-4,9(11)-diene-3,20-dione | pH 8.0, 30°C | Rhodococcus erythropolis | |
| 1.3.99.4 | 0.0202 | - |
progesterone | pH 8.0, 30°C | Rhodococcus erythropolis | |
| 1.3.99.4 | 0.0226 | - |
testosterone | pH 8.0, 30°C | Rhodococcus erythropolis | |
| 1.3.99.4 | 0.0232 | - |
11beta,17alpha,21-trihydroxypregn-4-ene-3,20-dione acetate | pH 8.0, 30°C | Rhodococcus erythropolis | |
| 1.3.99.4 | 0.0272 | - |
cortisone | pH 8.0, 30°C | Rhodococcus erythropolis | |
| 1.3.99.4 | 0.0369 | - |
9alpha-hydroxy-4-androstene-3,17-dione | pH 8.0, 25°C | Mycolicibacterium smegmatis | |
| 1.3.99.4 | 0.0373 | - |
21-acetoxy-pregna-4,9(11),16-triene-3,20-dinone | pH 8.0, 25°C | Mycolicibacterium smegmatis | |
| 1.3.99.4 | 0.0401 | - |
11beta,17alpha,21-trihydroxypregn-4-ene-3,20-dione | pH 8.0, 25°C | Mycolicibacterium smegmatis | |
| 1.3.99.4 | 0.0463 | - |
11beta,17alpha,21-trihydroxypregn-4-ene-3,20-dione | pH 8.0, 30°C | Rhodococcus erythropolis | |
| 1.3.99.4 | 0.0481 | - |
4-androstene-3,17-dione | pH 8.0, 30°C | Rhodococcus erythropolis | |
| 1.3.99.4 | 0.0509 | - |
9alpha-hydroxy-4-androstene-3,17-dione | pH 8.0, 30°C | Rhodococcus erythropolis | |
| 1.3.99.4 | 0.0667 | - |
progesterone | pH 8.0, 25°C | Mycolicibacterium smegmatis | |
| 1.3.99.4 | 0.0707 | - |
testosterone | pH 8.0, 25°C | Mycolicibacterium smegmatis | |
| 1.3.99.4 | 0.075 | - |
pregna-4,9(11),16-triene-3,20-dione | pH 8.0, 25°C | Mycolicibacterium smegmatis | |
| 1.3.99.4 | 0.0853 | - |
cortisone | pH 8.0, 25°C | Mycolicibacterium smegmatis | |
| 1.3.99.4 | 0.0917 | - |
17alpha-hydroxypregn-4-ene-3,20-dione | pH 8.0, 25°C | Mycolicibacterium smegmatis | |
| 1.3.99.4 | 0.0933 | - |
cortexolone | pH 8.0, 25°C | Mycolicibacterium smegmatis | |
| 1.3.99.4 | 0.0985 | - |
21-chloro-17-hydroxy-pregna-4,9(11)-diene-3,20-dione | pH 8.0, 25°C | Mycolicibacterium smegmatis | |
| 1.3.99.4 | 0.1003 | - |
11beta,17alpha,21-trihydroxypregn-4-ene-3,20-dione acetate | pH 8.0, 25°C | Mycolicibacterium smegmatis | |
| 1.3.99.4 | 0.1385 | - |
4-androstene-3,17-dione | pH 8.0, 25°C | Mycolicibacterium smegmatis | |
| 1.3.99.4 | 0.2102 | - |
4-androsten-3-one-5-ene-17-carboxylic acid | pH 8.0, 25°C | Mycolicibacterium smegmatis | |
| 1.3.99.4 | 0.2121 | - |
androst-4,9(11)-diene-3,17-dione | pH 8.0, 25°C | Mycolicibacterium smegmatis | |
| 1.3.99.4 | 0.2462 | - |
4-androsten-3-one-5-ene-17-carboxylic acid | pH 8.0, 30°C | Rhodococcus erythropolis |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.3.99.4 | a 3-oxosteroid + acceptor | Rhodococcus erythropolis | - |
a 3-oxo-delta1-steroid + reduced acceptor | - |
? | |
| 1.3.99.4 | a 3-oxosteroid + acceptor | Mycolicibacterium neoaurum | - |
a 3-oxo-delta1-steroid + reduced acceptor | - |
? | |
| 1.3.99.4 | a 3-oxosteroid + acceptor | Mycolicibacterium smegmatis | - |
a 3-oxo-delta1-steroid + reduced acceptor | - |
? | |
| 1.3.99.4 | a 3-oxosteroid + acceptor | Rhodococcus erythropolis WY 1406 | - |
a 3-oxo-delta1-steroid + reduced acceptor | - |
? | |
| 1.3.99.4 | a 3-oxosteroid + acceptor | Rhodococcus erythropolis CGMCC 5096 | - |
a 3-oxo-delta1-steroid + reduced acceptor | - |
? | |
| 1.3.99.4 | a 3-oxosteroid + acceptor | Mycolicibacterium neoaurum 2967 | - |
a 3-oxo-delta1-steroid + reduced acceptor | - |
? | |
| 1.3.99.4 | a 3-oxosteroid + acceptor | Mycolicibacterium neoaurum 2966 | - |
a 3-oxo-delta1-steroid + reduced acceptor | - |
? | |
| 1.3.99.4 | a 3-oxosteroid + acceptor | Mycolicibacterium smegmatis ATCC 700084 | - |
a 3-oxo-delta1-steroid + reduced acceptor | - |
? | |
| 1.3.99.4 | a 3-oxosteroid + acceptor | Mycolicibacterium smegmatis mc2155 | - |
a 3-oxo-delta1-steroid + reduced acceptor | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.3.99.4 | Mycolicibacterium neoaurum | - |
- |
- |
| 1.3.99.4 | Mycolicibacterium neoaurum | C6KID9 | - |
- |
| 1.3.99.4 | Mycolicibacterium neoaurum | C8CJG6 | - |
- |
| 1.3.99.4 | Mycolicibacterium neoaurum 2966 | - |
- |
- |
| 1.3.99.4 | Mycolicibacterium neoaurum 2966 | C6KID9 | - |
- |
| 1.3.99.4 | Mycolicibacterium neoaurum 2966 | C8CJG6 | - |
- |
| 1.3.99.4 | Mycolicibacterium neoaurum 2967 | C6KID9 | - |
- |
| 1.3.99.4 | Mycolicibacterium smegmatis | A0QW99 | - |
- |
| 1.3.99.4 | Mycolicibacterium smegmatis | A0R1T2 | - |
- |
| 1.3.99.4 | Mycolicibacterium smegmatis | A0R1T7 | - |
- |
| 1.3.99.4 | Mycolicibacterium smegmatis ATCC 700084 | A0QW99 | - |
- |
| 1.3.99.4 | Mycolicibacterium smegmatis ATCC 700084 | A0R1T2 | - |
- |
| 1.3.99.4 | Mycolicibacterium smegmatis ATCC 700084 | A0R1T7 | - |
- |
| 1.3.99.4 | Mycolicibacterium smegmatis mc2155 | A0QW99 | - |
- |
| 1.3.99.4 | Mycolicibacterium smegmatis mc2155 | A0R1T2 | - |
- |
| 1.3.99.4 | Mycolicibacterium smegmatis mc2155 | A0R1T7 | - |
- |
| 1.3.99.4 | Rhodococcus erythropolis | Q9RA02 | - |
- |
| 1.3.99.4 | Rhodococcus erythropolis CGMCC 5096 | Q9RA02 | - |
- |
| 1.3.99.4 | Rhodococcus erythropolis WY 1406 | Q9RA02 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.3.99.4 | recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Rhodococcus erythropolis |
| 1.3.99.4 | recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Mycolicibacterium smegmatis |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.3.99.4 | 4°C, purified recombinant enzyme, stable in 50 mM Tris-HCl, pH 8.0, for at least 2 weeks | Rhodococcus erythropolis |
| 1.3.99.4 | 4°C, purified recombinant enzyme, stable in 50 mM Tris-HCl, pH 8.0, for at least 2 weeks | Mycolicibacterium smegmatis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.3.99.4 | 11beta,17alpha,21-trihydroxypregn-4-ene-3,20-dione + 2,6-dichlorphenolindophenol | hydrocortisone | Rhodococcus erythropolis | 1,4-pregnene-17alpha,21-diol-3,11,20-trione + reduced 2,6-dichlorphenolindophenol | - |
? | |
| 1.3.99.4 | 11beta,17alpha,21-trihydroxypregn-4-ene-3,20-dione + 2,6-dichlorphenolindophenol | hydrocortisone | Mycolicibacterium smegmatis | 1,4-pregnene-17alpha,21-diol-3,11,20-trione + reduced 2,6-dichlorphenolindophenol | - |
? | |
| 1.3.99.4 | 11beta,17alpha,21-trihydroxypregn-4-ene-3,20-dione + acceptor | hydrocortisone | Rhodococcus erythropolis | 1,4-pregnene-17alpha,21-diol-3,11,20-trione + reduced acceptor | - |
? | |
| 1.3.99.4 | 11beta,17alpha,21-trihydroxypregn-4-ene-3,20-dione + acceptor | hydrocortisone | Mycolicibacterium smegmatis | 1,4-pregnene-17alpha,21-diol-3,11,20-trione + reduced acceptor | - |
? | |
| 1.3.99.4 | 11beta,17alpha,21-trihydroxypregn-4-ene-3,20-dione + phenazine methosulfate | hydrocortisone | Rhodococcus erythropolis | 1,4-pregnene-17alpha,21-diol-3,11,20-trione + reduced phenazine methosulfate | - |
? | |
| 1.3.99.4 | 11beta,17alpha,21-trihydroxypregn-4-ene-3,20-dione + phenazine methosulfate | hydrocortisone | Mycolicibacterium smegmatis | 1,4-pregnene-17alpha,21-diol-3,11,20-trione + reduced phenazine methosulfate | - |
? | |
| 1.3.99.4 | 11beta,17alpha,21-trihydroxypregn-4-ene-3,20-dione acetate + acceptor | hydrocortisone | Rhodococcus erythropolis | 1,4-pregnene-17alpha,21-diol-3,11,20-trione acetate + reduced acceptor | - |
? | |
| 1.3.99.4 | 11beta,17alpha,21-trihydroxypregn-4-ene-3,20-dione acetate + acceptor | hydrocortisone | Mycolicibacterium smegmatis | 1,4-pregnene-17alpha,21-diol-3,11,20-trione acetate + reduced acceptor | - |
? | |
| 1.3.99.4 | 11beta,17alpha,21-trihydroxypregn-4-ene-3,20-dione acetate + acceptor | hydrocortisone acetate | Rhodococcus erythropolis | 1,4-pregnene-17alpha,21-diol-3,11,20-trione acetate + reduced acceptor | - |
? | |
| 1.3.99.4 | 11beta,17alpha,21-trihydroxypregn-4-ene-3,20-dione acetate + acceptor | hydrocortisone acetate | Mycolicibacterium smegmatis | 1,4-pregnene-17alpha,21-diol-3,11,20-trione acetate + reduced acceptor | - |
? | |
| 1.3.99.4 | 17alpha-hydroxypregn-4-ene-3,20-dione + acceptor | - |
Rhodococcus erythropolis | ? + reduced acceptor | - |
? | |
| 1.3.99.4 | 17alpha-hydroxypregn-4-ene-3,20-dione + acceptor | - |
Mycolicibacterium smegmatis | ? + reduced acceptor | - |
? | |
| 1.3.99.4 | 21-acetoxy-pregna-4,9(11),16-triene-3,20-dinone + acceptor | - |
Rhodococcus erythropolis | ? + reduced acceptor | - |
? | |
| 1.3.99.4 | 21-acetoxy-pregna-4,9(11),16-triene-3,20-dinone + acceptor | - |
Mycolicibacterium smegmatis | ? + reduced acceptor | - |
? | |
| 1.3.99.4 | 21-chloro-17-hydroxy-pregna-4,9(11)-diene-3,20-dione + acceptor | - |
Rhodococcus erythropolis | ? + reduced acceptor | - |
? | |
| 1.3.99.4 | 21-chloro-17-hydroxy-pregna-4,9(11)-diene-3,20-dione + acceptor | - |
Mycolicibacterium smegmatis | ? + reduced acceptor | - |
? | |
| 1.3.99.4 | 4-androsten-3-one-5-ene-17-carboxylic acid + acceptor | - |
Rhodococcus erythropolis | ? + reduced acceptor | - |
? | |
| 1.3.99.4 | 4-androsten-3-one-5-ene-17-carboxylic acid + acceptor | - |
Mycolicibacterium smegmatis | ? + reduced acceptor | - |
? | |
| 1.3.99.4 | 4-androstene-3,17-dione + acceptor | - |
Rhodococcus erythropolis | 1,4-androstadiene-3,17-dione + reduced acceptor | - |
? | |
| 1.3.99.4 | 4-androstene-3,17-dione + acceptor | - |
Mycolicibacterium smegmatis | 1,4-androstadiene-3,17-dione + reduced acceptor | - |
? | |
| 1.3.99.4 | 4-androstene-3,17-dione + acceptor | - |
Rhodococcus erythropolis WY 1406 | 1,4-androstadiene-3,17-dione + reduced acceptor | - |
? | |
| 1.3.99.4 | 4-androstene-3,17-dione + acceptor | - |
Rhodococcus erythropolis CGMCC 5096 | 1,4-androstadiene-3,17-dione + reduced acceptor | - |
? | |
| 1.3.99.4 | 4-androstene-3,17-dione + acceptor | - |
Mycolicibacterium smegmatis ATCC 700084 | 1,4-androstadiene-3,17-dione + reduced acceptor | - |
? | |
| 1.3.99.4 | 4-androstene-3,17-dione + acceptor | - |
Mycolicibacterium smegmatis mc2155 | 1,4-androstadiene-3,17-dione + reduced acceptor | - |
? | |
| 1.3.99.4 | 9alpha-hydroxy-4-androstene-3,17-dione + acceptor | - |
Rhodococcus erythropolis | 9alpha-hydroxy-1,4-androstadiene 3,17-dione + reduced acceptor | - |
? | |
| 1.3.99.4 | 9alpha-hydroxy-4-androstene-3,17-dione + acceptor | - |
Mycolicibacterium smegmatis | 9alpha-hydroxy-1,4-androstadiene 3,17-dione + reduced acceptor | - |
? | |
| 1.3.99.4 | 9alpha-hydroxy-4-androstene-3,17-dione + acceptor | - |
Rhodococcus erythropolis WY 1406 | 9alpha-hydroxy-1,4-androstadiene 3,17-dione + reduced acceptor | - |
? | |
| 1.3.99.4 | 9alpha-hydroxy-4-androstene-3,17-dione + acceptor | - |
Rhodococcus erythropolis CGMCC 5096 | 9alpha-hydroxy-1,4-androstadiene 3,17-dione + reduced acceptor | - |
? | |
| 1.3.99.4 | 9alpha-hydroxy-4-androstene-3,17-dione + acceptor | - |
Mycolicibacterium smegmatis ATCC 700084 | 9alpha-hydroxy-1,4-androstadiene 3,17-dione + reduced acceptor | - |
? | |
| 1.3.99.4 | 9alpha-hydroxy-4-androstene-3,17-dione + acceptor | - |
Mycolicibacterium smegmatis mc2155 | 9alpha-hydroxy-1,4-androstadiene 3,17-dione + reduced acceptor | - |
? | |
| 1.3.99.4 | a 3-oxosteroid + acceptor | - |
Rhodococcus erythropolis | a 3-oxo-delta1-steroid + reduced acceptor | - |
? | |
| 1.3.99.4 | a 3-oxosteroid + acceptor | - |
Mycolicibacterium neoaurum | a 3-oxo-delta1-steroid + reduced acceptor | - |
? | |
| 1.3.99.4 | a 3-oxosteroid + acceptor | - |
Mycolicibacterium smegmatis | a 3-oxo-delta1-steroid + reduced acceptor | - |
? | |
| 1.3.99.4 | a 3-oxosteroid + acceptor | - |
Rhodococcus erythropolis WY 1406 | a 3-oxo-delta1-steroid + reduced acceptor | - |
? | |
| 1.3.99.4 | a 3-oxosteroid + acceptor | - |
Rhodococcus erythropolis CGMCC 5096 | a 3-oxo-delta1-steroid + reduced acceptor | - |
? | |
| 1.3.99.4 | a 3-oxosteroid + acceptor | - |
Mycolicibacterium neoaurum 2967 | a 3-oxo-delta1-steroid + reduced acceptor | - |
? | |
| 1.3.99.4 | a 3-oxosteroid + acceptor | - |
Mycolicibacterium neoaurum 2966 | a 3-oxo-delta1-steroid + reduced acceptor | - |
? | |
| 1.3.99.4 | a 3-oxosteroid + acceptor | - |
Mycolicibacterium smegmatis ATCC 700084 | a 3-oxo-delta1-steroid + reduced acceptor | - |
? | |
| 1.3.99.4 | a 3-oxosteroid + acceptor | - |
Mycolicibacterium smegmatis mc2155 | a 3-oxo-delta1-steroid + reduced acceptor | - |
? | |
| 1.3.99.4 | androst-4,9(11)-diene-3,17-dione + acceptor | - |
Rhodococcus erythropolis | ? + reduced acceptor | - |
? | |
| 1.3.99.4 | androst-4,9(11)-diene-3,17-dione + acceptor | - |
Mycolicibacterium smegmatis | ? + reduced acceptor | - |
? | |
| 1.3.99.4 | cortexolone + 2,6-dichlorophenol-indophenol | - |
Rhodococcus erythropolis | ? + reduced 2,6-dichlorophenol-indophenol | - |
? | |
| 1.3.99.4 | cortexolone + 2,6-dichlorophenol-indophenol | - |
Mycolicibacterium smegmatis | ? + reduced 2,6-dichlorophenol-indophenol | - |
? | |
| 1.3.99.4 | cortisone + acceptor | - |
Rhodococcus erythropolis | ? + reduced acceptor | - |
? | |
| 1.3.99.4 | cortisone + acceptor | - |
Mycolicibacterium smegmatis | ? + reduced acceptor | - |
? | |
| 1.3.99.4 | additional information | KstD from Rhodococcus erythropolis WY 1406 exhibits high catalytic efficiency toward androst-4,9(11)-diene-3,17-dione, 21-acetoxy-pregna-4,9(11),16-triene-3,20-dione, and cortexolone, where in all three cases the Km values are 2.5-4fold lower than that toward hydrocortisone. 4-Androstene-3,17-dione is a good substrate. Substrate specificity, overview. Assay method evaluation and optimization | Rhodococcus erythropolis | ? | - |
- |
|
| 1.3.99.4 | additional information | the purified KstD from Mycobacterium smegmatis mc2155 displays high catalytic efficiency toward hydrocortisone, progesterone, and 9alpha-hydroxy-4-androstene-3,17-dione (9-OH-AD), and has highest affinity toward 9-OH-AD. 4-Androstene-3,17-dione is a good substrate. Substrate specificity, overview. Assay method evaluation and optimization. No or poor activity with 11beta,17-dihydroxy-6alpha-methyl-pregn-4-ene-3,20-dione and anecortave | Mycolicibacterium smegmatis | ? | - |
- |
|
| 1.3.99.4 | additional information | KstD from Rhodococcus erythropolis WY 1406 exhibits high catalytic efficiency toward androst-4,9(11)-diene-3,17-dione, 21-acetoxy-pregna-4,9(11),16-triene-3,20-dione, and cortexolone, where in all three cases the Km values are 2.5-4fold lower than that toward hydrocortisone. 4-Androstene-3,17-dione is a good substrate. Substrate specificity, overview. Assay method evaluation and optimization | Rhodococcus erythropolis WY 1406 | ? | - |
- |
|
| 1.3.99.4 | additional information | KstD from Rhodococcus erythropolis WY 1406 exhibits high catalytic efficiency toward androst-4,9(11)-diene-3,17-dione, 21-acetoxy-pregna-4,9(11),16-triene-3,20-dione, and cortexolone, where in all three cases the Km values are 2.5-4fold lower than that toward hydrocortisone. 4-Androstene-3,17-dione is a good substrate. Substrate specificity, overview. Assay method evaluation and optimization | Rhodococcus erythropolis CGMCC 5096 | ? | - |
- |
|
| 1.3.99.4 | additional information | the purified KstD from Mycobacterium smegmatis mc2155 displays high catalytic efficiency toward hydrocortisone, progesterone, and 9alpha-hydroxy-4-androstene-3,17-dione (9-OH-AD), and has highest affinity toward 9-OH-AD. 4-Androstene-3,17-dione is a good substrate. Substrate specificity, overview. Assay method evaluation and optimization. No or poor activity with 11beta,17-dihydroxy-6alpha-methyl-pregn-4-ene-3,20-dione and anecortave | Mycolicibacterium smegmatis ATCC 700084 | ? | - |
- |
|
| 1.3.99.4 | additional information | the purified KstD from Mycobacterium smegmatis mc2155 displays high catalytic efficiency toward hydrocortisone, progesterone, and 9alpha-hydroxy-4-androstene-3,17-dione (9-OH-AD), and has highest affinity toward 9-OH-AD. 4-Androstene-3,17-dione is a good substrate. Substrate specificity, overview. Assay method evaluation and optimization. No or poor activity with 11beta,17-dihydroxy-6alpha-methyl-pregn-4-ene-3,20-dione and anecortave | Mycolicibacterium smegmatis mc2155 | ? | - |
- |
|
| 1.3.99.4 | pregna-4,9(11),16-triene-3,20-dione + acceptor | - |
Mycolicibacterium smegmatis | ? + reduced acceptor | - |
? | |
| 1.3.99.4 | progesterone + acceptor | - |
Rhodococcus erythropolis | pregna-1,4-diene-3,20-dione + reduced acceptor | - |
? | |
| 1.3.99.4 | progesterone + acceptor | - |
Mycolicibacterium smegmatis | pregna-1,4-diene-3,20-dione + reduced acceptor | - |
? | |
| 1.3.99.4 | testosterone + 2,6-dichlorophenol-indophenol | - |
Rhodococcus erythropolis | 17beta-hydroxyandrost-1,4-diene-3-one + reduced 2,6-dichlorophenol-indophenol | - |
? | |
| 1.3.99.4 | testosterone + 2,6-dichlorophenol-indophenol | - |
Mycolicibacterium smegmatis | 17beta-hydroxyandrost-1,4-diene-3-one + reduced 2,6-dichlorophenol-indophenol | - |
? | |
| 1.3.99.4 | testosterone + 2,6-dichlorophenol-indophenol | - |
Rhodococcus erythropolis WY 1406 | 17beta-hydroxyandrost-1,4-diene-3-one + reduced 2,6-dichlorophenol-indophenol | - |
? | |
| 1.3.99.4 | testosterone + 2,6-dichlorophenol-indophenol | - |
Rhodococcus erythropolis CGMCC 5096 | 17beta-hydroxyandrost-1,4-diene-3-one + reduced 2,6-dichlorophenol-indophenol | - |
? | |
| 1.3.99.4 | testosterone + 2,6-dichlorophenol-indophenol | - |
Mycolicibacterium smegmatis ATCC 700084 | 17beta-hydroxyandrost-1,4-diene-3-one + reduced 2,6-dichlorophenol-indophenol | - |
? | |
| 1.3.99.4 | testosterone + 2,6-dichlorophenol-indophenol | - |
Mycolicibacterium smegmatis mc2155 | 17beta-hydroxyandrost-1,4-diene-3-one + reduced 2,6-dichlorophenol-indophenol | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.3.99.4 | ? | x * 53800, recombinant enzyme, SDS-PAGE, x * 53073, sequence calculation | Rhodococcus erythropolis |
| 1.3.99.4 | ? | x * 55200, recombinant enzyme, SDS-PAGE, x * 54806, sequence calculation | Mycolicibacterium smegmatis |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.3.99.4 | 3-ketosteroid-DELTA1-dehydrogenase | - |
Rhodococcus erythropolis |
| 1.3.99.4 | 3-ketosteroid-DELTA1-dehydrogenase | - |
Mycolicibacterium smegmatis |
| 1.3.99.4 | KsdD | - |
Mycolicibacterium neoaurum |
| 1.3.99.4 | KstD | - |
Rhodococcus erythropolis |
| 1.3.99.4 | KstD | - |
Mycolicibacterium smegmatis |
| 1.3.99.4 | MnKstD1 | - |
Mycolicibacterium neoaurum |
| 1.3.99.4 | MnKstD2 | - |
Mycolicibacterium neoaurum |
| 1.3.99.4 | MsKstD1 | - |
Mycolicibacterium smegmatis |
| 1.3.99.4 | MsKstD2 | - |
Mycolicibacterium smegmatis |
| 1.3.99.4 | MsKstD3 | - |
Mycolicibacterium smegmatis |
| 1.3.99.4 | MsKstD4 | - |
Mycolicibacterium neoaurum |
| 1.3.99.4 | ReKstD1 | - |
Rhodococcus erythropolis |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.3.99.4 | 25 | - |
recombinant enzyme | Mycolicibacterium smegmatis |
| 1.3.99.4 | 30 | - |
assay at | Mycolicibacterium neoaurum |
| 1.3.99.4 | 30 | - |
assay at | Mycolicibacterium smegmatis |
| 1.3.99.4 | 30 | - |
recombinant enzyme | Rhodococcus erythropolis |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.3.99.4 | 10.2 | - |
androst-4,9(11)-diene-3,17-dione | pH 8.0, 25°C | Mycolicibacterium smegmatis | |
| 1.3.99.4 | 12.8 | - |
21-chloro-17-hydroxy-pregna-4,9(11)-diene-3,20-dione | pH 8.0, 30°C | Rhodococcus erythropolis | |
| 1.3.99.4 | 13.7 | - |
21-acetoxy-pregna-4,9(11),16-triene-3,20-dinone | pH 8.0, 25°C | Mycolicibacterium smegmatis | |
| 1.3.99.4 | 14.4 | - |
pregna-4,9(11),16-triene-3,20-dione | pH 8.0, 25°C | Mycolicibacterium smegmatis | |
| 1.3.99.4 | 20.3 | - |
11beta,17alpha,21-trihydroxypregn-4-ene-3,20-dione acetate | pH 8.0, 30°C | Rhodococcus erythropolis | |
| 1.3.99.4 | 20.6 | - |
cortexolone | pH 8.0, 30°C | Rhodococcus erythropolis | |
| 1.3.99.4 | 21.4 | - |
9alpha-hydroxy-4-androstene-3,17-dione | pH 8.0, 30°C | Rhodococcus erythropolis | |
| 1.3.99.4 | 21.4 | - |
17alpha-hydroxypregn-4-ene-3,20-dione | pH 8.0, 30°C | Rhodococcus erythropolis | |
| 1.3.99.4 | 22.3 | - |
androst-4,9(11)-diene-3,17-dione | pH 8.0, 30°C | Rhodococcus erythropolis | |
| 1.3.99.4 | 24.6 | - |
cortisone | pH 8.0, 30°C | Rhodococcus erythropolis | |
| 1.3.99.4 | 25.4 | - |
progesterone | pH 8.0, 30°C | Rhodococcus erythropolis | |
| 1.3.99.4 | 25.8 | - |
testosterone | pH 8.0, 30°C | Rhodococcus erythropolis | |
| 1.3.99.4 | 26.9 | - |
21-acetoxy-pregna-4,9(11),16-triene-3,20-dinone | pH 8.0, 30°C | Rhodococcus erythropolis | |
| 1.3.99.4 | 40.8 | - |
4-androsten-3-one-5-ene-17-carboxylic acid | pH 8.0, 30°C | Rhodococcus erythropolis | |
| 1.3.99.4 | 47.5 | - |
4-androstene-3,17-dione | pH 8.0, 30°C | Rhodococcus erythropolis | |
| 1.3.99.4 | 50.2 | - |
11beta,17alpha,21-trihydroxypregn-4-ene-3,20-dione | pH 8.0, 30°C | Rhodococcus erythropolis | |
| 1.3.99.4 | 51.5 | - |
4-androstene-3,17-dione | pH 8.0, 25°C | Mycolicibacterium smegmatis | |
| 1.3.99.4 | 52.2 | - |
cortisone | pH 8.0, 25°C | Mycolicibacterium smegmatis | |
| 1.3.99.4 | 58.7 | - |
9alpha-hydroxy-4-androstene-3,17-dione | pH 8.0, 25°C | Mycolicibacterium smegmatis | |
| 1.3.99.4 | 60.3 | - |
11beta,17alpha,21-trihydroxypregn-4-ene-3,20-dione | pH 8.0, 25°C | Mycolicibacterium smegmatis | |
| 1.3.99.4 | 63.5 | - |
21-chloro-17-hydroxy-pregna-4,9(11)-diene-3,20-dione | pH 8.0, 25°C | Mycolicibacterium smegmatis | |
| 1.3.99.4 | 77.2 | - |
11beta,17alpha,21-trihydroxypregn-4-ene-3,20-dione acetate | pH 8.0, 25°C | Mycolicibacterium smegmatis | |
| 1.3.99.4 | 77.5 | - |
testosterone | pH 8.0, 25°C | Mycolicibacterium smegmatis | |
| 1.3.99.4 | 94.1 | - |
4-androsten-3-one-5-ene-17-carboxylic acid | pH 8.0, 25°C | Mycolicibacterium smegmatis | |
| 1.3.99.4 | 94.2 | - |
cortexolone | pH 8.0, 25°C | Mycolicibacterium smegmatis | |
| 1.3.99.4 | 110.4 | - |
17alpha-hydroxypregn-4-ene-3,20-dione | pH 8.0, 25°C | Mycolicibacterium smegmatis | |
| 1.3.99.4 | 127.8 | - |
progesterone | pH 8.0, 25°C | Mycolicibacterium smegmatis | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.3.99.4 | 8 | - |
recombinant enzyme | Rhodococcus erythropolis |
| 1.3.99.4 | 8 | - |
recombinant enzyme | Mycolicibacterium smegmatis |
| 1.3.99.4 | 8 | - |
assay at | Mycolicibacterium neoaurum |
| 1.3.99.4 | 8 | - |
assay at | Mycolicibacterium smegmatis |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.3.99.4 | 2,6-dichlorophenolindophenol | - |
Rhodococcus erythropolis | |
| 1.3.99.4 | 2,6-dichlorophenolindophenol | - |
Mycolicibacterium smegmatis | |
| 1.3.99.4 | phenazine methosulfate | - |
Rhodococcus erythropolis | |
| 1.3.99.4 | phenazine methosulfate | preferred cofactor | Mycolicibacterium smegmatis | |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.3.99.4 | 6 | 12 | cortisone | pH 8.0, 25°C | Mycolicibacterium smegmatis | |
| 1.3.99.4 | 48.1 | - |
androst-4,9(11)-diene-3,17-dione | pH 8.0, 25°C | Mycolicibacterium smegmatis | |
| 1.3.99.4 | 165.7 | - |
4-androsten-3-one-5-ene-17-carboxylic acid | pH 8.0, 30°C | Rhodococcus erythropolis | |
| 1.3.99.4 | 192 | - |
pregna-4,9(11),16-triene-3,20-dione | pH 8.0, 25°C | Mycolicibacterium smegmatis | |
| 1.3.99.4 | 367.3 | - |
21-acetoxy-pregna-4,9(11),16-triene-3,20-dinone | pH 8.0, 25°C | Mycolicibacterium smegmatis | |
| 1.3.99.4 | 371.8 | - |
4-androstene-3,17-dione | pH 8.0, 25°C | Mycolicibacterium smegmatis | |
| 1.3.99.4 | 420.4 | - |
9alpha-hydroxy-4-androstene-3,17-dione | pH 8.0, 30°C | Rhodococcus erythropolis | |
| 1.3.99.4 | 447.7 | - |
4-androsten-3-one-5-ene-17-carboxylic acid | pH 8.0, 25°C | Mycolicibacterium smegmatis | |
| 1.3.99.4 | 644.7 | - |
21-chloro-17-hydroxy-pregna-4,9(11)-diene-3,20-dione | pH 8.0, 25°C | Mycolicibacterium smegmatis | |
| 1.3.99.4 | 663.2 | - |
21-chloro-17-hydroxy-pregna-4,9(11)-diene-3,20-dione | pH 8.0, 30°C | Rhodococcus erythropolis | |
| 1.3.99.4 | 769.7 | - |
11beta,17alpha,21-trihydroxypregn-4-ene-3,20-dione acetate | pH 8.0, 25°C | Mycolicibacterium smegmatis | |
| 1.3.99.4 | 875 | - |
11beta,17alpha,21-trihydroxypregn-4-ene-3,20-dione acetate | pH 8.0, 30°C | Rhodococcus erythropolis | |
| 1.3.99.4 | 904.4 | - |
cortisone | pH 8.0, 30°C | Rhodococcus erythropolis | |
| 1.3.99.4 | 987.5 | - |
4-androstene-3,17-dione | pH 8.0, 30°C | Rhodococcus erythropolis | |
| 1.3.99.4 | 1009.6 | - |
cortexolone | pH 8.0, 25°C | Mycolicibacterium smegmatis | |
| 1.3.99.4 | 1084.2 | - |
11beta,17alpha,21-trihydroxypregn-4-ene-3,20-dione | pH 8.0, 30°C | Rhodococcus erythropolis | |
| 1.3.99.4 | 1096.2 | - |
testosterone | pH 8.0, 25°C | Mycolicibacterium smegmatis | |
| 1.3.99.4 | 1141.6 | - |
testosterone | pH 8.0, 30°C | Rhodococcus erythropolis | |
| 1.3.99.4 | 1175.8 | - |
17alpha-hydroxypregn-4-ene-3,20-dione | pH 8.0, 30°C | Rhodococcus erythropolis | |
| 1.3.99.4 | 1203.9 | - |
17alpha-hydroxypregn-4-ene-3,20-dione | pH 8.0, 25°C | Mycolicibacterium smegmatis | |
| 1.3.99.4 | 1257.4 | - |
progesterone | pH 8.0, 30°C | Rhodococcus erythropolis | |
| 1.3.99.4 | 1503.7 | - |
11beta,17alpha,21-trihydroxypregn-4-ene-3,20-dione | pH 8.0, 25°C | Mycolicibacterium smegmatis | |
| 1.3.99.4 | 1511.2 | - |
21-acetoxy-pregna-4,9(11),16-triene-3,20-dinone | pH 8.0, 30°C | Rhodococcus erythropolis | |
| 1.3.99.4 | 1590.8 | - |
9alpha-hydroxy-4-androstene-3,17-dione | pH 8.0, 25°C | Mycolicibacterium smegmatis | |
| 1.3.99.4 | 1592.9 | - |
androst-4,9(11)-diene-3,17-dione | pH 8.0, 30°C | Rhodococcus erythropolis | |
| 1.3.99.4 | 1674.8 | - |
cortexolone | pH 8.0, 30°C | Rhodococcus erythropolis | |
| 1.3.99.4 | 1916 | - |
progesterone | pH 8.0, 25°C | Mycolicibacterium smegmatis | |
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