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Literature summary extracted from

  • Khan, R.; Zeb, A.; Roy, N.; Magar, R.; Kim, H.; Lee, K.; Lee, S.
    Biochemical and structural basis of triclosan resistance in a novel enoyl-acyl carrier protein reductase (2018), Antimicrob. Agents Chemother., 62, e00648 .
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
1.3.1.10 additional information deletion of the loop from Tyr96 to Val101 via site-directed mutagenesis results in the loss of triclosan resistance. The mutant FabL2 retains its enoyl-acyl carrier protein reductase activity uncultured bacterium pBF1

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.1.10 additional information triclosan is not inhibitory due to the presence of an extrapolated six-amino-acid loop specific to FabL2, which prevents the entry of TCL into the active site of FabL2. Elimination of this extrapolated loop via site-directed mutagenesis results in the complete loss of triclosan resistance but not enzyme activity uncultured bacterium pBF1

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.1.10 0.00963
-
 crotonyl-CoA pH 7.0, 25°C uncultured bacterium pBF1
1.3.1.10 0.0276
-
 NADPH pH 7.0, 25°C uncultured bacterium pBF1

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.10 uncultured bacterium pBF1 A0A1C9U547
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.10 crotonyl-CoA + NADPH + H+
-
 uncultured bacterium pBF1 butanoyl-CoA + NADP+
-
 ?
1.3.1.10 additional information the purified protein exhibits NADPH-dependent enoyl-acyl carrier protein reductases activity but no 7alpha-hydroxysteroid dehydrogenase activity, despite its high homology with 7-AHSDH uncultured bacterium pBF1 ?
-
-

Synonyms

EC Number Synonyms Comment Organism
1.3.1.10 Fabl2
-
 uncultured bacterium pBF1