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Literature summary extracted from

  • Tang, X.; Liu, F.; Sheng, X.; Xing, J.; Zhan, W.
    Recombinant NADP-dependent isocitrate dehydrogenase of Edwardsiella tarda induces both Th1 and Th2 type immune responses and evokes protective efficacy against edwardsiellosis (2018), Vaccine, 36, 2337-2345 .
    View publication on PubMed

Application

EC Number Application Comment Organism
1.1.1.42 medicine the enzyme is a vaccine candidate against the fish pathogen Edwardsiella tarda infection. The serum of enzyme-vaccinated flounder exhibit the highest bactericidal activity compared with other groups. The recombinant enzyme can enhance the expressions of interferon-gamma, natural killer enhancing factor, interleukin-6, major histocompatibility complex Ialpha, CD4-1 and CD8alpha Edwardsiella tarda

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.42 expressed in Escherichia coli BL21(DE3) cells Edwardsiella tarda

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.42 membrane
-
 Edwardsiella tarda 16020
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.42 isocitrate + NADP+ Edwardsiella tarda
-
 2-oxoglutarate + CO2 + NADPH + H+
-
 r
1.1.1.42 isocitrate + NADP+ Edwardsiella tarda EIB202
-
 2-oxoglutarate + CO2 + NADPH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.42 Edwardsiella tarda D0Z8Y6
-
-
1.1.1.42 Edwardsiella tarda EIB202 D0Z8Y6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.42 His-Trap Ni-agarose column chromatography Edwardsiella tarda

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.42 isocitrate + NADP+
-
 Edwardsiella tarda 2-oxoglutarate + CO2 + NADPH + H+
-
 r
1.1.1.42 isocitrate + NADP+
-
 Edwardsiella tarda EIB202 2-oxoglutarate + CO2 + NADPH + H+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.42 ? x * 44000, SDS-PAGE Edwardsiella tarda
1.1.1.42 ? x * 45836, calculated from amino acid sequence Edwardsiella tarda
1.1.1.42 ? x * 65000, TRX-tagged enzyme, SDS-PAGE Edwardsiella tarda

Synonyms

EC Number Synonyms Comment Organism
1.1.1.42 IDH
-
 Edwardsiella tarda
1.1.1.42 NADP-dependent isocitrate dehydrogenase
-
 Edwardsiella tarda

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.42 NADP+
-
 Edwardsiella tarda

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.1.1.42 Edwardsiella tarda calculated from amino acid sequence
-
 5.2