Literature summary extracted from

Stines-Chaumeil, C.; Mavre, F.; Kauffmann, B.; Mano, N.; Limoges, B.
Mechanism of reconstitution/activation of the soluble PQQ-dependent glucose dehydrogenase from Acinetobacter calcoaceticus a comprehensive study (2020), ACS Omega, 5, 2015-2026 .

Metals/Ions

EC Number	Metals/Ions	Comment	Organism	Structure
1.1.99.35	Ca2+	reconstitution mechanism of the enzyme (sGDH) with its two cofactors, i.e., pyrroloquinoline quinone (PQQ) and Ca2+: pyrroloquinoline quinone first binds to apo-sGDH, it strongly impedes the access of Ca2+ to its enclosed position at the bottom of the enzyme binding site, thereby greatly slowing down the reconstitution rate of sGDH. The slow calcium insertion may purposely be accelerated by providing more flexibility to the Ca2+ binding loop through the specific mutation of the calcium-coordinating P248 proline residue, reducing thus the kinetic barrier to calcium ion insertion	Acinetobacter calcoaceticus

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.1.99.35	Acinetobacter calcoaceticus	P05465	-	-

Synonyms

EC Number	Synonyms	Comment	Organism
1.1.99.35	soluble PQQ-dependent glucose dehydrogenase	-	Acinetobacter calcoaceticus

Cofactor

EC Number	Cofactor	Comment	Organism	Structure
1.1.99.35	pyrroloquinoline quinone	reconstitution mechanism of the enzyme (sGDH) with its two cofactors, i.e., pyrroloquinoline quinone (PQQ) and Ca2+: pyrroloquinoline quinone first binds to apo-sGDH, it strongly impedes the access of Ca2+ to its enclosed position at the bottom of the enzyme binding site, thereby greatly slowing down the reconstitution rate of sGDH. The slow calcium insertion may purposely be accelerated by providing more flexibility to the Ca2+ binding loop through the specific mutation of the calcium-coordinating P248 proline residue, reducing thus the kinetic barrier to calcium ion insertion	Acinetobacter calcoaceticus

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)