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Literature summary extracted from

  • Matsuyama, T.; Tanaka, K.
    On the enzyme of Aspergillus fumigatus producing difructose anhydride I from inulobiose (1989), Agric. Biol. Chem., 53, 831-832 .
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.179 Co2+ 39% inhibition at 2.5 mM Aspergillus fumigatus
4.2.1.179 Cu2+ 67% inhibition at 2.5 mM Aspergillus fumigatus
4.2.1.179 dithiothreitol 14% inhibition at 2.5 mM Aspergillus fumigatus
4.2.1.179 L-cysteine 23% inhibition at 2.5 mM Aspergillus fumigatus
4.2.1.179 Zn2+ 36% inhibition at 2.5 mM Aspergillus fumigatus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.179 241
-
 bis-D-fructose 2',1:2,1'-dianhydride at pH 6.0 and 37°C Aspergillus fumigatus
4.2.1.179 377
-
 Inulobiose at pH 6.0 and 37°C Aspergillus fumigatus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.179 additional information Ca2+, Mg2+, and Mn2+ have no effect on the enzyme activity Aspergillus fumigatus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.179 bis-D-fructose 2',1:2,1'-dianhydride + H2O Aspergillus fumigatus i.e. difructose anhydride I inulobiose
-
 r
4.2.1.179 inulobiose Aspergillus fumigatus
-
 bis-D-fructose 2',1:2,1'-dianhydride + H2O
-
 r

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.179 Aspergillus fumigatus
-
-
-

Storage Stability

EC Number Storage Stability Organism
4.2.1.179 4°C, at pH 6.0 under toluene, several months, no loss of activity Aspergillus fumigatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.179 bis-D-fructose 2',1:2,1'-dianhydride + H2O i.e. difructose anhydride I Aspergillus fumigatus inulobiose
-
 r
4.2.1.179 bis-D-fructose 2',1:2,1'-dianhydride + H2O the equilibrium of the enzymic reaction is at about 85% in the cyclization and about 15% in the hydrolysis Aspergillus fumigatus inulobiose
-
 r
4.2.1.179 inulobiose
-
 Aspergillus fumigatus bis-D-fructose 2',1:2,1'-dianhydride + H2O
-
 r
4.2.1.179 inulobiose the equilibrium of the enzymic reaction is at about 85% in the cyclization and about 15% in the hydrolysis Aspergillus fumigatus bis-D-fructose 2',1:2,1'-dianhydride + H2O
-
 r

Synonyms

EC Number Synonyms Comment Organism
4.2.1.179 difructose anhydride I hydrolase
-
 Aspergillus fumigatus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.179 48
-
-
 Aspergillus fumigatus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.1.179 50
-
 the enzyme is stable up to 50°C at pH 6.0, with Tris-malate buffer Aspergillus fumigatus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.179 6
-
-
 Aspergillus fumigatus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.2.1.179 6 8 the enzyme is stable from pH 6.0 to 8.6 at 37°C Aspergillus fumigatus