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Literature summary extracted from

  • Mathieu, Y.; Offen, W.; Forget, S.; Ciano, L.; Viborg, A.; Blagova, E.; Henrissat, B.; Walton, P.; Davies, G.; Brumer, H.
    Discovery of a fungal copper radical oxidase with high catalytic efficiency toward 5-hydroxymethylfurfural and benzyl alcohols for bioprocessing (2020), ACS Catal., 10, 3042-3058 .
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.3.7 expression in Pichia pastoris Colletotrichum graminicola
1.1.3.47 expression in Pichia pastoris Colletotrichum graminicola

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.3.7 homology modeling of structure. The central, seven-bladed beta-propeller domain and the C-terminal, immunoglobulin-like beta-sandwich together constitute the AA5_2 catalytic module (residues Val199 to Leu689). The N-terminal domain contains conserved disulfide bridges and an overall fold typical of a PAN_1 domain Colletotrichum graminicola
1.1.3.47 homology modeling of structure. The central, seven-bladed beta-propeller domain and the C-terminal, immunoglobulin-like beta-sandwich together constitute the AA5_2 catalytic module (residues Val199 to Leu689). The N-terminal domain contains conserved disulfide bridges and an overall fold typical of a PAN_1 domain Colletotrichum graminicola

Protein Variants

EC Number Protein Variants Comment Organism
1.1.3.7 Y334F mutant exhibits specific activities comparable to the wild-type for carbohydrates, diols, aryl alcohols, 5-hydroxymethylfurfural and 5-hydroxymethyl-2-furancarboxylic acid Colletotrichum graminicola
1.1.3.7 Y334W mutant exhibits increased specific activity toward carbohydrates and decreased activity toward diols, aryl alcohols, and furans Colletotrichum graminicola
1.1.3.47 Y334F mutant exhibits specific activities comparable to the wild-type for carbohydrates, diols, aryl alcohols, 5-hydroxymethylfurfural and 5-hydroxymethyl-2-furancarboxylic acid Colletotrichum graminicola
1.1.3.47 Y334W mutant exhibits increased specific activity toward carbohydrates and decreased activity toward diols, aryl alcohols, and furans Colletotrichum graminicola

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.7 4.5
-
 4-Hydroxybenzyl alcohol wild-type, pH 7, 25°C Colletotrichum graminicola
1.1.3.7 6.5
-
 5-hydroxymethylfurfural wild-type, pH 7, 25°C Colletotrichum graminicola
1.1.3.7 14
-
 5-hydroxymethylfurfural mutant Y334F, pH 7, 25°C Colletotrichum graminicola
1.1.3.7 19
-
 4-Hydroxybenzyl alcohol mutant Y334F, pH 7, 25°C Colletotrichum graminicola
1.1.3.7 21
-
 3-anisyl alcohol wild-type, pH 7, 25°C Colletotrichum graminicola
1.1.3.7 24
-
 4-anisyl alcohol wild-type, pH 7, 25°C Colletotrichum graminicola
1.1.3.7 26.9
-
 5-(hydroxymethyl)furan-2-carboxylic acid wild-type, pH 7, 25°C Colletotrichum graminicola
1.1.3.7 27
-
 benzyl alcohol wild-type, pH 7, 25°C Colletotrichum graminicola
1.1.3.7 27
-
 5-(hydroxymethyl)furan-2-carboxylic acid mutant Y334F, pH 7, 25°C Colletotrichum graminicola
1.1.3.7 42
-
 5-(hydroxymethyl)furan-2-carboxylic acid mutant Y334W, pH 7, 25°C Colletotrichum graminicola
1.1.3.7 52.3
-
 3-anisyl alcohol mutant Y334F, pH 7, 25°C Colletotrichum graminicola
1.1.3.7 53
-
 5-hydroxymethylfurfural mutant Y334W, pH 7, 25°C Colletotrichum graminicola
1.1.3.7 61
-
 benzyl alcohol mutant Y334F, pH 7, 25°C Colletotrichum graminicola
1.1.3.7 88
-
 4-anisyl alcohol mutant Y334F, pH 7, 25°C Colletotrichum graminicola
1.1.3.47 6.5
-
 5-hydroxymethylfurfural wild-type, pH 7,25°C Colletotrichum graminicola
1.1.3.47 14
-
 5-hydroxymethylfurfural mutant Y334F, pH 7,25°C Colletotrichum graminicola
1.1.3.47 26.9
-
 5-(hydroxymethyl)furan-2-carboxylic acid wild-type, pH 7,25°C Colletotrichum graminicola
1.1.3.47 27
-
 5-(hydroxymethyl)furan-2-carboxylic acid mutant Y334F, pH 7,25°C Colletotrichum graminicola
1.1.3.47 42
-
 5-(hydroxymethyl)furan-2-carboxylic acid mutant Y334W, pH 7,25°C Colletotrichum graminicola
1.1.3.47 53
-
 5-hydroxymethylfurfural mutant Y334W, pH 7,25°C Colletotrichum graminicola

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.3.7 copper enzyme shows a copper-to-protein ratio of 1.08 Colletotrichum graminicola
1.1.3.47 copper enzyme shows a copper-to-protein ratio of 1.08 Colletotrichum graminicola

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.7 Colletotrichum graminicola E3Q9X3 cf. EC 1.1.3.47
-
1.1.3.7 Colletotrichum graminicola M1.001 E3Q9X3 cf. EC 1.1.3.47
-
1.1.3.47 Colletotrichum graminicola E3Q9X3 cf. EC 1.1.3.9
-
1.1.3.47 Colletotrichum graminicola M1.001 E3Q9X3 cf. EC 1.1.3.9
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.1.3.7 glycoprotein protein is predicted to be N-glycosylated on sites N309, N386, and N644 Colletotrichum graminicola
1.1.3.47 glycoprotein protein is predicted to be N-glycosylated on sites N309, N386, and N644 Colletotrichum graminicola

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.7 3-anisyl alcohol + O2
-
 Colletotrichum graminicola 3-anisaldehyde + H2O2
-
 ?
1.1.3.7 4-anisyl alcohol + O2
-
 Colletotrichum graminicola 4-anisaldehyde + H2O2
-
 ?
1.1.3.7 4-anisyl alcohol + O2
-
 Colletotrichum graminicola M1.001 4-anisaldehyde + H2O2
-
 ?
1.1.3.7 4-hydroxybenzyl alcohol + O2
-
 Colletotrichum graminicola 4-hydroxybenzaldehyde + H2O2
-
 ?
1.1.3.7 4-hydroxybenzyl alcohol + O2
-
 Colletotrichum graminicola M1.001 4-hydroxybenzaldehyde + H2O2
-
 ?
1.1.3.7 5-(hydroxymethyl)furan-2-carboxylic acid + O2
-
 Colletotrichum graminicola 2,5-formylfurancarboxylic acid + H2O2
-
 ?
1.1.3.7 5-hydroxymethylfurfural + O2
-
 Colletotrichum graminicola 5-(hydroxymethyl)furan-2-carboxylic acid + H2O2
-
 ?
1.1.3.7 benzyl alcohol + O2
-
 Colletotrichum graminicola benzaldehyde + H2O2
-
 ?
1.1.3.7 benzyl alcohol + O2
-
 Colletotrichum graminicola M1.001 benzaldehyde + H2O2
-
 ?
1.1.3.7 additional information enzyme is active on short-chain alkane diols and glycerol, and aryl alcohols, with similar specific activity values. Highest specific activity is observed on 5-hydroxymethylfurfural. Galactose and galactosylated oligosaccharides are poor substrates Colletotrichum graminicola ?
-
-
1.1.3.7 additional information enzyme is active on short-chain alkane diols and glycerol, and aryl alcohols, with similar specific activity values. Highest specific activity is observed on 5-hydroxymethylfurfural. Galactose and galactosylated oligosaccharides are poor substrates Colletotrichum graminicola M1.001 ?
-
-
1.1.3.7 veratryl alcohol + O2
-
 Colletotrichum graminicola veratryl aldehyde + H2O2
-
 ?
1.1.3.7 veratryl alcohol + O2
-
 Colletotrichum graminicola M1.001 veratryl aldehyde + H2O2
-
 ?
1.1.3.47 5-(hydroxymethyl)furan-2-carboxylic acid + O2
-
 Colletotrichum graminicola 2,5-formylfurancarboxylic acid + H2O2
-
 ?
1.1.3.47 5-hydroxymethylfurfural + O2
-
 Colletotrichum graminicola 5-(hydroxymethyl)furan-2-carboxylic acid + H2O2
-
 ?
1.1.3.47 additional information enzyme is active on short-chain alkane diols and glycerol, and aryl alcohols, with similar specific activity values. Highest specific activity is observed on 5-hydroxymethylfurfural. Galactose and galactosylated oligosaccharides are poor substrates Colletotrichum graminicola ?
-
-

Subunits

EC Number Subunits Comment Organism
1.1.3.7 ? x * 77500, SDS-PAGE, and x * 77507, calculated from sequence, deglycosylated protein Colletotrichum graminicola
1.1.3.47 ? x * 77500, SDS-PAGE, and x * 77507, calculated from sequence, deglycosylated protein Colletotrichum graminicola

Synonyms

EC Number Synonyms Comment Organism
1.1.3.7 GLRG_02805
-
 Colletotrichum graminicola
1.1.3.47 GLRG_02805
-
 Colletotrichum graminicola

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.3.7 25
-
 initial loss of 10% of activity, thereafter stable or at least 22 h Colletotrichum graminicola
1.1.3.47 25
-
 initial loss of 10% of activity, thereafter stable or at least 22 h Colletotrichum graminicola

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.3.7 3.7
-
 5-(hydroxymethyl)furan-2-carboxylic acid mutant Y334W, pH 7, 25°C Colletotrichum graminicola
1.1.3.7 15.4
-
 5-(hydroxymethyl)furan-2-carboxylic acid mutant Y334F, pH 7, 25°C Colletotrichum graminicola
1.1.3.7 20.1
-
 4-Hydroxybenzyl alcohol wild-type, pH 7, 25°C Colletotrichum graminicola
1.1.3.7 28.3
-
 5-(hydroxymethyl)furan-2-carboxylic acid wild-type, pH 7, 25°C Colletotrichum graminicola
1.1.3.7 33
-
 4-Hydroxybenzyl alcohol mutant Y334F, pH 7, 25°C Colletotrichum graminicola
1.1.3.7 48
-
 4-anisyl alcohol wild-type, pH 7, 25°C Colletotrichum graminicola
1.1.3.7 54.5
-
 benzyl alcohol wild-type, pH 7, 25°C Colletotrichum graminicola
1.1.3.7 71
-
 5-hydroxymethylfurfural mutant Y334W, pH 7, 25°C Colletotrichum graminicola
1.1.3.7 126
-
 5-hydroxymethylfurfural wild-type, pH 7, 25°C Colletotrichum graminicola
1.1.3.7 140
-
 3-anisyl alcohol wild-type, pH 7, 25°C Colletotrichum graminicola
1.1.3.7 201
-
 5-hydroxymethylfurfural mutant Y334F, pH 7, 25°C Colletotrichum graminicola
1.1.3.7 218
-
 4-anisyl alcohol mutant Y334F, pH 7, 25°C Colletotrichum graminicola
1.1.3.7 226
-
 benzyl alcohol mutant Y334F, pH 7, 25°C Colletotrichum graminicola
1.1.3.7 289
-
 3-anisyl alcohol mutant Y334F, pH 7, 25°C Colletotrichum graminicola
1.1.3.47 3.7
-
 5-(hydroxymethyl)furan-2-carboxylic acid mutant Y334W, pH 7,25°C Colletotrichum graminicola
1.1.3.47 15.4
-
 5-(hydroxymethyl)furan-2-carboxylic acid mutant Y334F, pH 7,25°C Colletotrichum graminicola
1.1.3.47 28.3
-
 5-(hydroxymethyl)furan-2-carboxylic acid wild-type, pH 7,25°C Colletotrichum graminicola
1.1.3.47 71
-
 5-hydroxymethylfurfural mutant Y334W, pH 7,25°C Colletotrichum graminicola
1.1.3.47 126
-
 5-hydroxymethylfurfural wild-type, pH 7,25°C Colletotrichum graminicola
1.1.3.47 201
-
 5-hydroxymethylfurfural mutant Y334F, pH 7,25°C Colletotrichum graminicola

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.3.7 7
-
 substrate5-hydroxymethylfurfural Colletotrichum graminicola
1.1.3.47 7
-
 substrate5-hydroxymethylfurfural Colletotrichum graminicola

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.3.7 5.5
-
 50% of the activity at pH 7.0, substrate 5-hydroxymethylfurfural Colletotrichum graminicola
1.1.3.47 5.5
-
 50% of the activity at pH 7.0, substrate 5-hydroxymethylfurfural Colletotrichum graminicola

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.3.7 0.088
-
 5-(hydroxymethyl)furan-2-carboxylic acid mutant Y334W, pH 7, 25°C Colletotrichum graminicola
1.1.3.7 0.45
-
 4-Hydroxybenzyl alcohol mutant Y334W, pH 7, 25°C Colletotrichum graminicola
1.1.3.7 0.57
-
 5-(hydroxymethyl)furan-2-carboxylic acid mutant Y334F, pH 7, 25°C Colletotrichum graminicola
1.1.3.7 0.66
-
 4-anisyl alcohol mutant Y334W, pH 7, 25°C Colletotrichum graminicola
1.1.3.7 0.75
-
 benzyl alcohol mutant Y334W, pH 7, 25°C Colletotrichum graminicola
1.1.3.7 0.85
-
 3-anisyl alcohol mutant Y334W, pH 7, 25°C Colletotrichum graminicola
1.1.3.7 0.97
-
 veratryl alcohol mutant Y334W, pH 7, 25°C Colletotrichum graminicola
1.1.3.7 1.1
-
 5-(hydroxymethyl)furan-2-carboxylic acid wild-type, pH 7, 25°C Colletotrichum graminicola
1.1.3.7 1.34
-
 5-hydroxymethylfurfural mutant Y334W, pH 7, 25°C Colletotrichum graminicola
1.1.3.7 1.74
-
 4-Hydroxybenzyl alcohol mutant Y334F, pH 7, 25°C Colletotrichum graminicola
1.1.3.7 2
-
 benzyl alcohol wild-type, pH 7, 25°C Colletotrichum graminicola
1.1.3.7 2
-
 4-anisyl alcohol wild-type, pH 7, 25°C Colletotrichum graminicola
1.1.3.7 2.47
-
 4-anisyl alcohol mutant Y334F, pH 7, 25°C Colletotrichum graminicola
1.1.3.7 3.7
-
 benzyl alcohol mutant Y334F, pH 7, 25°C Colletotrichum graminicola
1.1.3.7 4.4
-
 veratryl alcohol mutant Y334F, pH 7, 25°C Colletotrichum graminicola
1.1.3.7 4.5
-
 4-Hydroxybenzyl alcohol wild-type, pH 7, 25°C Colletotrichum graminicola
1.1.3.7 4.7
-
 veratryl alcohol wild-type, pH 7, 25°C Colletotrichum graminicola
1.1.3.7 5.5
-
 3-anisyl alcohol mutant Y334F, pH 7, 25°C Colletotrichum graminicola
1.1.3.7 6.6
-
 3-anisyl alcohol wild-type, pH 7, 25°C Colletotrichum graminicola
1.1.3.7 14.4
-
 5-hydroxymethylfurfural mutant Y334F, pH 7, 25°C Colletotrichum graminicola
1.1.3.7 19.4
-
 5-hydroxymethylfurfural wild-type, pH 7, 25°C Colletotrichum graminicola
1.1.3.47 0.088
-
 5-(hydroxymethyl)furan-2-carboxylic acid mutant Y334W, pH 7,25°C Colletotrichum graminicola
1.1.3.47 0.57
-
 5-(hydroxymethyl)furan-2-carboxylic acid mutant Y334F, pH 7,25°C Colletotrichum graminicola
1.1.3.47 1.1
-
 5-(hydroxymethyl)furan-2-carboxylic acid wild-type, pH 7,25°C Colletotrichum graminicola
1.1.3.47 1.34
-
 5-hydroxymethylfurfural mutant Y334W, pH 7,25°C Colletotrichum graminicola
1.1.3.47 14.4
-
 5-hydroxymethylfurfural mutant Y334F, pH 7,25°C Colletotrichum graminicola
1.1.3.47 19.4
-
 5-hydroxymethylfurfural wild-type, pH 7,25°C Colletotrichum graminicola